FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228056
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTGAGAGTGGAAT88430.7200811689369214No Hit
GTTCACAGCTGCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACT75340.6134899385695767No Hit
CAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTT58970.48018982847687725No Hit
CCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACAGG58940.4799455399428039No Hit
CCCTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGCAGTATC49910.40641469118672113No Hit
CTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGG43910.3575569843720482No Hit
ACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAG43540.35454409245181No Hit
GGCTAGGAACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCAC39070.31814510087487863No Hit
ATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACC38790.3158650745568606No Hit
TCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGGGCAGTGACCGA38590.31423648432970486No Hit
CCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCC37670.30674496928478834No Hit
CTCCAGGATTCCCACTCAGCAGCAGGGCAGTGACCGACTTTTACTCCCAG36770.2994163132625874No Hit
GCTGTGAACAGTGGGCTCCACCTGACACCCACACCCTCAGGGAGGGACCT36600.298032011569505No Hit
CCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACGG34320.27946608297992925No Hit
GCCCTGGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGTAAAAG33630.2738474466962419No Hit
GCATAGCCCTGGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGT33300.27116027282143484No Hit
GTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATTCACCA32080.26122587243578466No Hit
AATTCATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAACAAC32020.26073729536763796No Hit
GCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGA30450.2479528620844652No Hit
CATCCATACTTTGGAGAAAGAGCAGAGCCCACTGTGGCATCACCCCACAG29910.24355566847114465No Hit
AGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGA29390.23932133388053964No Hit
GGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTAT29270.23834417974424618No Hit
CTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCT29110.23704130756252156No Hit
CACCAGGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCTGGTCT28630.23313269101734774No Hit
GACATATGCCTTCCAGCATATTCTCGTCTCCAGCACACAAATTCATTCCA28060.22849120886995383No Hit
TCCCAATCCTGGTTGCCCGGGCACAGAGTAGTTGTTGAACATGGATGAAT27730.2258040349951468No Hit
CCAGGGGACTTGGCTGCAAACCCAACAGATGATGGAGAAGACATATGCCT27350.22270971356355085No Hit
TGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAGAGCAG27120.22083683480232172No Hit
GGATTGGGAACCCAGCTTTTTAGTTCAATTCACCAAATGTTTGTTGTCAC26720.2175796543480102No Hit
CTCCCACCTCTCCCCACCACCCTCCTAGCGCAGCACTCCAGGATTCCCAC25610.2085409785872957No Hit
TCTTCAGGCTGGGAGTAAAAGTCGGTCACTGCCCTGCTGCTGAGTGGGAA25430.2070752473828555No Hit
GACATATGCCTTCCGGCATATTCTCGTCTCCAGCACACAAATTCATTCCA25290.20593523422384646No Hit
TTTTAGTTCAATTCACCAAATGTTTGTTGTCACAGAGCCTGTGCAGCTGT25170.204958080087553No Hit
CTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATTC24310.19795514211078322No Hit
GGGCAGACTGAGCTCCAGGGGACTTGGCTGCAAACCCAACAGATGATGGA23870.19437224361104052No Hit
GGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCC23480.1911964926680868No Hit
CTTTTACTCCCAGCCTGAAGATCTGCATGAGAACCAATGCACACCAGGGC23270.18948647292957324No Hit
AATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGTGTGCTGGAGACGAG22550.18362354811181247No Hit
GAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCA22420.18256496446416123No Hit
GCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGG22220.18093637423700548No Hit
GGCACAGAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTGAGAG21960.178819206941703No Hit
AGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCAT21920.17849348889627184No Hit
CAGCTGCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAA21890.1782492003621985No Hit
GAACTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGGGCACAGAGTAGTT21590.17580631502146482No Hit
GATGAGGAATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGAGATGGTG21410.17434058381702464No Hit
CACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTG21360.1739334362602357No Hit
CACAGAGCCTGTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACACC20670.1683147999765483No Hit
CCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAG20450.16652335072667696No Hit
GTGGGGAGGCATCCATACTTTGGAGAAAGAGCAGAGCCCACTGTGGCATC20210.16456904245409004No Hit
GCATATGTCTTCTCCATCATCTGTTGGGTTTGCAGCCAAGTCCCCTGGAG20160.1641618948973011No Hit
CACAAATTCATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAA20030.16310331124964986No Hit
GGTTTCATTCATCCATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGA19910.1621261571133564No Hit
AGATCACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCT19800.16123043248842073No Hit
GGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCTGGTCTCACCA19770.16098614395434738No Hit
GCATATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGG19540.15911326519311822No Hit
GCCTGAAGATCTGCATGAGAACCAATGCACACCAGGGCTATGCTGCCTCC19190.15626323229559563No Hit
CTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCA18650.15186603868227508No Hit
CTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAATA18630.15170317965955948No Hit
GCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAG18550.15105174356869722No Hit
GAGTAAAAGTCGGTCACTGCCCTGCTGCTGAGTGGGAATCCTGGAGTGCT18510.15072602552326603No Hit
TCTGTGGGGTGATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATGGAT18500.15064459601190824No Hit
GAGGTGGGAGAGGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCA18390.1497488713869726No Hit
GACCAGGCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCTGGTGT18380.1496674418756148No Hit
GTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTG17950.1461659728872299No Hit
ATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGGTTTC17860.1454331072850098No Hit
CTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACA17660.14380451705785405No Hit
AGGTGGGAGAGGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCAA17620.1434787990124229No Hit
CCCGGGCACAGAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTG17560.14299022194427616No Hit
GTCACAGAGCCTGTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACA17500.14250164487612943No Hit
CCCTCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGGGCAGTGAC17340.1411987726944048No Hit
CCCCACCACCCTCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGG17330.14111734318304703No Hit
GGAGATCACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATC17280.14071019562625808No Hit
CATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGG17180.1398959005126802No Hit
ACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACG17140.13957018246724906No Hit
AGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACCAGGCCCTGGGGACCAC17090.13916303491046011No Hit
GCCCACTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCA17010.13851159881959782No Hit
ATCCTGGAGTGCTGCGCTAGGAGGGTGGTGGGGAGAGGTGGGAGAGGGCA16880.13745301517194658No Hit
CTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGCAGTATCTG16840.1371272971265154No Hit
GCTGAGGTCCCTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCA16370.13330011009269938No Hit
ACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATT16350.13313725106998378No Hit
CTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAG16160.13159009035418578No Hit
GCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGGTGTGGG16090.13102008377468127No Hit
GTATGGATGCCTCCCCACTCCCCGAGTCTCAGCATGTGCTGGGCACAGCC16040.13061293621789233No Hit
ACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTC15710.12792576234308534No Hit
AGCGAAAGCAGGCTAGGAACCTAGGTCTCTGCACTCCAGAGCATGGGCTG15600.12703003771814966No Hit
CATACATGCAGCCTCAGTTTTGGTGATGTTATTTTTCAAGCTGGGTGGCA15500.1262157426045718No Hit
GACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAGAGCAGAGCCCA15490.126134313093214No Hit
GTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACACCCTCAGGGAGG15460.1258900245591406No Hit
CTGGAGAGATGAGGAATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGA15450.12580859504778283No Hit
GTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTT15360.12507572944556272No Hit
GTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACGGGAGCAAG15330.12483144091148939No Hit
ATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATGGATGCCTCCCCACT15170.12352856872976477No Hit
GAGCAGAGCCCACTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGG15090.12287713263890246No Hit
ATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGGT15030.12238855557075573No Hit
GTATCTGTGGGGTGATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATG14950.12173711947989342No Hit
ATGCTGAGGTCCCTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTT14710.1197828112073065No Hit
GCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATC14670.11945709316187536No Hit
GTTGAACATGGATGAATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGT14620.11904994560508642No Hit
GATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGG14470.1178285029347196No Hit
CTCAGCAGCAGGGCAGTGACCGACTTTTACTCCCAGCCTGAAGATCTGCA14360.11693277830978391No Hit
GGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGTAAAAGTCGGT14350.11685134879842612No Hit
ATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCT14330.11668848977571056No Hit
CTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCAAT14120.11497847003719701No Hit
GGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCAAACCCAACAGA14110.11489704052583923No Hit
GCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCTGGTGTGCATTG14050.1144084634576925No Hit
CCCACACCCTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGC14030.11424560443497692No Hit
GGAGCAAGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACCAGGCCCTGGG13970.11375702736683019No Hit
ACTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAAT13740.11188414860560106No Hit
TCCATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCA13710.11163986007152768No Hit
GAACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTG13670.11131414202609653No Hit
GGTGAGACCAGGCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCT13660.11123271251473875No Hit
GAATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGTGTGCTGGAGACGA13570.11049984691251864No Hit
CACCAAATGTTTGTTGTCACAGAGCCTGTGCAGCTGTGAACAGTGGGCTC13550.11033698788980306No Hit
ATGCACACCAGGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCT13310.10838267961721616No Hit
CCCAACAGATGATGGAGAAGACATATGCCTTCCAGCATATTCTCGTCTCC13300.10830125010585837No Hit
ATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAACAACTACTC13270.10805696157178499No Hit
CCCCAGGGCCTGGTCTCACCACCATCTCCGCTTCCACCACAGACTTGCTC13220.10764981401499606No Hit
TCTGTTGGGTTTGCAGCCAAGTCCCCTGGAGCTCAGTCTGCCCTCTGCCC13180.10732409596956492No Hit
ATATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGGTT13160.10716123694684933No Hit
GGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACAGGCTCTGT13050.10626551232191365No Hit
GCTGCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGC12860.10471835160611569No Hit
GGAGAAGACATATGCCTTCCAGCATATTCTCGTCTCCAGCACACAAATTC12690.10333404991303327No Hit
CTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGGGCACAGAGTAGTTGTT12560.10227546626538203No Hit
CAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAG12420.101135453106373No Hit
CTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGGTGTG12340.10048401701551068No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)