FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5535278
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGC381730.6896311260247453No Hit
CCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAGTCAAGAATCTG372800.6734982416420638No Hit
GTGATAATGCTGGTGGGGGCACACAGGCAGAGTCCCCACGTGCTGTAGAG291830.5272183258004386No Hit
GTGCCACACAGTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTG286450.5174988501029216No Hit
CTTTCAAACCATCAGCTCTCCTGAGAACTCCCTCACTATCAGGGGAGCAG254150.45914586403790375No Hit
CTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATCCCCTCC251920.45511715942722303No Hit
CTCCTGAGAACTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCC241490.4362743840508101No Hit
GTTCTGTACTGTTCTGAATTTGTAACATCTGGTCTCTGCTCAGGCGAACA231910.4189672135708451No Hit
AATTAACACTGGCCATTAAGAAGCTGCGGTGAGTAAAACGTCAAGCTCAG214580.38765893962326736No Hit
CCTGAGAACTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCC204790.3699723844041799No Hit
GGATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGACCTCA195110.35248455452463273No Hit
CCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACAC190580.34430068372356365No Hit
GTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGG188210.3400190559534679No Hit
GTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGC186390.33673105488107374No Hit
GTCCCTACACATAGTGATCTCTTCTGTGCAGTATTGTTAGTGAAGCTCGT173500.3134440582749412No Hit
GGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAA171830.31042704630192014No Hit
GTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACATGGG164370.29694985509309557No Hit
GAACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACC163950.2961910856148508No Hit
ATCTTGCAGCTCCCCTGGGGCTGGGGCTGAGTACAGTTCTGCTGGCCCTG159810.2887117864721519No Hit
GTGTAGGGACATGGCATCAAAGTTCTGGCAAGGAGGGGCGCCTGGGTGGC148100.26755657078108813No Hit
CCCCTGGGGCTGGGGCTGAGTACAGTTCTGCTGGCCCTGCTCTACAGCAC140190.2532664122741441No Hit
ACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCT138940.2510081697793679No Hit
TTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGTGAG134630.24322174965737947No Hit
GTGTTAATTTGTGTTATTTCTTAAGCAATTTACTCGATAACGTTTTGTTA134430.24286043085821524No Hit
GCTCTAGGGACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTA132900.24009634204460914No Hit
GTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCAC128880.23283383418140877No Hit
CCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTGT128800.2326893066617431No Hit
GCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGG125680.2270527333947816No Hit
TTCCAGGAGAGAGCAGGAGGCCTGACAGTTTGCGGGAGCCCTGGAGGAAA124970.22577005165774872No Hit
CCCTAGAGCCCTGCGTGTCTGCTCCGGCTGGCAGTAGAAGGAGCCTCTCC122510.22132583042802906No Hit
GGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGC120900.21841721409475728No Hit
CTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGA120450.21760424679663787No Hit
CACAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACT120120.21700807077801693No Hit
TCCCTACACATAGTGATCTCTTCTGTGCAGTATTGTTAGTGAAGCTCGTT119350.21561699340123477No Hit
CTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAACTTCATGAGGCC118610.2142801138443272No Hit
CTATAAAACACGTCAAATGTCAAGTTTATAACAAAACGTTATCGAGTAAA118360.21382846534537198No Hit
CTCCAGGATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGA117020.21140762939097185No Hit
GTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAG116370.2102333432936882No Hit
CCTCCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAGTCAAGAAT112110.20253725287149082No Hit
CATTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAG111710.20181461527316244No Hit
ATGCTGGTGGGGGCACACAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGC109960.19865307578047572No Hit
GTGCCACACTTTCAAACCATCAGCTCTCCTGAGAACTCCCTCACTATCAG107700.19457017334992027No Hit
GATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAA106880.19308876627334706No Hit
ACCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTGGGGCTGA106290.1920228758158127No Hit
ACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCC105840.19120990851769323No Hit
GCTAAATGCTGTATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGT104630.18902392978274984No Hit
CCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTG103840.18759672052605125No Hit
GCTCTACAGCACGTGGGGACTCTGCCTGTGTGCCCCCACCAGCATTATCA100230.1810749162011375No Hit
GGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGG100020.18069553146201509No Hit
GTTTATAACAAAACGTTATCGAGTAAATTGCTTAAGAAATAACACAAATT99870.18042454236264194No Hit
CCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAACTTCATGAGGCCCG99580.17990063010385388No Hit
CCCCAGATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGAT99260.17932252002519114No Hit
GAATTAAAGAGTCAAGAATCTGAAGGCTTTTCAGACAGAACAGGTCCCTA99200.17921412438544188No Hit
GTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGCTG97910.1768836181308328No Hit
GGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACC97410.1759803211329223No Hit
GCCACACAGTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTGAA95530.17258392442077886No Hit
GAGCCCAACCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTG94710.17110251734420567No Hit
GGTTGGGCTCTGTGTCCCCACCGAAATCTCATCTGGAATTGTAATCCTCC93930.1696933740274653No Hit
TCCCAGGACCCTGGGATCATGACCCAAGCTGAAGGCAGAGGCTTTAAGCC92050.16629697731532184No Hit
GCTCTATGGAGGTCACTCACTGTGTGGCACCGGGCCTCATGAAGTTGTAG91450.16521302091782925No Hit
GTATTGTTAGTGAAGCTCGTTGGCCTGGCAGGATCAGGTAGTCTTGTGTC91310.1649600977584143No Hit
ATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAAT91100.1645807130192919No Hit
TCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATT90740.16393033918079633No Hit
GCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTG90570.16362321820150677No Hit
TCCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACA87980.15894413975233043No Hit
CAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCA87960.158908007872414No Hit
GTTCTGAATTTGTAACATCTGGTCTCTGCTCAGGCGAACAATTTCACAAA87680.1584021615535841No Hit
CTTCTGGACCTCAAATCCCAGGTACTCAGAAGGGGAAGGCAGGAGAGGCT86950.1570833479366348No Hit
GGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAAT86720.15666783131759598No Hit
GTATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACA86640.15652330379793028No Hit
ATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATCCCCTCCCA86380.15605358935901684No Hit
GGCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATC86260.1558367980795183No Hit
CTTCTGTGCAGTATTGTTAGTGAAGCTCGTTGGCCTGGCAGGATCAGGTA86220.15576453431968548No Hit
CTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCA85560.15457218228244363No Hit
GGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGT85450.15437345694290333No Hit
ATCTACAACTTCATGAGGCCCGGTGCCACACAGTGAGTGACCTCCATAGA85430.15433732506298692No Hit
GCCTTAGCTAAATGCTGTATGTCATAGATCATCTGGTTCTTGTGGGGTGT85230.1539760062638227No Hit
GCCTCAGACTCCCCACGCTGAGCCCAGGCCCCTACCCTCTGAAGGTGTTT84500.15265719264687339No Hit
AAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGG83780.15135644496988226No Hit
AGCAGGAAGTGCCACACTTTCAAACCATCAGCTCTCCTGAGAACTCCCTC83690.15119385151025838No Hit
GTGTGGCACCGGGCCTCATGAAGTTGTAGATCCAGCTCCCACCCAGAGAA82940.14983890601339264No Hit
CTGCAGGGCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGC82790.1495679169140195No Hit
CCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGAGAT82140.14839363081673584No Hit
CTTGTGGGGTGTGTGTGTCAGAATCACATGGGAAACACCTTCAGAGGGTA81550.14732774035920146No Hit
CCCCTACCCTCTGAAGGTGTTTCCCATGTGATTCTGACACACACACCCCA81060.1464425093012492No Hit
GTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCA80090.14469011312530283No Hit
TTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAGGG77320.1396858477568787No Hit
GCTCATTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAG77050.13919806737800702No Hit
GTACTCAGAAGGGGAAGGCAGGAGAGGCTCCTTCTACTGCCAGCCGGAGC76520.1382405725602219No Hit
