FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5450829
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAG661241.2130998789358463No Hit
ACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCA440590.8082990679032491No Hit
CCCTTATTTCTCTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAA320810.5885526770331632No Hit
TCTCAGAAGAGTATCAAGAACAATTCAGAACATACAATATGTGTGTGATA313800.5756922479131156No Hit
GGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAA286320.5252778980958676No Hit
GTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTA253630.46530536914660137No Hit
CTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTA247380.4538392233548328No Hit
ACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTT240480.4411805984007203No Hit
GTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTA224100.4111301235096533No Hit
ATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTG223510.41004771934691037No Hit
TAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTAGGAA218570.40098487771309643No Hit
GGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCT208640.38276746527913463No Hit
ATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTT199590.36616448617265374No Hit
CAAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTC179830.3299131196373983No Hit
ATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGG176110.3230884696621376No Hit
ATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAG174430.32000636967331025No Hit
CAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAG168820.3097143572106188No Hit
ATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCATAATCA139160.25530061574120194No Hit
CTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGA134110.24603596994145294No Hit
CCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAA131450.24115597829247626No Hit
GTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAAATCATAG127680.23423959915088144No Hit
ACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTG125530.2302952449985131No Hit
ATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCTGTGGGAACTGAGTAA116400.21354549922589758No Hit
TACTAATACAGACCAGGGAAAGAAGGAGGAGGGAGAGGGGATGAGAAGAG114890.2107752784026063No Hit
GATTATAAAAGGTCACCAAAAAGGACAAAAAACATTAAAAAGCAATGTCC103470.1898243368118868No Hit
AAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCC102370.1878062951525355No Hit
CTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAG100380.1841554743324364No Hit
CATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCAC95160.17457894936715132No Hit
ATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCAGT91780.16837805772296285No Hit
AAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGG89520.16423189940465935No Hit
GTTTTTAAGTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCT88170.16175521191363734No Hit
TCCTAACACGTAGTACCTCAAATCATAGGTGATTCAGGTGGTTTAGTTAT85070.15606800360092016No Hit
ATATAAAACAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATACACAC84990.15592123693478552No Hit
TTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACC81110.1488030536272556No Hit
TATATAACCAGAACTAAAAATCAGGTATCATTCTTGTCCCATTTACTAAA79500.145849374471296No Hit
GTATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACT79170.14524396197349065No Hit
TTATTAACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACT78960.14485869947488722No Hit
ACTTTATGCTTTAAGAAAGTTTTGGGAAGTTTCTGATAAAATATTGGTTC76050.13952006199423977No Hit
GTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCTATCATCAC75050.13768547866755682No Hit
GTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGC75000.13759374950122266No Hit
TTCTAGGACATTGCTTTTTAATGTTTTTTGTCCTTTTTGGTGACCTTTTA73940.1356490911749387No Hit
GAAATAAGGGAGGACCTCTCTTTGCCTCCAAATATTCATCCAGTAGGTGG73420.13469510784506356No Hit
CCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAG72760.13348428284945282No Hit
GTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGCCCCTTGA72180.13242022451997668No Hit
GAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGG71610.1313745120237674No Hit
CATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAA69770.12799887870267074No Hit
GTATTATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCAT68650.1259441453767858No Hit
GTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCA68420.12552219121164873No Hit
ACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAG67930.12462324538157407No Hit
CTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTT66650.12227497872341986No Hit
GAATGAAGGGTGACTGCAAATGAGCACAAAGATCATTCTGGGATGATGAA64780.1188443079025227No Hit
TCTCTAATGTATCAGCCACTGAGTCATCCTTCACATTGGAATTACTGATT64360.11807378290531587No Hit
GATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGAT64130.11765182874017878No Hit
GGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGT59960.11000161626791082No Hit
ATTTTACTCAGTTCCCACAGAAGTACTCTTAAAAAAACCCAAACTTCCTT59880.10985484960177617No Hit
GGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAA59860.10981815793524251No Hit
AACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAA59670.10946958710317276No Hit
AATATTAACAGCCTGAAAAGGATTCTTGATTACTACCATTCATCAACTAT59310.10880913710556689No Hit
ACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCA58640.1075799662766893No Hit
TATTTATACCTGTCTTTCCACATACTTTTTTCAAATGGTTTCATGAAAAG58040.10647921628067951No Hit
ATTCTGGGATGATGAAAATGTTCTAAAAATAAACTGTGATGATAGCTGCA57590.10565365378367217No Hit
ATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACC57390.10528673711833558No Hit
AATGTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGAC57360.1052316996185351No Hit
CTTGTAAACCTGGAATAATGTTCCATTAACTGGATATGATTGCATAATTA56770.10414929545579214No Hit
CTTGAGAACTGATACCTTAGCCCCTTGATGATGATTACAGAGAAATAAGG56450.1035622287912536No Hit
GAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAA55890.10253486212831113No Hit
ATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTACT55840.10244313296197698No Hit
ATATTACGTAGTGTGTATACACACACACACACACACACACACACACACAC55720.10222298296277502No Hit
GTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTAT55470.10176433713110428No Hit
AGGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCA54980.10086539130102963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)