FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B11a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_B11a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767459
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT401375.22985592715702No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG232613.030911097530943No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC195342.545282549295793No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC146361.907072560227973No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC116191.5139570973824008No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA116031.5118722954581287No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC106961.3936900863759496No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA101921.3280188257613763No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC84931.1066389214277246No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC70450.9179643472810926No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT58010.7558709976689308No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG57470.7488347911745122No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA55380.721602066038707No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT54810.7141749591834873No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA53680.6994510455933151No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA53330.6948905413839697No Hit
GTATATAGCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTT52890.6891573360922212No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG50940.6637488126401541No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT45600.5941685484175702No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA45510.5929958473351671No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG44760.5832233383151413No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTG44110.5747538304977855No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC41910.5460878040390431No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA39050.508821969642678No Hit
ACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGC37380.4870618495580872No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATT34430.4486233140793189No Hit
GTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGG34120.4445840103510416No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC33910.44184770782543437No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCG33400.4352024016918168No Hit
CATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATAT31880.4153967834112311No Hit
TAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGT31180.4062757749925403No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG27760.36171313386122256No Hit
AAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCT27420.35728292977214415No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC27310.35584962844920703No Hit
GTAGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT27100.35311332592359984No Hit
TACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACT26850.3498558229169245No Hit
GAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGC26630.3469892202710503No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA26500.3452953187075792No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC26200.34138631509956885No Hit
GAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGC26040.33930151317529667No Hit
GTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT25930.33786821185235955No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA25640.3340895083646162No Hit
AACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGA25330.3300502046363389No Hit
CTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTA25070.3266624015093966No Hit
ATGTATAGGTTATCATGCGATCTATTCAACTGTCGCCAGTTCATTGGTAC24450.3185837940528419No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG24440.3184534939325749No Hit
GAGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT23860.31089608695708826No Hit
CATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACTAG23480.30594468238694184No Hit
GGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACA23240.30281747950053356No Hit
CAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACA23150.3016447784181305No Hit
GTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGC23080.30073267757626143No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC22740.296302473487183No Hit
AATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACAT22720.296041873246649No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA22600.2944782718034449No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGA22000.2866602645874242No Hit
ATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCG21870.28496636302395306No Hit
AGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGAC21660.28223006049834587No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA21630.2818391601375448No Hit
GTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTT21470.2797543582132726No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTC21170.27584535460526227No Hit
AAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAAT20800.2710242501553829No Hit
GGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTAT18980.24730962826678687No Hit
CTGTAGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCG18700.2436612248993106No Hit
ACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTAT18060.23532201720222184No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA17330.22581010842273008No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA17030.22190110481471975No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA16950.22085870385258366No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA16750.21825270144724343No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAC16400.21369219723789806No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA16110.20991349375015472No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTAT15680.20431058857867326No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA15280.19909858376799283No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT15270.1989682836477258No Hit
GTTGAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT15260.1988379835274588No Hit
CTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATA15140.19727438208425466No Hit
ATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTC15050.19610168100185157No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG14880.19388657895731237No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA14120.18398376981701955No Hit
TAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATA14060.18320196909541747No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT13850.18046566656981025No Hit
CTGTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCG13530.17629606272126588No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG13490.17577486224019784No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG13230.17238705911325555No Hit
CATTATGTATAGGTTATCATGCGATCTATTCAACTGTCGCCAGTTCATTG13140.17121435803085247No Hit
CTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAAC13000.1693901563471143No Hit
CATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGC12700.165481152739104No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAG12600.16417815153643386No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC12350.1609206485297586No Hit
GTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAG11880.1547965428772091No Hit
TGATAACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAG11700.15245114071240287No Hit
CAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACC11530.1502360386678637No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA11250.14658763530038738No Hit
AAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACA11130.14502403385718324No Hit
GTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATT10970.14293923193291108No Hit
CTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTTCCCCTGCTTTTGC10630.1385090278438327No Hit
AATGTATATAGCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTAT10620.1383787277235657No Hit
GAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGT10610.1382484276032987No Hit
GCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG10590.13798782736276466No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGCTGTC10510.13694542640062857No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCA10310.13433942399528834No Hit
TGCAGACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTG10250.13355762327368628No Hit
AGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTA10240.13342732315341926No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGCTGTCT10220.13316672291288525No Hit
TTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCT10180.1326455224318172No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA10090.1314728213494141No Hit
CTTTTATTCTCTCCATATTGAATATAATTGACCTGCTTTTGCTCCTGTCT9940.12951831954540893No Hit
GAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTTCCCCTGCTTT9770.12730321750086976No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTT9640.1256093159373986No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCATGATAACCTATACAT9590.12495781533606355No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG9420.12274271329152436No Hit
GGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTAGAAAA9320.12143971208885425No Hit
TAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACT9320.12143971208885425No Hit
CATCCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTTCCCC9020.11753070848084393No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCCTGTC8900.1159671070376398No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT8890.11583680691737279No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT8880.11570650679710577No Hit
TCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGT8740.11388230511336762No Hit
CATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTT8710.11349140475256658No Hit
GTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACAT8560.11153690294856142No Hit
CTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTC8550.1114066028282944No Hit
GTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGTGT8530.1111460025877604No Hit
TAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACTAGTA8490.11062480210669234No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA8480.11049450198642533No Hit
CAATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAA8450.1101036016256243No Hit
CAGTTGAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCG8440.10997330150535728No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTCTCTCCATA8350.1088006004229542No Hit
CTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAG8180.10658549837841501No Hit
GTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC8170.10645519825814799No Hit
ATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATA8130.10593399777707996No Hit
ATGTATAGGTTATCATGCGAACAATTCAACTGTCGCCAGTTCATTGGTAC8030.10463099657440984No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA8010.10437039633387582No Hit
CCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATT7920.10319769525147272No Hit
CTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCA7790.10150379368800157No Hit
TGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGTG7790.10150379368800157No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)