FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186678
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCT19370.16322877815211878No Hit
GTCTATAACTGTGATTATTTAGCTTCTCTCTTATTCAGCCTTCTCAGGGT17000.14325705878089928No Hit
GTTATAGACAACACAGAAGATTCTAGGTGGCACGTTCTTAAAAATCGAGG15490.13053246120683118No Hit
CCGTACACCGAGCACAAGTAGTGGCCTCTGAGTCCAACATTGTTGATATC14740.12421229684885032No Hit
GTGTACGGAGCGCGGCTCATGGTTCCAGCTGGCCGGGGGCTCATCGTGGT14270.12025166051784898No Hit
GTCTAGTCTCCAGGTAAATATCATTGCTCCATGCATGCACTTTAGCCATA14030.11822920792329511No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG13940.1174707882003374No Hit
ATCTTACTTTCTGGTACAAATGGAAGATTTGATCCATAAAGAAATTCAAA13770.11603821761252842No Hit
GTCTTAAAGCGTTACCATTAAAAAGGAATTATCCTACTTAGGAAAAAAGG12550.10575741692354623No Hit
GTCCGTAGCCAGCCTGTCGCACGCAGCTTTGCCCACGCCATAGGGGACAT12190.10272373803171543No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTTC12040.10145970516011926No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)