FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5403039
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA198640.3676449494441924No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT156910.29041063742090334No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT148750.27530802572404156No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC142070.26294461320749307No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT118640.21958012888672468No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC109820.20325598242026385No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG104780.19392789872514338No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC102280.18930087308272253No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT91930.17014498692310012No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC90840.16812760374300462No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA89510.16566602610123674No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG85440.15813322835537555No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT84420.15624540189326783No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA84300.15602330466243164No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA81140.15017474425041166No Hit
CTTCTAGGTATTTATTTCTTCTCTCATCAAAAGCAGAAAGCACCATCCTC79880.14784272332663156No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC75380.139514077170274No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG74120.13718205624649388No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA73950.13686741850280926No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC73830.13664532127197307No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA72450.13409120311735673No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT71600.13251801439893363No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA70460.13040809070598972No Hit
CCTTACAACTACTGCTTGAAGTAGAACAAGAGATAAGAGCTTTCTCGTTT69200.12807606978220962No Hit
CATTTATGCAAGCCTTACAACTACTGCTTGAAGTAGAACAAGAGATAAGA68320.12644735675607746No Hit
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC68010.12587360557641727No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT67420.12478162752480595No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA67200.1243744492682729No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA66880.12378218998604304No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC65150.12058028824148781No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG64710.11976593172842173No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA64630.11961786690786426No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT63840.11815572680485927No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA63590.11769302424061719No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA62230.11517592229114022No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA60940.11278837705965106No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC60310.11162236659776101No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG59130.10943841049453834No Hit
GTAATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA58870.10895719982772659No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT57820.10701384905790981No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC57120.10571828187803198No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT55320.10238682341548895No Hit
GGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTT55320.10238682341548895No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT55020.10183158033839844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)