FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4098701
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAAAGCGCGCGCTATGACGGGCTACCTCCCACTAGGCCAGGACTATT318610.7773438462576314No Hit
ACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCGATATCGCGCAGGC217300.5301679727308726No Hit
TGCCTGCGCCGTGCGCACCATTTACGGCGATGACCCCTTGGATGCACAGC213440.5207503548075354No Hit
GAACTATATTTCCGATGTGGACGTCATTTTCGTCGGCAGCGAGGCAACCC183470.44762962704525167No Hit
ACCTACCGCACTTTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCAC177590.433283618395194No Hit
ACTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAAAGCG172880.42179217269081104No Hit
ATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATGACTGCCAG156670.3822430570075739No Hit
CCATTTACGGCGATGACCCCTTGGATGCACAGCTGGCAGTCATGGCGATG149580.36494489351626286No Hit
CTTTAAGGAGCGCCTGAAATTCCCACGTCTCCGCCCACCGCTTGTAGTAG147770.3605288602413301No Hit
CTATATTTCCGATGTGGACGTCATTTTCGTCGGCAGCGAGGCAACCCCTC127670.3114889327130718No Hit
ATTTACGGCGATGACCCCTTGGATGCACAGCTGGCAGTCATGGCGATGGG126680.30907353329750087No Hit
CCTTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGACGTGGGAA125250.30558462303056505No Hit
GCCTACTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAA123670.30172974315520945No Hit
CTCATAGGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCG122450.2987531903400614No Hit
ATATTTCCGATGTGGACGTCATTTTCGTCGGCAGCGAGGCAACCCCTCGA120130.29309286039650123No Hit
GTACAGGCGATGCGCCGCCGGGTGCTGGCCAATGTTCCCGCAGAGCTCAA115680.28223576201337935No Hit
GGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCA109290.26664545669469425No Hit
GCGCAGGCAAGGGAGGCGGTTAAGGCGGCGTCGGCAAGCGCGGTGGTCAG109110.26620629316459044No Hit
GTGCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCT106560.2599848098214532No Hit
CGCCTAGCTTTAGCTCGCGGTGCTTGAGCTCTGCGGGAACATTGGCCAGC103790.2532265710526335No Hit
GCTTTAGCTCGCGGTGCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGG103510.2525434277835832No Hit
TCCAAGGGGTCATCGCCGTAAATGGTGCGCACGGCGCAGGCAAGGGAGGC102440.24993284457685497No Hit
GAAATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATGACTGC95740.23358620206743552No Hit
CCCCTTGGATGCACAGCTGGCAGTCATGGCGATGGGCAAGTGCGGCGCAG95570.23317143651122638No Hit
GCGTCTCATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAAT94960.23168316010365236No Hit
ATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCAGCGCGAGTCT94820.23134158846912717No Hit
TCCCTTGCCTGCGCCGTGCGCACCATTTACGGCGATGACCCCTTGGATGC92880.2266083815335639No Hit
GTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAGGAGCG90960.22192397054579No Hit
ATTCGATATCGCGCAGGCCGCCCACGCCTAGCTTTAGCTCGCGGTGCTTG90660.22119203132895032No Hit
GTTTAATCGCATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGC90090.21980134681695496No Hit
CATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCGATA88540.21601966086328325No Hit
CCGTAAATGGTGCGCACGGCGCAGGCAAGGGAGGCGGTTAAGGCGGCGTC86050.20994456536351397No Hit
CGCCTGTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCA85220.2079195335302575No Hit
GTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCATCGGG82490.20125888665701647No Hit
TCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTG80850.19725761893829288No Hit
AAATAGTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAG80690.1968672513559784No Hit
