FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6921426
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT429760.6209125113813252No Hit
GGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGA424110.6127494536530478No Hit
AACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCC282730.408485187878914No Hit
CCCTATGCCATTGGGCAAGGGGCATTAGCCTGCAGAAAGGATCTTGCTGT268550.38799808016440546No Hit
AGTCAGGAAACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGA263380.380528521145787No Hit
AGCAGGGACAGAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCC252660.3650403832967368No Hit
TAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAG246260.3557937338346173No Hit
AAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTATGGGC245180.3542333617378846No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC237920.3437441937542928No Hit
GTCCTGGGTGGCAGTCCTATCGGGGCACCATAGGGGCAATTGCAGCCTGT231780.33487318942657196No Hit
TCTATACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGGCA226350.32702798527355487No Hit
GTATAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGC221140.31950063469579826No Hit
CAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCCTT211570.30567400417197266No Hit
ATGTAAAGAAGCTTAAAACCACTTACCTAAAGTCTTGCTTTATAATCAGT203930.2946358163765675No Hit
ACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAGCA190930.2758535596566372No Hit
CTCCCTGCCCAGTCCTCTTCCCATCCTCATCCACAGGCTGCAATTGCCCC186620.2696265191594911No Hit
AATCTATACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGG182020.26298048985859274No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA181400.2620847206919499No Hit
GAACTAGAACAAATAATCCTAAAATTTGTATGGAACCACAAAAGAGCCTG177040.255785440745881No Hit
GCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAACT163900.23680091356896688No Hit
GCCATAGGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCA161680.23359348203679414No Hit
GCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTC161440.2332467326819647No Hit
AAATAAAACTTCTTTGAAGGAGACAGAATCCTTTTTAGCAATAAGGACAC160790.23230761984596818No Hit
TAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGA153060.22113940104250196No Hit
TTTCTGATGAGAGTGAGCACTGGTTTCACTGGTTTTTCATGATGTAAAGA145480.21018790058580417No Hit
GTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTG145410.2100867653573122No Hit
GAATAAAACTGTCAAAAAATAAAGAAAGTGACTTAGAGCAGGATCCATAA145390.21005786957774308No Hit
CTGTATAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTA137340.1984273183011709No Hit
AGTAGAAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTA131410.18985971965892579No Hit
CCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAG128080.1850485723606667No Hit
GCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGAA127430.1841094595246702No Hit
CCATAGGGGCAATTGCAGCCTGTGGATGAGGATGGGAAGAGGACTGGGCA126960.1834304087047958No Hit
ACTTAGAGCAGGATCCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAAC126900.18334372136608845No Hit
ATATTGAGATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGGTTT126850.18327148191716564No Hit
CCCCTATGGTGCCCCGATAGGACTGCCACCCAGGACTGTGGCCCATAATC125840.1818122450489249No Hit
AGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAAC125650.18153773514301821No Hit
TCTTTACATCATGAAAAACCAGTGAAACCAGTGCTCACTCTCATCAGAAA124220.1794716869038259No Hit
GGAAACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAA124030.17919717699791923No Hit
ACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTT123490.17841699094955288No Hit
CTCTGAAACTGTACGCAAATTTGCATAATGTGCATTTTTCTGATGAGAGT121840.17603308913510018No Hit
GAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCCTCTTCCCATC119040.17198767999542292No Hit
GTCCTCTTCCCATCCTCATCCACAGGCTGCAATTGCCCCTATGGTGCCCC111390.16093504431023317No Hit
GTATTTCTTTCTCAGTGTCATGAATTGTGGTTAATGCTTGCTAGAAAAGC110010.15894123551996364No Hit
ACCCAGGACTGTGGCCCATAATCCCAGCTTTTACTATGCCTATGGCTCCA107330.1550692010577011No Hit
ACCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTAT107120.15476579537222532No Hit
GTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATT105410.15229520621906525No Hit
GTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGG104760.15135609338306874No Hit
GACTTAATATGTTGCAGAGAAGGTAGTGTTAATGAACCGAAGTTAGAGGT104570.15108158347716208No Hit
GTATGTTTGTTTTGACTCCTTTTAAAAAGAGTAGAGCATAAACTATCTGG103880.1500846790820273No Hit
GTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTCGGTTTGCAGTTC103830.1500124396331045No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT103750.149896856514828No Hit
