FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339303
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA108110.8072109149311246No Hit
CAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTT94560.7060388873914267No Hit
GGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCA91880.6860284789924311No Hit
ATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTT76390.5703713050743558No Hit
TAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAG73750.5506595594872855No Hit
ATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCT69650.5200466212649415No Hit
AGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAA57470.42910379503368545No Hit
CCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACA57300.4278344780830029No Hit
TTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTT57030.4258185041025071No Hit
ATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCA56590.4225332131713287No Hit
CTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTG53240.3975202026725842No Hit
GCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGAT50190.37474716326327945No Hit
ATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAA49590.37026722108439986No Hit
CTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTC42560.3177772318885271No Hit
GTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTC42290.3157612579080313No Hit
ATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGAC42140.3146412723633114No Hit
TATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAAC41510.30993733307548776No Hit
GTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAG41380.3089666789367305No Hit
GATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTA40170.29993212887598997No Hit
GTTTAGCACCAGGTTCCCCACGAACGTGCGTTGCGTGACGGGCGAGGGGG39680.296273509429905No Hit
GCTTTATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGT39120.29209223006295065No Hit
CAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAA38290.2858949767155005No Hit
TATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCA36440.27208182166395506No Hit
CTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAG35860.2677512108910381No Hit
AGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCATAAAA34390.25677535255278305No Hit
AGCAGGGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCG33570.25065276490831423No Hit
TCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCT33140.2474421396801172No Hit
GTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTT32650.24378352023403219No Hit
ATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAAT31040.23176234205403856No Hit
GTTTTATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGC30800.22997036518248673No Hit
GCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTG30700.22922370815267346No Hit
GTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTC30350.22661040854832698No Hit
AGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATC30160.2251917601916818No Hit
AGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGA29760.2222051320724287No Hit
GCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGAC29450.2198904952800076No Hit
GCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAA28930.2160078787249786No Hit
CTCTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGT28830.21526122169516532No Hit
GTACTAAGGCACCATAAAAATAAAACAGATAAAATTGGTTTTAAAAATAG27760.20727199147616335No Hit
ATCCCAGGAAGGACCCTGGGATCATGACCTGAGCCAAAGGCAGACACTAA27660.20652533444635007No Hit
GCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGC26710.19943209266312403No Hit
GTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGCTCTTT25660.1915921938500847No Hit
GGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTT25460.19009887979045817No Hit
ATCCCAGGACGCAGGGGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCA24260.18113899543269896No Hit
GGTCATGATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCC23930.17867502723431516No Hit
AGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTT23800.17770437309555792No Hit
TAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTC23760.1774057102836326No Hit
CCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGG23730.17718171317468862No Hit
CACCCAGGCATCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTT23530.1756883991150621No Hit
CTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAA23480.17531507060015544No Hit
CTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCT23180.17307509951071565No Hit
TCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTG22990.17165645115407044No Hit
GGATAAGTAGGCTTCCCACTGAGCGGGGAGCTCCATGCAGTGCTCAATCC22890.17090979412425716No Hit
GCACTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCA22430.16747517178711613No Hit
GATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAA22300.16650451764835889No Hit
GAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTG22280.16635518624239623No Hit
CACTTAGCCAGTGGACACACCCAGGCATCCCTGTTATTAGCTATTTTTAA22260.16620585483643358No Hit
GACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTT21850.1631445610141992No Hit
CCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAAT21620.16142724984562867No Hit
GAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTC21540.16082992422177805No Hit
GTTAGTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGA21500.16053126140985274No Hit
ATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAG21290.15896328164724488No Hit
GGTAAGACTTCTGTGGTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAG21170.15806729321146895No Hit
CAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAG21140.15784329610252495No Hit
GCTAATAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTC20790.15522999649817854No Hit
ATCATGACCTGAGCCAAAGGCAGACACTAACCGACCAAGCCACCTAGGCA20660.15425934235942126No Hit
TCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTAC20250.15119804853718688No Hit
GAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGC20140.1503767258043923No Hit
CTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGT19950.14895807744774708No Hit
ACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTC19900.14858474893284043No Hit
CTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTC19830.14806208901197115No Hit
GCTCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAACTGAGCTGA19600.14634477784340064No Hit
GCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCAGGTCATGATC18880.1409688472287451No Hit
ATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCA18860.14081951582278243No Hit
CAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATT18480.13798221910949202No Hit
CCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGT18360.13708623067371611No Hit
AATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGG17620.1315609686530979No Hit
GGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAA17030.12715569217719963No Hit
GATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAA16970.12670769795931167No Hit
GGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGACCCCTGCGTCCTGGGATT16950.126558366553349No Hit
GGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGG16930.12640903514738638No Hit
GAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCA16860.12588637522651708No Hit
GGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCAGTGGACACACCCAGG16710.12476638968179717No Hit
ATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTAT16570.1237210698400586No Hit
CCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTT16520.12334774132515196No Hit
GTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATT16380.12230242148341339No Hit
CTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTAATCCTACTACCACTA16330.12192909296850675No Hit
GATTAATATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAG15950.11909179625521633No Hit
TTATTTGACAGAGAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAG15840.11827047352242173No Hit
TTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATC15660.11692649086875785No Hit
CTTCTGTTTCCCCTGCTTGTGCGCTCTTTCTCTCTCTCTGTCAAATAAAA15640.11677715946279521No Hit
GTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGATTGA15460.11543317680913132No Hit
GAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTCTC15420.115134513997206No Hit
AGCGAAAGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT15100.11274521150180355No Hit
ACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGG14960.11169989166006497No Hit
AACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCA14950.11162522595708364No Hit
GCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCT14920.11140122884813967No Hit
CTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGA14860.11095323463025171No Hit
GTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATT14820.1106545718183264No Hit
AACTAGGATGCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGT14650.10938525486764385No Hit
GTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACT14540.10856393213484926No Hit
GGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGA14300.10677195526329741No Hit
TCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTTTTATTTTTAT14110.10535330690665219No Hit
CCCCACGAACGTGCGTTGCGTGACGGGCGAGGGGGCGGCCCCCTTTCCGG14100.10527864120367086No Hit
ACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAG14020.10468131557982024No Hit
AGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGAC14010.10460664987683892No Hit
ATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGACGACAAACTG13950.10415865565895097No Hit
GGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCGGGGAG13920.10393465855000698No Hit
TCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAACTGAGCTGAAG13850.10341199862913769No Hit
GGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTT13760.10274000730230576No Hit
CCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTC13720.10244134449038043No Hit
GATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACT13650.10191868456951117No Hit
GTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATCT13610.10162002175758585No Hit
ATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGC13480.10064936761882859No Hit
TCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGA13480.10064936761882859No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)