FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8700147
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGACTCAG675250.7761363112600281No Hit
GTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCAAAATTTTGAATTC534980.61490915038562No Hit
AAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCAC431840.4963594293291826No Hit
GTTAAATACTATGGCTTAAAACATTAAGCCACCAATACCAAGATTTATTG353020.4057632589426362No Hit
GTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGCGCGAGCAA331040.38049931800002923No Hit
GTATATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGAC311870.35846520754189554No Hit
GAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCA293730.3376149851261134No Hit
CAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGGCTCCCTGCTGAGC278550.32016700407475873No Hit
ATATTATTGCTTCCACTATCAGGATTATTACCACCACCAAAAGGTGGCAT255980.2942249136709989No Hit
CCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAGAA254740.2927996503967117No Hit
CAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGCCATAGTATTTAAC254670.29271919198606644No Hit
GTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCA253040.2908456604238986No Hit
GTATTACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATT252200.28988015949615564No Hit
ATGTAATACCGATACATTTTAACTTGGCCTAAGGGATTTTTAAAATTATG245520.2822021283088665No Hit
ATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTCAGCTC242920.2792136730563288No Hit
GTGAAAGATAGCCCATAGTACCTACTAGTTCCTACTCTTATTTCTCAGTC241990.27814472560061343No Hit
CACTTATTTAGTCCTCAAATATGTCTAAGAAATAGGTACTGTTCTTTTTC241420.27748956425678784No Hit
ACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGA239390.2751562703480757No Hit
ATAATAACTAGGATGCAGTCAGCTACACTCAATTCTCTAGTTAATATACT223810.25724852695017686No Hit
GCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAA214290.24630618310242347No Hit
TACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAGGATTATTACCAC212020.24369703178578478No Hit
TTATTTATGTATGCAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGC209890.2412487972904366No Hit
GTACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATG206660.23753621634209168No Hit
GTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGAATTCAAAA205440.2361339411851317No Hit
GTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAA204930.23554774419328778No Hit
GTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAGTTGAT200620.2305938049092734No Hit
CATAAATAATGAAACTACCTAAGTGCATAGCTAATAGTTCTATTTAAGGA200360.23029495938401962No Hit
GTACTATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTCTAACT197900.22746742095277242No Hit
CACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTT196090.22538699633465964No Hit
GTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTTCC195540.22475482310816128No Hit
GTATGATGATACAAGTATGCCTCATTGGATAAAAGCCACTAGCCCCATTC181730.2088815280937207No Hit
AAATAGTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCCAAAAA178100.20470918479883154No Hit
TCATCATACTGCTCCCATGGAAATATGTAATACCGATACATTTTAACTTG177800.20436436303892339No Hit
GTATATTAACTAGAGAATTGAGTGTAGCTGACTGCATCCTAGTTATTATT177460.20397356504436073No Hit
CTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCC177080.2035367908151437No Hit
ATCCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAG170860.19638748632638045No Hit
GGATTATTACCACCACCAAAAGGTGGCATTGATGACCAGAAGAATTTGGA169780.19514612799071096No Hit
AGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAA167460.19247950638075426No Hit
AGTTATTATTATCCAACAGTGACTTTTGTTCCTTATTGGTTTAATTTGCA165560.19029563523466902No Hit
GTGTATAATGATAAAATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCA164870.18950254518688017No Hit
TATTCATACAGATGATGTGCCAAATCTCACAGAATATTTGGCAAAACACT164320.1888703719603818No Hit
ACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTGGCTTT163720.18818072844056544No Hit
ATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTCTAACTCAGGT159190.18297391986595168No Hit
ATACAGATGATGTGCCAAATCTCACAGAATATTTGGCAAAACACTCAGTG156760.18018086361069532No Hit