GCCCAGGCCCCTACCCTCTGAAGGTGTTTCCCATGTGATTCTGACACACA76010.1373192096223532No Hit
GAACAGGTCCCTAGAGCCCTGCGTGTCTGCTCCGGCTGGCAGTAGAAGGA74510.13460931862862172No Hit
CCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATC74260.13415767012966648No Hit
CTCACAGGTTGGGCTCTGTGTCCCCACCGAAATCTCATCTGGAATTGTAA73580.1329291862125082No Hit
CCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGA72850.13161037259555888No Hit
GGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTG72450.1308877349972305No Hit
GCCCAGCTCCTCCCACTCTGCACCTGCTGCCTCAGACTCCCCACGCTGAG71930.12994830611940358No Hit
GGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGGGGCAC71740.12960505326019758No Hit
GACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGAAATCTCATCT71100.12844883310287217No Hit
AGCAGGTGCAGTGCCCAGCTCCTCCCACTCTGCACCTGCTGCCTCAGACT70630.1275997339248363No Hit
GATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGACCTCAA70620.1275816679848781No Hit
AGCGAAAGCAGGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCA70530.1274190745252542No Hit
AAAGCAGGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGC69820.1261363927882213No Hit
GACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATTCAGCC69780.12606412902838846No Hit
GTACAGTTCTGCTGGCCCTGCTCTACAGCACGTGGGGACTCTGCCTGTGT69630.1257931399290153No Hit
GAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAAC69400.12537762330997648No Hit
TCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAGGGGAAGGCA68870.12442012849219136No Hit
AGCGAAAGCAGGGCCTGCAGGGCCATGAGTGGGAGCCCTCCTCCAGTGTT68710.12413107345286001No Hit
GTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGGGGCACACAGG68220.12324584239490771No Hit
CTAGGGACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATT67810.1225051388566211No Hit
GCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCT66300.11977718192293142No Hit
TTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGTGAGGGTCTC66070.11936166530389258No Hit
GTCTTGTGTCTACCTGTGTTCCTTCTTCTTCTTCTTTTTTTAACCTACAT65450.1182415770264836No Hit
CTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATCCC65250.11788025822731939No Hit
CAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTG63200.11417674053588636No Hit
CAGTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAG62710.11329150947793408No Hit
GGCCTGCAGGGCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGG62640.11316504789822661No Hit
CTGTGTCCCCACCGAAATCTCATCTGGAATTGTAATCCTCCTGTGTCAAG62330.1126050037595221No Hit
AAAGCAGGGAGCCTTAGCTAAATGCTGTATGTCATAGATCATCTGGTTCT61540.11117779450282353No Hit
ATCAGGAGGCGGTGATAATGCTGGTGGGGGCACACAGGCAGAGTCCCCAC61300.1107442119438265No Hit
GAGTACAGTTCTGCTGGCCCTGCTCTACAGCACGTGGGGACTCTGCCTGT59160.10687810079276958No Hit
CTTTAATTCAGCCAACATTTCGTGGGTCGCTCTATGGAGGTCACTCACTG59060.10669744139318747No Hit
GTGCCCAGCTCCTCCCACTCTGCACCTGCTGCCTCAGACTCCCCACGCTG58770.1061735291343994No Hit
GTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAAT58380.1054689574760292No Hit
GGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCG58350.10541475965615457No Hit
GAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCA57710.10425853949882914No Hit
CCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTGGGGCTGAG57420.10373462724004105No Hit
ATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACATG57060.1030842534015455No Hit
GCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGAAA56880.10275906648229774No Hit
GTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATTCAGCCAACAT56630.10230741798334247No Hit
CTCTGAAGGTGTTTCCCATGTGATTCTGACACACACACCCCACAAGAACC56380.10185576948438724No Hit
GATAATGCTGGTGGGGGCACACAGGCAGAGTCCCCACGTGCTGTAGAGCA56100.10134992316555735No Hit
CCATCAGCTCTCCTGAGAACTCCCTCACTATCAGGGGAGCAGCAGGGGGG55760.1007356812069782No Hit
CCTCCAGAAGGGGGGCTGTGGGGCCTCCAGGATAAGGAGGGGGCGGCTCA55670.10057308774735432No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)