FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973809
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC91860.9433061308737134No Hit
GTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGAT69420.7128707990992074No Hit
AGCGAAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGA68410.7024991553785188No Hit
TCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCA67820.696440472412968No Hit
GTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAA66230.6801128352685177No Hit
AGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAG63660.6537216230287458No Hit
CCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGC53680.5512374603233283No Hit
AAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTA48870.5018437907228214No Hit
GCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCT46460.4770956111516735No Hit
GTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCA40070.4114769939485053No Hit
ACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGA39740.4080882390694684No Hit
CCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCT35200.36146718709726444No Hit
GTCCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGA32110.32973611868446484No Hit
GAGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCC30340.3115600697878126No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTG29330.30118842606712404No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA28900.29677277577019723No Hit
ATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGT28160.28917374967781156No Hit
CATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCC27980.2873253379256096No Hit
GGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCC27970.2872226483838206No Hit
CTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCT27040.27767252099744405No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA26500.2721272857408383No Hit
AGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGAGATGCAGGGC26200.2690465994871684No Hit
CTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCT24040.24686565846074537No Hit
GAATTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTC23500.2413204232041396No Hit
CTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGG23460.24090966503698363No Hit
CTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAG22780.23392677619533195No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT22080.22673850827010225No Hit
GGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAG21270.21842065538519362No Hit
CCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCA21060.2162641750076247No Hit
CGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAAAT20920.21482652142257877No Hit
TCTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCG20700.21256735150322084No Hit
ATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCG20700.21256735150322084No Hit
GATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATG20340.208870527998817No Hit
CTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGA20210.20753556395556008No Hit
TTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTG19990.20527639403620218No Hit
AGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCC19840.20373605090936725No Hit
TCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGC19740.2027091554914773No Hit
GCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACAT19690.2021957077825323No Hit
AGCGAAAGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAG19450.1997311587795964No Hit
GAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGA19300.19819081565276148No Hit
ACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTA19100.19613702481698156No Hit
CACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCC18990.19500743985730262No Hit
GCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGC18720.19223482222899974No Hit
GAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGGGCAGT18380.18874337780817388No Hit
GTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTG18190.18679227651418295No Hit
CCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTG17600.18073359354863222No Hit
GCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACA17530.18001476675610925No Hit
ATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAA17120.17580449554276045No Hit
GATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGAGATGCAGGGCT17110.17570180600097143No Hit
GAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACG16950.1740587733323475No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAC16630.17077270799509967No Hit
CTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACA16490.16933505441005373No Hit
GTAGAAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTTAAAT16430.16871891715931975No Hit
GTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTT16400.16841084853395277No Hit
CACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAA16230.16666512632353983No Hit
TCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTA16180.16615167861459484No Hit
AACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAG16170.16604898907280585No Hit
GCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCC16100.16533016228028288No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTC16030.1646113354877599No Hit
GTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAG15780.16204409694303504No Hit
CAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCG15300.15711499893716324No Hit
CTCCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTT15290.15701230939537425No Hit
GCGAAAGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGC15200.1560881035192733No Hit
CATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTT15100.15506120810138332No Hit
AATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGGATGTGCTCT15090.15495851855959433No Hit
GAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCT14820.15218590093129145No Hit
CTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTC14770.1516724532223465No Hit
GTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGT14740.15136438459697948No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAG14570.14961866238656654No Hit
AAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTTAAATACAT14120.14499763300606178No Hit
GAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGA13910.14284115262849284No Hit
CGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCAC13880.14253308400312587No Hit
CAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACG13740.14109543041807993No Hit
AGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGG13730.14099274087629093No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA13570.139349708207667No Hit
GGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGG13470.13832281278977704No Hit
TCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGAC13450.13811743370619906No Hit
GATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCA13270.13626902195399715No Hit
GCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGA12960.1330856461585383No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC12950.13298295661674928No Hit
GTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCA12890.1323668193660153No Hit
AAGCAGGGGAGTCCAAGGGATGTAACTGCCCCGAGAGGCCTCAAACTGGC12870.13216144028243731No Hit
GCGAAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAA12760.13103185532275838No Hit
GACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTC12670.1301076494466574No Hit
CTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCAC12610.12949151219592345No Hit
CTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGT12560.12897806448697843No Hit
GCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTG12540.12877268540340048No Hit
TCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATC12490.1282592376944555No Hit
GCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGGGCAGTATCCG12480.12815654815266647No Hit
GGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGG12350.12682158410940955No Hit
CAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCA12320.12651351548404255No Hit
TGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTGAC12110.12435703510647365No Hit
GGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGGATG11940.12261131289606073No Hit
GAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGACA11890.12209786518711575No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT11780.12096828022743679No Hit
CCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGAAG11740.12055752206028081No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTAT11610.11922255801702387No Hit
GTCCAAGGGATGTAACTGCCCCGAGAGGCCTCAAACTGGCCCTTTCGTGG11570.1188117998498679No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA11460.11768221489018894No Hit
AAGCAGGGCTGGGACATCGAGCTGGCCGAGGGCGACGACCTGACGTGGCG11430.11737414626482195No Hit
GTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTGCATCTC11350.11655262993051No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG11280.11583380313798701No Hit
GGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGG11100.1139853913857851No Hit
TTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATT11020.11316387505147313No Hit
GAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAA11010.11306118550968414No Hit
GTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGT10940.11234235871716115No Hit
TGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAG10560.10844015612917934No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10550.10833746658739034No Hit
ACCAAAATGAAGGCAATACTAGTAGTTCTGGATGTGCTCTAATGGGTCTC10420.1070025025441334No Hit
AAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCT10360.10638636529339941No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA10350.10628367575161042No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10290.10566753850087647No Hit
GCAGGAGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTC10260.10535946987550947No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG10230.10505140125014248No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT10220.10494871170835349No Hit
AAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGT10210.10484602216656448No Hit
AGCGAAAGCAGGGCTGGGACATCGAGCTGGCCGAGGGCGACGACCTGACG10170.1044352639994085No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10160.10433257445761951No Hit
GGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGAC10110.10381912674867454No Hit
TACTAGTAGTTCTGGATGTGCTCTAATGGGTCTCTACAGTGTAGAATATG10100.10371643720688555No Hit
AGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAA10100.10371643720688555No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT10070.10340836858151856No Hit
GGGCTGGGACATCGAGCTGGCCGAGGGCGACGACCTGACGTGGCGCGACC10040.10310029995615157No Hit
GGATGTAACTGCCCCGAGAGGCCTCAAACTGGCCCTTTCGTGGATGAAGA10010.10279223133078458No Hit
ATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGG10000.10268954178899559No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG10000.10268954178899559No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT9960.1022787836218396No Hit
ATGCAGGGCTGGGGCAGTACATTCAGAAATTCCGTTTGGAGACGAACTCC9950.10217609408005061No Hit
GGGCTGGGGCAGTACATTCAGAAATTCCGTTTGGAGACGAACTCCGGCAA9930.1019707149964726No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT9930.1019707149964726No Hit
CCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCT9840.10104650912037165No Hit
AAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGGATGTGCTC9760.10022499278605969No Hit
TTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGT9760.10022499278605969No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)