FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316061
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAATACAGTTCCGATCTTATTAGTTAAGACAGAATCTGTCTGTTCCA38320.2911719137638757No Hit
GTTCAATGAAATGTTCAAATAAGGAAAGATGAAGTTCATATGGTGCATGG38210.29033608624524243No Hit
TCATTGAACTGCTCACTGAGTCCAGTGAAGCTTCAAAGAATGCCAAATTA37020.2812939521800281No Hit
ATCTAGAAGTCTATTCCTCAAAAGTATAAGATTAGCAGATACAAGACCTC36770.27939434418313436No Hit
GTTTATTTCTTCTACTTTACCAACGTTTGCAGTTTGTGAGAAATTTGTAG34770.2641974802079843No Hit
GTATAAGATTAGCAGATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTA34640.26320968404959955No Hit
CCATATGAACTTCATCTTTCCTTATTTGAACATTTCATTGAACTGCTCAC30810.23410768953718708No Hit
TCACTGGACTCAGTGAGCAGTTCAATGAAATGTTCAAATAAGGAAAGATG29150.22149429243781255No Hit
GTCCAGTGAAGCTTCAAAGAATGCCAAATTAATGAGGGAATTCCAGCTAA26800.2036379772670112No Hit
CTTTAGATGTGTAAACTAGTTTTAGCAAGATATCTAGAAGTCTATTCCTC26080.19816710623595715No Hit
GCATACATACGGCCTGAAAGCTTCCAACATAATTTGGTCCTAGTTCATAA25990.1974832473570754No Hit
CTGTTGATAGTGGGCATGAGACCTCCATTATTCCAAATTCAACTGCTTTC25650.19489978048129988No Hit
TCCATAAACATCATAATCCAGTCAAAACCCAGTGTCCTGACCAGTTCTTC25530.19398796864279086No Hit
GTCTAAGGGAAAGCCGTGACCCCGGGGTCCAGCAGGTGCCTGCCCTGTGC25160.19117654880738813No Hit
GAAATAAACAATGAAGAGAAGCTTGATACTGGAACTGAAGAAGAGTTTGG24890.18912497217074284No Hit
ATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTATCAAGCTTCTCTTCA24250.18426197569869482No Hit
GTTTATTTCCATAACTACAAATTTCTCACAAACTGCAAACGTTGGTAAAG23520.17871512034776504No Hit
ACTCATAGCAGCAATGGTAGGTTGGGATAAAGACATATCTCGAAGAGTCA23190.17620763779186527No Hit
CTTCTAGATATCTTGCTAAAACTAGTTTACACATCTAAAGAAAAGACAAG23150.17590370051236226No Hit
CTATCAACAGTTCCCACCAAGGAAAATGTTAGATGGAGAATGTGGCTGTT22410.17028086084155675No Hit
GTTCTTCACAAGCTTGCAAATTAATGCTTGTCTTTTCTTTAGATGTGTAA21990.16708951940677522No Hit
CCATTATTCCAAATTCAACTGCTTTCCAGGATCTACTTTGTGATTTTGAA21720.16503794277012995No Hit
TATGTATGCCGTGTAAAGATACAAAGTCTGAAGGCGTAGTCCCATCTCCT21580.16397416229186945No Hit
GAATAAGCCTGGCCTCAGTTTTTCTGACGCGACAGAGGTTTTGGGCACTC20010.15204462407137664No Hit
GGATAAAGACATATCTCGAAGAGTCAGTAGCAATTTGGGGATTAGCTGGA19490.1480934394378376No Hit
GTCTATGCGTACATTGGCACTCCACCTGCCCAGCGTCAAATTGCTTCCTT19010.14444619208380158No Hit
ATCTTATACTTTTGAGGAATAGACTTCTAGATATCTTGCTAAAACTAGTT18890.14353438024529258No Hit
GTGTAAACTAGTTTTAGCAAGATATCTAGAAGTCTATTCCTCAAAAGTAT18850.14323044296578957No Hit
GTCTTGTATCTGCTAATCTTATACTTTTGAGGAATAGACTTCTAGATATC18630.14155878792852306No Hit
TTACCAACGTTTGCAGTTTGTGAGAAATTTGTAGTTATGGAAATAAACAA18440.1401150858508838No Hit
GCACGGGAGAATCTCATAAAAAGAAGATTCTTCACTAAAATCATCAACAT18300.1390513053726233No Hit
GTATCAAGCTTCTCTTCATTGTTTATTTCCATAACTACAAATTTCTCACA18190.13821547785399005No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT18030.13699972873597804No Hit
