FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525513
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGAGGAGCCAGCCTGTGGGGCAGGGAGAGGAGGGCTGCAGCACC215700.6118258534289903No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG144690.4104083575922142No Hit
CTACTGGGCTGTGGCTCCTTCTCCCCCTGGAGAATGGAGGGCCGGGTGAT120850.3427869929851344No Hit
TCCCTAGGCTGTGTAATTTAAGCCACAGCCCCACCTTCCCCATATCATGT113490.32191059854268017No Hit
CCCTAGGCCCAGGGGTTCCTGGTCTGTAAATGGGGAGAGACGGCCTGCAC113080.3207476472218369No Hit
GTGCAGTGCTGGGCAGGGGGTGAACACTCCCTGTTCCTGGGTCTTCTCCC90300.2561329372491323No Hit
GTACCAGGGATGTGAGCAGAAATGACAACGGCTTTCTAAGTGCAAGGCAA79670.22598129690629423No Hit
GGCCTAGGGAGCGTGTGTGTCTAGAGTGTGAATGGATCACAGATGAATGA77050.21854975431944232No Hit
GCACTGCACTGAGCGTTTTGTGGATTGTCTCATTCAGTGTTCACCAGTCC76790.2178122729940295No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG66290.18802937331389788No Hit
CTCTAGACACACACGCTCCCTAGGCCCAGGGGTTCCTGGTCTGTAAATGG64070.18173241738152718No Hit
ATACAGGTCAGTGTGAATGACATCCGAATTATATTAGTGGAAAAACTTGT61840.17540709678279445No Hit
CCCTGGTACCTAGCAAAATGCCATGCGCACTAAATGGCCAGGATGACTCA59520.16882649418680346No Hit
CTGTGAGACCTGCCTGGCCCTGCTTTTGGTCTTATTAACCCACTCAGTTC56990.1616502335972098No Hit
GTCTCACAGTGAGCAGACTGGCCTGGTGGAACTTGGAACCAACTAGAGAT56200.15940942495460944No Hit
GTGCTGGGCAGGGGGTGAACACTCCCTGTTCCTGGGTCTTCTCCCTCCAA53770.15251681102863612No Hit
GGTCAGGAGTGGGGACTTTATTCAACATGTGATGGGAAGCCTGGGGAAGG52580.14914141573155454No Hit
TGTCTAGAGTGTGAATGGATCACAGATGAATGAACACAGAGCCCATGGGT46500.1318956985834402No Hit
GCCCAGCACTGCACTGAGCGTTTTGTGGATTGTCTCATTCAGTGTTCACC46390.13158368725345787No Hit
GTGTGTATGTGTGCATGTGGGTGTGTAAACTGCTTTCCCAATACCAGTAG46350.13147022858800975No Hit
TCCCAGGACCCTGGGATCATGACCTGAGCCAAAGATAGATGCTTAATGAC46040.1305909239307868No Hit
GTCTAAGGGTTGACTTTTCTACAATAAGCTGCCCGATTTAATCAAGAGGA45370.1286904912845308No Hit
CTTTAAGCCCAGGCGTGCCGTGGTCTGATCTATGGATTTAAAAATCCTCC44040.12491799065838079No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC43240.12264881734941836No Hit
CCCATGGGCTCTGTGTTCATTCATCTGTGATCCATTCACACTCTAGACAC43230.12262045268305634No Hit
GCCCATGGGTCCTCATTTCCACTTGGTCCCAACTGGCTGAGGGGTGGCTG43000.12196806535672966No Hit
GGGTAAAGGCTTGGGGCCCAAGGGTAGAGGAAAGATGGCTCTGAAACCAC41370.11734462473971873No Hit
GTCCTTTAGTTCTCCCAACAATGCTGCAGTGCAGGCCGTCTCTCCCCATT40540.11499035743167023No Hit
AAACTATACAGGTCAGTGTGAATGACATCCGAATTATATTAGTGGAAAAA39740.11272118412270782No Hit
CTCCTGACCATGGCCAGCGAGATCAAGAGGGACCTGCTCCCTCCTTCCCC39480.11198370279729504No Hit
CCTCTAAGGCAGCTTCCTACTATGGGGGTTGTTTCCCATTTAGGGACCAG39270.1113880448036924No Hit
GCTCTGGACACTACTGGGCTGTGGCTCCTTCTCCCCCTGGAGAATGGAGG38940.1104520108137454No Hit
AGCAGGGACAGTGGCAGAGGGAGAAGCAGGCTCCCCACTGAGCAGGGAGC38590.10945924749107434No Hit
ATATGGGGAAGGTGGGGCTGTGGCTTAAATTACACAGCCTAGGGAGGAGC37670.10684969818576759No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA37180.1054598295340281No Hit
GCCCTGAGGACGTCAGGAGTGAAGCACATGATATGGGGAAGGTGGGGCTG36350.1031055622259796No Hit
TCCCCATATCATGTGCTTCACTCCTGACGTCCTCAGGGCTGAGGGCTGTT35680.10120512957972358No Hit
GTGTGAATGACATCCGAATTATATTAGTGGAAAAACTTGTTCCTACACTC35350.10026909558977658No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)