GAGCTGGACCGCAAATTCGATATCGCGCAGGCCGCCCACGCCTAGCTTTA79780.19464703573156472No Hit
TCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAATCAG78690.19198765657704722No Hit
CGTAAGGGCCAGACCAAGCTGCAGCCGGAGGTAGGATTTCGCCAGATTAC76760.18727884761537864No Hit
GTCCATGGTCGCTCTGATGAGACGCTGCGCGCGCTTTCCACGGTGGAGGC75540.1843022948002306No Hit
CCTGTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCATC74640.1821064771497116No Hit
GGTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGA74500.1817649055151864No Hit
GTCATGGCGATGGGCAAGTGCGGCGCAGGCGAGCTGAACTATATTTCCGA72760.17751965805751627No Hit
GTCATAGCGCGCGCTTTAAGGAGCGCCTGAAATTCCCACGTCTCCGCCCA72400.17664133099730867No Hit
GGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCAGCGCG70380.17171294027058817No Hit
GCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAATCAGCGCCTCC69280.169029163142176No Hit
ACCGTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGACCAATTG69240.1689315712465974No Hit
CTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAAAGCGC69170.16876078542933481No Hit
AACTCATAGGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCC68270.16656496777881577No Hit
GCGCACGACCTACCGCACTTTGATGATGCGCATTGCCGCCTGCGATGTGG67920.1657110386925028No Hit
CTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCA67780.16536946705797764No Hit
CGCTATGACGGGCTACCTCCCACTAGGCCAGGACTATTTGGACCAGATTC67670.16510108934513643No Hit
CTTTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCACCTTCCATTCA67140.16380799672871968No Hit
ATGTACAGGCGATGCGCCGCCGGGTGCTGGCCAATGTTCCCGCAGAGCTC65460.15970913711441748No Hit
CTATGACGGGCTACCTCCCACTAGGCCAGGACTATTTGGACCAGATTCGC65060.15873321815863123No Hit
AGCTAGGCGTGGGCGGCCTGCGCGATATCGAATTTGCGGTCCAGCTCTTG64800.15809887083737018No Hit
GCTGGCAGTCATGGCGATGGGCAAGTGCGGCGCAGGCGAGCTGAACTATA63790.15563467547400994No Hit
ACCTCCCACTAGGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGG63180.15414639906643593No Hit
GGTGCGCACCTTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGA62710.1529996942933871No Hit
GTCCACACCATCGGGCGAATCTGGTCCAAATAGTCCTGGCCTAGTGGGAG61910.15104785638181462No Hit
GACGTACCCCGCTGAAATCAGCGCCTCCAGCGCCTCCACCGTGGAAAGCG61350.1496815698437139No Hit
CACTAGGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCA60810.14836407925340248No Hit
TTTCCGATGTGGACGTCATTTTCGTCGGCAGCGAGGCAACCCCTCGAGCC60660.14799810964498264No Hit
ATATCGAATTTGCGGTCCAGCTCTTGCAATTGGTCCATGGTCGCTCTGAT60590.14782732382772004No Hit
AAACAAGGAAGTGATGGGACCGCAGAGATGAGACCCCCAGGGATGAGATG60430.14743695624540556No Hit
CCTTCCATTCACGTAAGGGCCAGACCAAGCTGCAGCCGGAGGTAGGATTT59880.14609506768119948No Hit
ATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTGGTGCGCACCTTG59200.14443600545636287No Hit
GCTCAAGCACCGCGAGCTAAAGCTAGGCGTGGGCGGCCTGCGCGATATCG59040.1440456378740484No Hit
GTAGTAGGCCACGTGGGATTCCAAGGTGCGCACCAATGCGCCCGACTTTC57450.1401663600247981No Hit
GGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAA56990.13904405322564395No Hit
GCCTTAACCGCCTCCCTTGCCTGCGCCGTGCGCACCATTTACGGCGATGA54970.13411566249892345No Hit
GGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAA54820.13374969289050362No Hit
AGCTAAAGCTAGGCGTGGGCGGCCTGCGCGATATCGAATTTGCGGTCCAG54680.13340812125597842No Hit
TGGATGCACAGCTGGCAGTCATGGCGATGGGCAAGTGCGGCGCAGGCGAG53710.13104151778819678No Hit
ACATTGGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCAACAAAAGA53430.13035837451914645No Hit