GTCCTGACCAGTTCTTCACAAGCTTGCAAATTAATGCTTGTCTTTTCTTT18010.13684776009622654No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT18000.13677177577635077No Hit
ACTATAAGTTCTCCACATCGAAAGCGGGCACAGCACGGGAGAATCTCATA17380.13206074794405429No Hit
AATTAATGCTTGTCTTTTCTTTAGATGTGTAAACTAGTTTTAGCAAGATA17040.12947728106827874No Hit
TCATAATCCAGTCAAAACCCAGTGTCCTGACCAGTTCTTCACAAGCTTGC17030.129401296748403No Hit
ATATGAACTTCATCTTTCCTTATTTGAACATTTCATTGAACTGCTCACTG16570.12590601803411847No Hit
CATCTAAAGAAAAGACAAGCATTAATTTGCAAGCTTGTGAAGAACTGGTC16480.1252221591552367No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA16340.12415837867697622No Hit
CGCATAGACTGTGCTGACCACAGGTGGGTTAGCGGAACCTGAGCCACCAG16130.12256270795958546No Hit
CTAGATATCTTGCTAAAACTAGTTTACACATCTAAAGAAAAGACAAGCAT16060.12203081772045521No Hit
CTCCTTAACAGCCACATTCTCCATCTAACATTTTCCTTGGTGGGAACTGT15880.1206630999626917No Hit
AACCTGAGCAGATCATTGCTGTTGGGAAAACCTTGTAGTAAGAAGCTCAG15710.11937136652480394No Hit
GGATAGACTGATTACTCAGTAGCACAACAAGAATCCTCATGGCTGCCGTA15700.1192953822049282No Hit
GCTCAGGTTTGGCCAGTTTATTTCTTCTACTTTACCAACGTTTGCAGTTT15670.11906742924530093No Hit
GTTCATATGGTGCATGGAGCCAAACTTCAAAATCACAAAGTAGATCCTGG15640.11883947628567369No Hit
GATATCTAGAAGTCTATTCCTCAAAAGTATAAGATTAGCAGATACAAGAC15320.11640797804964968No Hit
AGTTTACACATCTAAAGAAAAGACAAGCATTAATTTGCAAGCTTGTGAAG15200.11549616621114066No Hit
GATTAGCAGATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTATCAAGC14920.11336860525461967No Hit
GAGTAATGTGCTGAGCTTCTTACTACAAGGTTTTCCCAACAGCAATGATC14890.1131406522949924No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG14700.11169695021735315No Hit
CCTCAAAAGTATAAGATTAGCAGATACAAGACCTCCAAACTCTTCTTCAG14500.11017726381983813No Hit
GCTATGAGTAATGTGCTGAGCTTCTTACTACAAGGTTTTCCCAACAGCAA14480.11002529518008664No Hit
TCTTACTACAAGGTTTTCCCAACAGCAATGATCTGCTCAGGTTTGGCCAG14170.10766978126393836No Hit
GAAGAAGAGTTTGGAGGTCTTGTATCTGCTAATCTTATACTTTTGAGGAA14160.10759379694406263No Hit
GTATTAGGATTCAATGTGGGCAGGAGTGCTGGCGGGAGATCGACGGTCAG13950.10599812622667187No Hit
ATCTAACATTTTCCTTGGTGGGAACTGTTGATAGTGGGCATGAGACCTCC13610.10341465935089635No Hit
GCTTGATACTGGAACTGAAGAAGAGTTTGGAGGTCTTGTATCTGCTAATC13600.1033386750310206No Hit
GCATTACGTGTGCCTTGCCATTGTCTTATCCGCAAAAGATCGATCTCTGA13590.10326269071114486No Hit
CTTCACAAGCTTGCAAATTAATGCTTGTCTTTTCTTTAGATGTGTAAACT13550.10295875343164183No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA13500.1025788318322631No Hit
GTTTTGGAGTAGTTCCTCTTTGGTGGAAACAATCAGAGATCGATCTTTTG13420.1019709572732571No Hit
TCTAGATATCTTGCTAAAACTAGTTTACACATCTAAAGAAAAGACAAGCA13420.1019709572732571No Hit
GGTTTGGCCAGTTTATTTCTTCTACTTTACCAACGTTTGCAGTTTGTGAG13330.10128709839437534No Hit
ATGGTAGGTTGGGATAAAGACATATCTCGAAGAGTCAGTAGCAATTTGGG13210.10037528655586633No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)