AAAATAAACTCACTTTAGTTAAATCTGCAAAAACCAGCATTAACCACTGG154660.17776711129133796No Hit
GAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTT154240.1772843608274665No Hit
CCTTAAGACTCTTCATCTTTAAAAATTTCATTAAATTTCGGGGCGCCTGG152690.17550278173460745No Hit
TTTCAAGGCTGATGTGCCAACAAAGTATATTAACTAGAGAATTGAGTGTA152000.17470969168681863No Hit
GTATCTGACACTTTGTATCCACTGCTTTGACTATATTCTCTGTGTATTCT151340.1739510838150206No Hit
ACATTACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTG151020.1735832739377852No Hit
GCTTTGTACCCAGTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTG150890.1734338511751583No Hit
GTTTCTAATCTTATGATGCAAATTAAACCAATAAGGAACAAAAGTCACTG150200.17264076112736945No Hit
GTTTTAAGCCATAGTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCA145820.16760636343270982No Hit
GTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATAT144910.16656040409432163No Hit
GTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGT142940.16429607453759115No Hit
ACTATTAGCTATGCACTTAGGTAGTTTCATTATTTATGTATGCAATAAAT142820.16415814583362787No Hit
AGTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGAATTCAAA141300.16241104891675967No Hit
CATTACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTGG140950.16200875686353347No Hit
GCATTAACCACTGGCTTTCTCAAGGCACCATCAGTCACAAAGAAACAGTT139850.16074441041053672No Hit
GTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAG139340.16015821341869282No Hit
GTTTAATTTGGTCCCGGCAAAATTTTGAATTCTGGGTATTTCAAGGCTGA137790.15837663432583382No Hit
GTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAGGA137360.15788238980329872No Hit
CCCTATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAA136940.15739963933942724No Hit
CCATAGTACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTA136320.15668700770228366No Hit
ACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTC135350.1555720840119138No Hit
GTACTGAAGTGATAAAATTAAAATAAACTAAGAGCCTGGAAATCTAGGAA135180.1553766850146325No Hit
CTTCAGTACATAATTTTAAAAATCCCTTAGGCCAAGTTAAAATGTATCGG132860.1527100634046758No Hit
TCCCAGGACCCTGAGCCAAAGGAGATGCTCAACCATCTGAGCCACCCAGG132810.15265259311135776No Hit
CAATAATATTTAAGCACTTTTGGTATGCCACATACACTTATTTAGTCCTC132290.15205490206085023No Hit
GCCTTGAAATACCCAGAATTCAAAATTTTGCCGGGACCAAATTAAACATC131720.15139974071702467No Hit
ATATCAAATAGTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCC131270.15088250807716236No Hit
CACTTAGGTAGTTTCATTATTTATGTATGCAATAAATCTTGGTATTGGTG129440.14877909534172237No Hit
CTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAAGAAAAAAGTA125250.14396308476167127No Hit
GATTTATTGCATACATAAATAATGAAACTACCTAAGTGCATAGCTAATAG125210.14391710852701683No Hit
GTTAAAATGTATCGGTATTACATATTTCCATGGGAGCAGTATGATGATAC123830.14233092843143916No Hit
CTACTAAACTCTTTTCCTATAAGGAACTTGGAAGATTCACATTTCTTCAC123230.14164128491162276No Hit
GCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAATGCCAC122820.14117002850641489No Hit
GGACATGGGAGCACAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGG121910.14012406916802672No Hit
GAGAATACACAGAGAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATA118550.1362620654570549No Hit
GTTAAGCACCTGCCTTCAGCTCAGGGCTTGATCCGGAGTCCCATATCTGG118340.13602069022511917No Hit
GTACAAAGCACTGTGAAGAATATAAAAGAAAAAAGTAGTATTTGTAAATT117300.1348253081241041No Hit
GTCTTAAGGGATAAAATTAGTTTAAAGGCTTTACAAATAAATTAATAAAC114770.13191731128221168No Hit
CTTATTTAGTCCTCAAATATGTCTAAGAAATAGGTACTGTTCTTTTTCCA114710.13184834693023No Hit
ATTATACACAATTCCATCTCAGAATGCATCCTGTTTATTAATTTATTTGT114120.13117019746907724No Hit
CCATTATTGTGCTCCCATGTCCCCTGCTGCTGTGCTTGCTCGCGCTCTTG113400.13034262524529758No Hit
ATCTGACACTTTGTATCCACTGCTTTGACTATATTCTCTGTGTATTCTCC112910.12977941637078086No Hit
ATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAAGCAGTGGATACA112580.12940011243488184No Hit
ACCTATTTCTTAGACATATTTGAGGACTAAATAAGTGTATGTGGCATACC112320.1291012669096281No Hit
ACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGA111970.12869897485640186No Hit
ATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAAT110980.12756106304870482No Hit