CTTCCCATCCTCATCCACAGGCTGCAATTGCCCCTATGGTGCCCCGATAG103290.14923225358473818No Hit
ACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCCTTTTTAG102980.14878436900141676No Hit
GTTCCAGTCACCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTT102530.14813421396111148No Hit
GAGCTAGACTCGGTTTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCT100250.1448400950902314No Hit
GAGTAGAGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATG98520.1423406101575022No Hit
GGTCTGAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAA97420.14075134228120043No Hit
TTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTTTCC96380.139248761743606No Hit
TAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTATCGG95320.13771728542644246No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC94700.13682151625979963No Hit
CTAGAAAACACTGATGAAAGAAATTGAAGATGGCACAAACAAACGGGAAG94460.13647476690497015No Hit
AGCTGGACGAGTCAGGAAACAAGTACTCTGGACCAAAAATAAAACTTCTT93870.135622341407681No Hit
GGTTAGGCCTTTAAGCTAAGCTTGGTGAAAAGGAGTTTTAGGATAGAACT93320.13482770746953013No Hit
GGACAGAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCCTCTTC93090.1344954060044852No Hit
GGGCTAGCTGGACGAGTCAGGAAACAAGTACTCTGGACCAAAAATAAAAC92110.13307951280559815No Hit
GGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTA89430.1292074783433356No Hit
ATCAAAATACCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAA89280.12899075999656717No Hit
GAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAAAGCCA89070.12868735431109138No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA88070.12724256533263523No Hit
GTAGAAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTAT86760.12534989177085762No Hit
ATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGGTTTTAAGCTTC86510.12498869452624357No Hit
GTCATGAATTGTGGTTAATGCTTGCTAGAAAAGCGTGGAATACTTGAACT86340.12474308039990603No Hit
CCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAACTGTACGCAAATTTG85220.12312491674403511No Hit
CTCTTAATCTCTGAAACTGTACGCAAATTTGCATAATGTGCATTTTTCTG84830.1225614490424372No Hit
TTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGCCT84790.12250365748329897No Hit
TTATAAAGCAAGACTTTAGGTAAGTGGTTTTAAGCTTCTTTACATCATGA84710.12238807436502246No Hit
ATGGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAA84390.12192574189191649No Hit
CCACAAAAGAGCCTGAATAGCCAAACAACCTTAAAAAAGGAAAGCAAATC84300.12179571088385545No Hit
GAGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAA84150.12157899253708701No Hit
CTTAATGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAA81960.11841490467426799No Hit
CAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTATGG80520.1163344085452911No Hit
TAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGT79930.11548198304800197No Hit
AGCGAAAGCAGGGCTAGCTGGACGAGTCAGGAAACAAGTACTCTGGACCA79720.11517857736252615No Hit
GCTAAAAAGGATTCTGTCTCCTTCAAAGAAGTTTTATTTTTGGTCCAGAG78760.11379157994320824No Hit
CTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAGCATTAACC78760.11379157994320824No Hit
AATTTGCATAATGTGCATTTTTCTGATGAGAGTGAGCACTGGTTTCACTG77950.11262130087065873No Hit
CCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTATG76780.11093089776586501No Hit
TCCTAGTGATTAGGAAGTTCAGACTGTATTTCTTTCTCAGTGTCATGAAT76670.11077197097823484No Hit
AAGCTAAGCTTGGTGAAAAGGAGTTTTAGGATAGAACTGCAAACCGAGTC76510.11054080474168185No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA75490.10906711998365656No Hit
GTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTTTCCTTTTT74360.10743450843800108No Hit
GCCCAATGGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCT74270.10730447742994002No Hit
ATCCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAACTGTACGCAAATT73260.10584524056169928No Hit
AGATATCCCTTATAGGAAAAACCTCTAACTTCGGTTCATTAACACTACCT73170.10571520955363822No Hit
AGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTATCGGG72740.10509395029290208No Hit
GTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTATCGGGG72280.10442934736281223No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT72010.10403925433862907No Hit
GCAATATTGAGATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGG71760.10367805709401501No Hit
CTCCCTATGCCATTGGGCAAGGGGCATTAGCCTGCAGAAAGGATCTTGCT71370.1031145893924171No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT70400.10171314408331461No Hit
CCTTTAAGCTAAGCTTGGTGAAAAGGAGTTTTAGGATAGAACTGCAAACC70180.10139529050805426No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA69600.10055731290054969No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)