FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617648
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG120320.45964927293509283No Hit
GTAATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA88660.33870100181537016No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC82110.3136785389020984No Hit
GTCCATTACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA78110.2983976455199477No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAA63320.24189654223944548No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG62700.23952800376521216No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA62230.23773249879280942No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT61830.23620440945459434No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT56720.21668306815889682No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT56580.21614823689052157No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC54610.20862239689981235No Hit
ATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGA53660.20499318472155156No Hit
GTATGACAATTGGAATGGCTAACTTAATATTACAAATTGGAAACATAATC52550.20075273680800476No Hit
GTATATAGCCCATCCACTAACAGGGCAGAGAGAGGAATTGCCCGCTAATT51810.19792577153230684No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA51760.19773476036503No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG47020.1796269017071814No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG46100.17611229622928676No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC45320.17313252201976737No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA44930.17164263491500767No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA44410.1696561187753281No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT43670.1668291534996302No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT43050.16446061502539683No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA42450.16216848101807424No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT40090.15315275392260533No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA39390.15047859758072896No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT39140.14952354174434454No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA38680.14776623900539723No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA38290.1462763519006375No Hit
CATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGATA37980.14509208266352086No Hit
GGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATG37780.14432803799441332No Hit
CACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGA37260.1423415218547337No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA36350.13886511861029444No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA35220.13454826622983684No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAATACGGCAATG34970.13359321039345243No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT34410.13145388531995136No Hit
GTCCTATTGGTGAAGTTCCCTCTCCATACAACTCAAGATTTGAGTCAGTC34350.13122467191921908No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT34110.13030781831629004No Hit
GCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA33860.12935276247990563No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA33770.12900894237880722No Hit
ACAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCT33270.12709883070603842No Hit
CTAATGAGCTGTCCTATTGGTGAAGTTCCCTCTCCATACAACTCAAGATT33260.12706062847258304No Hit
GACTGGGACAGACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAA32930.1257999547685556No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA32770.12518871903326956No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG32100.12262916939175932No Hit
TAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAA31920.12194152918956255No Hit
GTATGAGGCCTGTCCATTACTTGGTCCATCGGTCATTACAGTAAAGCAAG31780.12140669792118726No Hit
GTATGGAGAGGGAACTTCACCAATAGGACAGCTCATTAGGGTTCGATATG31330.1196875974156953No Hit
GTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACA31120.1188853505131324No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC30800.11766287904256036No Hit
GTCTTTAATGGTTCCATTGGAATGTTTGTCATTTAGCAAGGCCCCTTGAG30470.11640220533853292No Hit
TTCCAAGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCC30170.11525613833487161No Hit
GACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTC29720.11353703782937966No Hit
TCCACTAACAGGGCAGAGAGAGGAATTGCCCGCTAATTTCACGGAAACCA29680.11338422889555816No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT29600.11307861102791514No Hit
AGTTATCCCTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAG29540.11284939762718288No Hit
ATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGTGC29280.1118561395573431No Hit
TTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCTTGTGTT29100.1111684993551463No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT28820.11009883681839575No Hit
CATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG28510.10891456758127906No Hit
TCCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTT28510.10891456758127906No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG28470.10876175864745756No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC28430.10860894971363606No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC28340.10826512961253766No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT27910.10662243357395645No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG27740.10597299560521506No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC27590.1053999621033844No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG27520.10513254646919677No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT27510.1050943442357414No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA26970.10303142362915105No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA26880.10268760352805267No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG26780.1023055811934989No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC26770.10226737896004351No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC26740.10215277225967738No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA26720.10207636779276663No Hit
GCATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACG26650.10180895215857899No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC26510.10127412089020374No Hit
TCCCTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTC26400.10085389632219458No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG26390.1008156940887392No Hit
GGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTGGATTG26270.10035726728727468No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC26230.10020445835345318No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA26180.1000134471861763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)