FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006438
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTA95900.9528654522186165No Hit
CCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAG70120.696714551716052No Hit
ATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGA65180.6476305544901921No Hit
CCCCCACCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAG65160.6474318338536502No Hit
GCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAA64570.6415695750756628No Hit
AAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCT59350.5897034889382158No Hit
GGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATG53050.5271064884275037No Hit
GTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGG51770.5143883676888195No Hit
GGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGAT49650.4933239802153734No Hit
TCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAG49510.49193293575957986No Hit
GTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTAC48390.480804580113231No Hit
GTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTT46360.4606344355042238No Hit
CTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCTTCATCTCA46100.4580510672291785No Hit
GAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCT45780.4548715370445075No Hit
GGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTC45160.4487111973117072No Hit
CTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCA44150.4386758051663391No Hit
AGCGAAAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGC41550.4128421224158865No Hit
GATGAAGGTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGT40100.3984348762665957No Hit
GGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAAAAATGCTGTT36110.35879010927647803No Hit
AATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACA34180.33961356785018054No Hit
CTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAGGGCTTCAGAG31470.3126869215987473No Hit
CAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTG30990.30791762632174063No Hit
AGCGAAAGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGC30900.3070233834573019No Hit
GGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGC28180.2799973768875977No Hit
GTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTG27080.2690677418777908No Hit
GCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTTTG26690.2651926894652229No Hit
CTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACAC25900.2573432243218161No Hit
CTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTAT25600.254362414773687No Hit
GTAGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAAC24690.2453206258110286No Hit
AGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCA24470.2431346988090672No Hit
GGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCA24230.24075005117056392No Hit
AAAGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAG23970.23816668289551862No Hit
GTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCC23750.2359807558935573No Hit
CCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACA23670.2351858733473895No Hit
AGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCT23170.23021785743384096No Hit
TGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAA23140.22991977647902803No Hit
GCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAA22050.21908950178749212No Hit
GTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAA21600.2146182874652984No Hit
GGTAAATATATTTTTAAAAACTTTACTCTGGGGCGCCTGGGTGGCTCAGT21390.212531720781608No Hit
CTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTA21160.21024643346137564No Hit
GGTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTA20880.2074643445497885No Hit
GGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGC20690.20557649850264No Hit
ACCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAA20530.20398673341030446No Hit
CAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTT20140.20011168099773657No Hit
GCCTAACAAGGGGTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGA19780.19653470953998162No Hit
TCCCTGAACCTTCATCTCACTGAAAACCAGGTCCTGATTAAACCTAACAT19730.19603790794862674No Hit
CAAGTAGGCAGAGAGGCAGGCAGAGAGAGAGAGGAGGAAGCAGGCTCCCT19530.1940507015832073No Hit
CTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAG19350.19226221585432982No Hit
TTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAG19170.19047373012545235No Hit
GCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCAT19130.19007628885236846No Hit
GGGTGGGGGAATGCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTT18940.18818844280521999No Hit
CCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCT18820.18699611898596835No Hit
GGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTTGAG18680.18560507453017475No Hit
GTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAG18670.18550571421190376No Hit
TCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTT18660.18540635389363277No Hit
ACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGG18220.18103449988971004No Hit
CTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTC17420.17308567442803233No Hit
GGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAAAAAT17370.17258887283667748No Hit
GTTTCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGA16850.16742213628658695No Hit
ATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAA16740.16632917278560627No Hit
CAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGC16030.1592745901883673No Hit
TTGTTAGGCTCCCTGCTCAGCTGGGAGTCTGCTGCTTCCTGTCCCTCTGC15840.15738674414121884No Hit
CTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGGA15600.15500209650271551No Hit
GTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAGGG15140.15043152186225084No Hit
GGGAATGCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTC15130.15033216154397985No Hit
GGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTT15080.149835359952625No Hit
TCCCAGGACCCGGAGATCAAGACCTGAACTGAAGGCAGACACTTAACCTA14690.1459603075400571No Hit
GGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATC14570.14476798372080546No Hit
CCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGA14530.14437054244772157No Hit
GACAGAGAGAGACCACAAGTAGGCAGAGAGGCAGGCAGAGAGAGAGAGGA14400.14307885831019893No Hit
GGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGA14020.13930316621590202No Hit
GTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCTCAGCTGGGAGTCTGC13870.13781276144183746No Hit
TAACAAGGGGTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGAAGG13610.1352293931667922No Hit
GTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCTC13570.1348319518937083No Hit
GCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATC13150.1306588185263275No Hit
CTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGC13090.13006265661670166No Hit
GGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCT13080.1299632962984307No Hit
ACTTTACTCTGGGGCGCCTGGGTGGCTCAGTGGGTTATGCCGCTGCCTTC12920.12837353120609515No Hit
ATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAA12820.12737992802338546No Hit
GGCAGAGGGAGAGGGGGAAGCAGGCTTCGGGCTGAGCAAGGAGCCGGATA12710.12628696452240476No Hit
GTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGG12560.1247965597483402No Hit
GCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAG12530.12449847879352728No Hit
GGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCTTCATCTCACTGA12420.12340551529254659No Hit
GTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCC12190.12112022797231424No Hit
ACTCTGGGGCGCCTGGGTGGCTCAGTGGGTTATGCCGCTGCCTTCGGCTC12150.12072278669923037No Hit
GCGAAAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCC11950.11873558033381093No Hit
TCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAAC11730.11654965333184955No Hit
GCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTT11670.11595349142222372No Hit
AGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGAT11200.11128355646348807No Hit
AAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCC11110.11038931359904933No Hit
CTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCTTCATCTCACT11070.10999187232596543No Hit
GTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCC10960.10889890882498474No Hit
GGTCATGATCTCAGGGTCCTGGGATCGAGTCCCGCATCGGGCTCTTTGCT10910.10840210723362989No Hit
GAGCCTAACAAGGGGTTCCATCCCAGGACCCGGAGATCAAGACCTGAACT10900.1083027469153589No Hit
GTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGAAGGCAGACACTT10810.10740850405092017No Hit
GCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGGAGATCAA10700.1063155405499395No Hit
AAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAAT10460.10393089291143617No Hit
GGGTTATGCCGCTGCCTTCGGCTCGGGTCATGATCTCAGGGTCCTGGGAT10080.10015520081713926No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)