FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C10a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C10a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140185
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGACTGTTGAATACATTGCAGCAAACTTGTTAGTTTCGATTTTCG163541.434328639650583No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC160171.4047720326087432No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG146491.2847915031332635No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA132371.1609519507799173No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG131561.1538478404820272No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA123821.0859641198577423No Hit
GTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA109220.9579147243649058No Hit
GTTGAATACATTGCAGCAAACTTGTTAGTTTCGATTTTCGGATCTTCCCC106390.9330941908549929No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA67320.5904305003135456No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT62790.5507001056846038No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGA58110.5096541350745712No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATGTATT56610.49649837526366336No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT56300.49377951823607574No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG54450.47755408113595604No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG52420.4597499528585273No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC48050.42142283927608243No Hit
CTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATT46800.41045970610032584No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT46770.41019659090410765No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCC42660.37414980902222006No Hit
ACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGAAGCAATCGAG42170.3698522608173235No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC41790.36651946833189347No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA41540.3643268416967422No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATGTA40290.35336370852098564No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA39960.3504694413625859No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC39500.34643500835390745No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT38390.33669974609383563No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG35990.315650530396383No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT35920.3150365949385407No Hit
CGCATATAGACTGTTGAATACATTGCAGCAAACTTGTTAGTTTCGATTTT35720.3132824936304196No Hit
GTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGG35180.3085464200984928No Hit
GAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAA32560.28556769296210704No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT30000.2631151962181576No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA29870.2619750303678789No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG29800.2613610949100365No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG29170.2558356757894552No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG29160.25574797072404915No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA27790.24373237676341997No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCA27740.24329385143638968No Hit
TCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATG27710.24303073624017155No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT26550.23285694865306947No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC26470.23215530812982105No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA26230.2300503865600758No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA25870.22689300420545788No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA25760.2259282484859913No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT25490.22356021172002788No Hit
ATCTTGGGGGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCC25170.2207536496270342No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA24680.21645610142213764No Hit
AAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAAT24650.2161929862259195No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG23490.20601919863881737No Hit
GGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTT23130.2028618162841995No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA22690.1990027934063332No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATAT22690.1990027934063332No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA22530.19759951235983633No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA21960.19260032363169136No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA21660.18996917166950977No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT21280.18663637918407977No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA21060.1847068677451466No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC20900.18330358669864977No Hit
ATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGC20830.1826896512408074No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA20810.1825142411099953No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC20720.18172489552134083No Hit
ACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAG20540.1801462043440319No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA19360.16979700662611769No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA19300.1692707762336814No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACA19270.1690076610374632No Hit
CTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA19220.16856913571043297No Hit
GTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACT19190.16830602051421478No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA18990.16655191920609375No Hit
TCATTGTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTT18160.1592723987773914No Hit
ATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATTGC18140.15909698864657928No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC17850.15655354174980374No Hit
CTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATG17820.1562904265535856No Hit
GTCATAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACT17610.1544486201800585No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC17560.15401009485302822No Hit
GAAGTAGTTGTGGCAATGCTACTATTTGCTATCCATACTGTCCAAAAAAG17470.15322074926437376No Hit
GTTTTCGGCTGAATCTAGAAAATTGCTTCTCATTGTTCAGGCACTTAGGG17390.15251910874112534No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAC17260.15137894289084666No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT17060.1496248415827256No Hit
ATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAAT16870.1479584453400106No Hit
TCCCCATATTCTTTCATTGCCTTTTCCGCAAGCTCGACGATCATTGGATT16620.1457658187048593No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16480.14453794778917456No Hit
TTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCA16470.14445024272376852No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA16260.14260843635024142No Hit
TGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAA15900.13945105399562352No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTT15420.135241210856133No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT15180.13313628928638774No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA15150.13287317409016958No Hit
TTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGACT15000.1315575981090788No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT14720.12910185627770931No Hit
CACATGCACTGAAGTAGTTGTGGCAATGCTACTATTTGCTATCCATACTG14700.12892644614689722No Hit
CTTGAGGGGTTTTCGGCTGAATCTAGAAAATTGCTTCTCATTGTTCAGGC14610.12813710055824273No Hit
ATCTAGAAAATTGCTTCTCATTGTTCAGGCACTTAGGGACAACCTGGAAC14460.12682152457715196No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT14460.12682152457715196No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA14390.12620758911930957No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT14280.12524283339984302No Hit
GTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAA13950.12234856624144329No Hit
GGATAAAGAACAGGCGTGACAGCTGCTTCCTAAATAATCAACGGTGGGTG13830.12129610545657063No Hit
CTGTTGAATACATTGCAGCAAACTTGTTAGTTTCGATTTTCGGATCTTCC13770.12076987506413432No Hit
GTTCAACTCCTTCCTCACACATGCACTGAAGTAGTTGTGGCAATGCTACT13740.12050675986791617No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT13240.11612150659761356No Hit
TTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTC13000.11401658502786828No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT12960.11366576476624407No Hit
CCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAAC12920.11331494450461986No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCAT12900.11313953437380776No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA12470.10936821656134751No Hit
ACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAG12450.1091928064305354No Hit
CCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGG12360.10840346084188092No Hit
AAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTT12240.1073510000570083No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA12150.10656165446835382No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC12040.10559689874888724No Hit
GACGCATATAGACTGTTGAATACATTGCAGCAAACTTGTTAGTTTCGATT11880.1041936177023904No Hit
GTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTT11870.10410591263698434No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11810.10357968224454803No Hit
AATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATGTAT11680.10243951639426936No Hit
CAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCT11660.10226410626345724No Hit
GGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGA11490.10077312015155436No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)