FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1652321
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA218451.3220796685389824No Hit
GGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCA188241.139245945551742No Hit
CAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTT156270.9457605392656754No Hit
ATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTT147790.8944387924622395No Hit
TAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAG121300.7341188546293366No Hit
ATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCT120390.7286114501964207No Hit
AGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAA107200.6487843463830576No Hit
CCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACA100070.6056329248372441No Hit
TTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTT95640.5788221538066756No Hit
GCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGAT85150.5153357005085574No Hit
CTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTC82330.49826879885930153No Hit
TATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAAC81690.49439545947791014No Hit
ATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGAC80440.48683034349863014No Hit
ATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAA80310.486043571436785No Hit
ATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCA78630.47587605556063256No Hit
TATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCA78220.47339469751942875No Hit
CTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTG78030.4722447998905781No Hit
GTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTC74980.45378591690113484No Hit
GTTTTATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGC74480.45075987050942284No Hit
AGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCATAAAA70820.4286092109220908No Hit
GATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTA68620.4152946067985579No Hit
GCTTTATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGT67250.40700323968526697No Hit
GTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAG64320.3892706078298345No Hit
CTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAG62100.37583496185063314No Hit
ATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAAT61870.3744429805104456No Hit
GTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTC61460.37196162246924175No Hit
CAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAA61270.3708117248403912No Hit
CTCTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGT57780.3496899210262413No Hit
GGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTT56900.3443640793768281No Hit
AGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATC56040.33915927958308345No Hit
CTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAA50570.3060543320577539No Hit
GTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTT48820.29546316968676184No Hit
GCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTG45820.2773068913364897No Hit
AGCAGGGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCA45720.2767016820581473No Hit
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA45350.2744624077282804No Hit
AGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTT44760.2708916729860602No Hit
GCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAA42930.2598163431923942No Hit
CTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCT42920.25975582226455995No Hit
GTACTAAGGCACCATAAAAATAAAACAGATAAAATTGGTTTTAAAAATAG42820.25915061298621755No Hit
TCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTG41480.25104080865642936No Hit
CTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTC41300.249951431955413No Hit
AGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGA41290.24989091102757877No Hit
GACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTT41000.2481358041203858No Hit
GGTAAGACTTCTGTGGTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAG40950.24783319948121463No Hit
TCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCT40890.24747007391420917No Hit
GATTAATATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAG40170.24311256711014384No Hit
CTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTAATCCTACTACCACTA39850.24117589741944814No Hit
GATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAA39430.23863401845041007No Hit
ATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTAT38380.23227932102781482No Hit
GCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGAC38340.23203723731647785No Hit
TCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTAC38040.23022160948145062No Hit
GCACTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCA37500.22695347937840166No Hit
GTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATT36770.2225354516465021No Hit
CCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAAT36770.2225354516465021No Hit
ATCCCAGGAAGGACCCTGGGATCATGACCTGAGCCAAAGGCAGACACTAA35530.2150308565950563No Hit
ATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCA35260.21339679154353178No Hit
GATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAA34940.2114601218528361No Hit
TAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTC34780.21049178700748825No Hit
TTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATC34080.20625532205909144No Hit
AATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGG33690.20389500587355605No Hit
CACCCAGGCATCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTT33600.20335031752304789No Hit
CTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGT33570.20316875473954518No Hit
GGTCATGATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCC33190.20086895948184402No Hit
CTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTA33160.20068739669834132No Hit
GAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTG33140.2005663548426728No Hit
GAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTC32850.19881124793547986No Hit
GCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGC32620.1974192665952923No Hit
ATCATGACCTGAGCCAAAGGCAGACACTAACCGACCAAGCCACCTAGGCA32480.19657197360561296No Hit
ATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGC32440.19632988989427602No Hit
GTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGCTCTTT32140.19451426205924877No Hit
GGATAAGTAGGCTTCCCACTGAGCAGGGAGCTCCATGCAGTGCTCAATCC31600.1912461319561998No Hit
GCTAATAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTC31490.19058040175002314No Hit
CAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAG31360.18979362968817803No Hit
CCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGG31120.18834112742015624No Hit
GATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACT30790.18634393680162634No Hit
GTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATCT29980.18144174164705285No Hit
CAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATT29380.17781048597699842No Hit
AGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGAC29260.17708423484298755No Hit
GTTAGTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGA28780.174179230306944No Hit
ATCCCAGGACGCAGGGGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCA28640.17333193731726462No Hit
CACTTAGCCAGTGGACACACCCAGGCATCCCTGTTATTAGCTATTTTTAA28540.17272672803892222No Hit
ATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGACGACAAACTG27970.16927703515237053No Hit
GTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATT27930.16903495144103356No Hit
GAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGC27680.16752192824517753No Hit
AATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAAC27460.16619046783282426No Hit
ATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAG27430.16600890504932153No Hit
ACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTC26480.16025941690506867No Hit
GCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCT25690.1554782636061637No Hit
GGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGACCCCTGCGTCCTGGGATT25560.15469149154431855No Hit
CCTCTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTAATCCTACTACCA25450.15402576133814191No Hit
TCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTTTTATTTTTAT25280.15299690556495985No Hit
ATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAA25190.15245221721445168No Hit
CTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCC24800.1500919010289163No Hit
GAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTCTC24680.14936564989490542No Hit
ACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGG24520.14839731504955755No Hit
CTATTCACTTTCTCTAATCCTACTACCACTATCTTATTATAGTTACTGTT24490.14821575226605482No Hit
GTTATTAGCTATTTTTAAAACCAATTTTATCTGTTTTATTTTTATGGTGC24290.14700533370937005No Hit
GCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCAGGTCATGATC24130.1460369988640222No Hit
GAGCAGGGAGCTCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAA23560.14258730597747046No Hit
CCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTT23510.14228470133829926No Hit
GTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACT23500.14222418041046503No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC23430.14180053391562536No Hit
CATTGAGTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATTCA23400.14161897113212263No Hit
TTATTTGACAGAGAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAG23380.14149792927645416No Hit
GGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCAGTGGACACACCCAGG22960.13895605030741603No Hit
GAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCA22900.1385929247404106No Hit
CTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGA22850.1382903201012394No Hit
TAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAA22660.13714042247238883No Hit
TCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGA22600.1367772969053834No Hit
AGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAA22550.13647469226621217No Hit
TTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGC22380.1354458364930301No Hit
ACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAG22270.13478010628685347No Hit
CTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTT22150.13405385515284257No Hit
ACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCT22030.13332760401883167No Hit
CAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTTAGT21840.13217770638998114No Hit
CCCTTATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATT21810.1319961436064784No Hit
GGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAA21610.1307857250497936No Hit
GGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGA21350.12921218092610334No Hit
AGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTA21220.1284254088642582No Hit
GTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGATTGA20750.12558092525604891No Hit
GAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGA20750.12558092525604891No Hit
AACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCA20690.12521779968904348No Hit
AACTAGGATGCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGT20560.12443102762719833No Hit
GGCAAGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTA20450.1237652974210217No Hit
GGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTT20380.12334165092618202No Hit
CAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACTAGGAT20370.12328112999834778No Hit
GCTCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAACTGAGCTGA20150.12194966958599449No Hit
ACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGATTTTAT20030.1212234184519836No Hit
CTTCTGTTTCCCCTGCTTGTGCGCTCTTTCTCTCTCTCTGTCAAATAAAA19950.12073925102930969No Hit
AAATAAAACAGATAAAATTGGTTTTAAAAATAGCTAATAACAGGGATGCC19880.12031560453446999No Hit
GGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCAGGGAG19760.11958935340045912No Hit
AAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTC19700.11922622783345367No Hit
GAACAAAAGATTCATTATTTCAAGATTTTATTTGACAGAGAGAGAGAAAG19490.11795528834893462No Hit
CCCTGGGATCATGACCTGAGCCAAAGGCAGACACTAACCGACCAAGCCAC19480.11789476742110037No Hit
AGCGAAAGCAGGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTA19190.11613966051390741No Hit
GCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCATGACCTGAG19030.11517132566855957No Hit
GAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAATGA18800.11377934432837203No Hit
GTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAAT18480.11184267463767635No Hit
AACATGCACAACTGAATTTGAAGACGACAAACTGAGGACCAAATGGAAGA18260.11051121422532303No Hit
AATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTTAGTCA18250.1104506932974888No Hit
GATGAAAAGGTAAGACTTCTGTGGTTTTAGTCAGCCATACTGTGAGGAAA18090.10948235845214095No Hit
ATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCATGACCTGAGCCAAA18020.10905871195730128No Hit
CCATAAAAATAAAACAGATAAAATTGGTTTTAAAAATAGCTAATAACAGG18020.10905871195730128No Hit
CCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATCTTGCCAG18000.10893767010163279No Hit
CCACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTG17870.10815089803978767No Hit
GAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACT17770.10754568876144527No Hit
GGCAGACACTAACCGACCAAGCCACCTAGGCACCCCAAGATTAATCCATT17750.10742464690577677No Hit
GAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAG17700.10712204226660559No Hit
AAAGCAGGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAAT17640.10675891669960014No Hit
GGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGT17610.10657735391609742No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC17520.10603266556558925No Hit
AAAGCAGGGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAG17430.10548797721508109No Hit
TCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCT17060.10324870288521419No Hit
GGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATA17050.10318818195737996No Hit
CTCTAATCCTACTACCACTATCTTATTATAGTTACTGTTACCTCTCCCCT16990.10282505639037452No Hit
GGTTTTTGTGAATGCTTTATCTAGATATTAATCATTATTTCTTCCATTTG16950.10258297267903756No Hit
CCTAGGCACCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAA16910.10234088896770059No Hit
GCTCTCCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGAC16700.10106994948318154No Hit
GACACTAACCGACCAAGCCACCTAGGCACCCCAAGATTAATCCATTTTAG16660.10082786577184458No Hit
TTAATATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGC16640.1007068239161761No Hit
GCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGA16550.10016213556566793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)