FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558239
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGACAGCAATTGGAGAAGACAAGACCAGCAGCAGGCTTGGGGACA48750.3128531630898726No Hit
CTGTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCCTGGAGGGGAAC42680.2738989333471951No Hit
TCCCAGAGGAGTCCAGGGCCAAGGGAAAGTGAGACTGGAGCATCTGAGAG38730.24854980526093878No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT35960.2307733280966527No Hit
CTCTAGGCCTCAGCATAGTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTG35370.22698700263566757No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA34390.22069785186996346No Hit
GTCCAGGGCCAAGGGAAAGTGAGACTGGAGCATCTGAGAGAAAGTAGGAG32430.20811955033855525No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT31280.2007394244400249No Hit
GCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGAGTGGTG28370.1820644971663525No Hit
GTCCTGCACCACTCAGCCACCTCCCTGCATCACAGGTGTGACCACAGGGA28080.18020342193976663No Hit
GGCCTAGAGAGGCTGGGCTCATTGCTGGGAACCCAACCAAAGGGGCTGGT27220.1746843712678222No Hit
GGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCT26590.17064134577558385No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT25230.16191354471297406No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG25130.161271794634841No Hit
CACCCAGACTATGCTGAGGCCTAGAGAGGCTGGGCTCATTGCTGGGAACC24440.15684371909572278No Hit
GTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTGTCTTCTCCAATTGCTGT24350.15626614402540304No Hit
CCTCTACAGCGTCCCAAGACTGAATATCCAGGTAGAGCCTCCTACTTTCT23660.15183806848628484No Hit
AACCAAGGGTGTCCAGGCAAGACAGGGAGGTGGCATGGGAAGGGTGGAAT23370.14997699325969893No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC22970.14740999294716664No Hit
TCCTAGGCCTGGAGGGGAACATGACCAGCCCCTTTGGTTGGGTTCCCAGC22710.14574144274402065No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA22480.1442654175643146No Hit
GTCTTGGGACGCTGTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCC22160.14221181731428875No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT21840.14015821706426293No Hit
TGCTTAGCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGA21110.1354734414938915No Hit
GTGTATGAGGGGGTACTCCAATGCACCGTCAACCCTGTCCTTGGTATTTA20840.13374071628293221No Hit
ACCCTGGACTTGCCAGACTACTGCTAAGTTTTTTGTTTGTTTGTTTCAAG20610.13226469110322614No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA19440.12475621518906921No Hit
GTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCCTGGAGGGGAACAT19240.12347271503280305No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA18970.12173998982184377No Hit
GTTTGGGTGTGCCAGACTCAAACCAAGGGTGTCCAGGCAAGACAGGGAGG18940.12154746479840384No Hit
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC18030.11570753908739287No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC17880.11474491397019328No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA17820.11435986392331343No Hit
GTCCAGGGTTGGTCCCTTACTCGACCCTTCGAACATTTAAGTACAGGAAG17750.11391063886862028No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG17630.1131405387748606No Hit
GCTCCAGACCATCCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAAT17150.11006013839982186No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC17090.109675088352942No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC17080.1096109133451287No Hit
GATCAGGAAGGGATGACATGCTTAGCCTTATCCCTGTGGTCACACCTGTG16780.10768566311072948No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTTC16760.10755731309510287No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT16090.10325758757161128No Hit
CATTTATGCAAGCCTTACAACTACTGCTTGAAGTAGAACAAGAGATAAGA15920.10216661243878505No Hit
ACTCAGAGCAGCCTGAAAGGCTTCCCAGAGGAGTCCAGGGCCAAGGGAAA15920.10216661243878505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)