GAATATAACTGAAGCAAACCTGAGTTAGAAGAATGGAACAAAAATTTTCT110390.12688291358755202No Hit
AGTAGGTACTATGGGCTATCTTTCACTGAGTGTTTTGCCAAATATTCTGT109410.12575649583851858No Hit
GCTATGCACTTAGGTAGTTTCATTATTTATGTATGCAATAAATCTTGGTA109320.12565304931054613No Hit
GCATTGTACTATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTC107750.12384848210035991No Hit
GTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCCAAAAAATGAC107350.12338871975381566No Hit
GATAAAATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTGGG107180.12319332075653433No Hit
CTTTTGGTATGCCACATACACTTATTTAGTCCTCAAATATGTCTAAGAAA106230.12210138518349173No Hit
GGTAGAAGTAGGCTCCCTGCTGAGCAAGGAGCCAGATATGGGACTCCGGA105780.12158415254362943No Hit
TAATAATCCTGATAGTGGAAGCAATAATATTTAAGCACTTTTGGTATGCC105440.12119335454906681No Hit
ATTCTCTAGTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGA103830.11934281110422616No Hit
GATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATT103380.11882557846436387No Hit
ATATTAACTAGAGAATTGAGTGTAGCTGACTGCATCCTAGTTATTATTAT103290.11872213193639142No Hit
GTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAG103080.1184807567044557No Hit
CTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTGGGTGTAAGGAGTAAG102540.11786007753662094No Hit
TTTGAGGACTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTA101680.11687158849155077No Hit
CATTTTAACTTGGCCTAAGGGATTTTTAAAATTATGTACTGAAGTGATAA101130.11623941526505241No Hit
GGAGAATACACAGAGAATATAGTCAAAGCAGTGGATACAAAGTGTCAGAT101000.11608999250242553No Hit
AATTAAACCAATAAGGAACAAAAGTCACTGTTGGATAATAATAACTAGGA100470.11548080739325439No Hit
GTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTTCCTTATAGGAAA99810.11472219952145636No Hit
ACTATATTCTCTGTGTATTCTCCTTTCCTTAAATAGAACTATTAGCTATG99690.11458427081749308No Hit
ATATGGGACTCCGGATCAAGCCCTGAGCTGAAGGCAGGTGCTTAACCAAC99430.11428542529223931No Hit
TATTTGAGGACTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATAT98120.11277970360730687No Hit
GTCTGTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAGT97600.11218201255679933No Hit
ATCTTTCACTGAGTGTTTTGCCAAATATTCTGTGAGATTTGGCACATCAT96860.11133145221569245No Hit
AACTAAGAGCCTGGAAATCTAGGAATCCCTACTAAACTCTTTTCCTATAA96640.11107858292509311No Hit
ACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATTGGATA96380.11077973739983935No Hit
GTACCTATTTCTTAGACATATTTGAGGACTAAATAAGTGTATGTGGCATA96090.11044640969859476No Hit
CTATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAA95670.10996365923472326No Hit
ATTTATTTGTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGC94980.10917056918693442No Hit
GTTATATATATACAATGAACTAATTATAAAGGAATATACAATGAAAGTTA94850.10902114642430755No Hit
GCATCAGAATCATCTGGGATCACTTGGAGAAGTAAACTACAGATTCTGCA94340.10843494943246362No Hit
ATATTTGGCAAAACACTCAGTGAAAGATAGCCCATAGTACCTACTAGTTC94130.10819357420052789No Hit
GAAATAGGTACTGTTCTTTTTCCATTTTACAGATGAAAAGACTGAGAAAT93260.1071935910967941No Hit
CTATCAGGATTATTACCACCACCAAAAGGTGGCATTGATGACCAGAAGAA92540.10636601887301445No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG91640.10533155359328986No Hit
TAATGAAACTACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAG91590.10527408329997182No Hit
ATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAAGA91550.1052281070653174No Hit
TTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGAAAAAGAACA91490.10515914271333578No Hit
TACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGAAAA90550.10407870119895676No Hit
ATTAGAAACTGTTTCTTTGTGACTGATGGTGCCTTGAGAAAGCCAGTGGT89180.10250401516204266No Hit
GTCCCATATCTGGCTCCTTGCTCAGCAGGGAGCCTACTTCTACCTCTGCC88090.10125116276770957No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT87730.10083737665581972No Hit
CAGATGATGTGCCAAATCTCACAGAATATTTGGCAAAACACTCAGTGAAA87490.10056151924789317No Hit
ATTTAACATGGGAGATGTTTAATTTGGTCCCGGCAAAATTTTGAATTCTG87220.10025117966397579No Hit
ATTCTTCACAGTGCTTTGTACCCAGTAGAGACAGTGAGTAATAGGGTGTT87090.1001017569013489No Hit
AATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTGGGTGTAA87070.10007876878402171No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)