GGCAAGGGAGGCGGTTAAGGCGGCGTCGGCAAGCGCGGTGGTCAGCGCCG52740.12867491432041517No Hit
GTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGAT52730.12865051634652053No Hit
CCCTCGAGCCACGCGGCTGGCGGCGGAGTTTAATCGCATTGGATCCACCA51100.12467364660169161No Hit
TCTCATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCG50240.1225754208467512No Hit
CATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGG50210.12250222692506724No Hit
GGTCCAAATAGTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCT50170.1224046350294886No Hit
GCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGATGCGCCGCCG50150.1223558390816993No Hit
AGCGAAAGCAGGCGCAGAAACTCATAGGCTTCAATCAGCTGGGTGCCGTC50030.12206306339496342No Hit
CGCCTCCACCGTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGA50000.12198986947327946No Hit
ATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGT49550.12089196064801995No Hit
ATGGTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGC49430.12059918496128408No Hit
GTGCGGCGCAGGCGAGCTGAACTATATTTCCGATGTGGACGTCATTTTCG48980.11950127613602456No Hit
TCGGAAATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATGAC48600.11857415312802765No Hit
GCATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAG47040.11476806920046131No Hit
GCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAAT46860.1143289056703575No Hit
GCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCTGT46760.11408492593141095No Hit
GTTCCCGCAGAGCTCAAGCACCGCGAGCTAAAGCTAGGCGTGGGCGGCCT46630.11376775227078043No Hit
GGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCAACAAAAGACTCGC46280.11291382318446748No Hit
TTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGACGTGGGAATT45820.1117915163853133No Hit
CTGCGATGCCGTCCACACCATCGGGCGAATCTGGTCCAAATAGTCCTGGC45730.1115719346202614No Hit
TTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCACCTTCCATTCACG45410.1107911994556324No Hit
ACAAAAGACTCGCGCTGCGATGCCGTCCACACCATCGGGCGAATCTGGTC44730.1091321372307958No Hit
GTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGACCAATTGCAA44440.10842459598785079No Hit
GCTGAAATCAGCGCCTCCAGCGCCTCCACCGTGGAAAGCGCGCGCAGCGT44080.10754626892764316No Hit
CGCCATGACTGCCAGCTGTGCATCCAAGGGGTCATCGCCGTAAATGGTGC43240.10549683912049207No Hit
GACCAATTGCAAGAGCTGGACCGCAAATTCGATATCGCGCAGGCCGCCCA43070.10508207356428292No Hit
GCGAAAGCAGGCGCAGAAACTCATAGGCTTCAATCAGCTGGGTGCCGTCC42660.10408175663460204No Hit
CCCCTCGAGCCACGCGGCTGGCGGCGGAGTTTAATCGCATTGGATCCACC42570.10386217486955014No Hit
CTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTGG42540.10378898094786616No Hit
ACTGCCAGCTGTGCATCCAAGGGGTCATCGCCGTAAATGGTGCGCACGGC42310.10322782754828908No Hit
GGCAAGTGCGGCGCAGGCGAGCTGAACTATATTTCCGATGTGGACGTCAT42020.10252028630534406No Hit
ATGACGGGCTACCTCCCACTAGGCCAGGACTATTTGGACCAGATTCGCCC41940.10232510251418682No Hit
CATCGGAAATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATG41720.10178834708850439No Hit
AGGATGTACAGGCGATGCGCCGCCGGGTGCTGGCCAATGTTCCCGCAGAG41650.10161756127124179No Hit
CCCTTGCCTGCGCCGTGCGCACCATTTACGGCGATGACCCCTTGGATGCA41510.1012759896367166No Hit
ATATCGCGCAGGCCGCCCACGCCTAGCTTTAGCTCGCGGTGCTTGAGCTC41110.10030007068093037No Hit
CCTCCAGCGCCTCCACCGTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGA41010.10005609094198382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)