FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3996957
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA747731.870748171671599No Hit
TCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGG415991.040766763315192No Hit
GGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTG284150.710915829217077No Hit
ACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGC263080.6582007262024585No Hit
GTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGA260910.6527715959916507No Hit
GCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGGGCAGATC236690.5921754975097305No Hit
GTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCCT211630.52947780023653No Hit
CTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAACGTTTG197330.4937005827183029No Hit
GTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCT193660.4845185975230657No Hit
GTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCA193510.4841433120246227No Hit
GGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATC184910.4626269434472275No Hit
AGCGAAAGCAGGGCAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCC178290.44606434344927903No Hit
ATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGG171630.4294016673184125No Hit
TTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGAT157420.3938496210992512No Hit
GCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCAGT156600.3917980603744298No Hit
CCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTA154680.3869944059943602No Hit
GTCGAGAGGAGGGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCA145500.36402693348965226No Hit
CCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTC131680.32945062956644267No Hit
CCATAAAGGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGGCTGTCTGCCC130580.3266985359111944No Hit
GGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTT127690.31946803530786044No Hit
GGGCAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACT123030.30780916582289974No Hit
AGCGAAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGA120220.30077881748540203No Hit
GTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCA119060.2978766096307766No Hit
TCCATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTG113070.2828902087262885No Hit
GCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATA111640.2793124869744658No Hit
CTGTTACACCATTTTGCTGACCGGGCAGATCTGCGAGGGCGTCCAGAAAA111090.2779364401468417No Hit
GCCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGC110480.2764102791198404No Hit
AAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTT109190.2731828238332311No Hit
GCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTT108580.27165666280622985No Hit
CATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGAC108050.2703306540450648No Hit
GCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTAATGCTAGGGC107930.2700304256463104No Hit
GCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCACA107580.2691547594832769No Hit
GGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCC106320.2660023612963562No Hit
ACTGTAAGCTCCATAAAGGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGG104980.26264981084359923No Hit
TATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGG104120.2604981739858597No Hit
TATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCATTT102580.2566452428685122No Hit
GTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATAGCC100490.2514162649235406No Hit
CTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAG100100.2504405226275889No Hit
GGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTG98720.24698789604191387No Hit
ACCCAGCAGGGAGCCCCATGCAGGGCTCAATCCCAGGACCCCAGGATCAT98680.24688781990899575No Hit
TGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTGG96830.24225929876153285No Hit
GCTCAGATATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGG96200.2406830996680725No Hit
CCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAG95780.23963230027243226No Hit
ACATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCTCAGTCGTTGGGCGTCT95610.23920697670753027No Hit
GAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGA94210.23570431205539613No Hit
CCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGG91380.22862392565143932No Hit
CTTTAAAAAGCCTCAATAAGCATCGAAAGTATTCCCAGGCAACTCTCTCA90570.22659738395984746No Hit
TCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAGTGC90400.22617206039494547No Hit
AGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGC89370.22359509997230392No Hit
GCCCCATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTA89330.22349502383938583No Hit
CATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCTCAGTCGTTGGGCGTCTG88930.22249426251020463No Hit
CTTCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAAC88090.22039266371892416No Hit
AGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCAT87750.21954201658912018No Hit
ACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTC87640.21926680722359534No Hit
GAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCC87000.21766558909690545No Hit
ATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCG85900.21491349544165722No Hit
GCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCATTTCTGTT84430.21123569755691643No Hit
GATATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCAT83990.21013486009481713No Hit
GGGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCA83720.20945934619761983No Hit
GCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCC82280.20585660541256762No Hit
GTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAGAT81920.20495592021630457No Hit
TCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCCAACGAC79920.1999521135703987No Hit
TATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCTCCCTCACCCTCA79440.19875119997538127No Hit
GTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGGCTTTTTAA79200.19815074317787257No Hit
GTCACTGCCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACT78330.1959740872869035No Hit
GGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTT78040.19524853532324715No Hit
GAGTTTCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCC76760.19204609906986742No Hit
AAGCAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGC76580.19159575647173585No Hit
GCCTTAGACCTAGGTCATGATCCTGGGGTCCTGGGATTGAGCCCTGCATG74260.18579134076248507No Hit
CCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCT74140.1854911123637307No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA73140.18298920904077776No Hit
GATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGC72430.18121285768148118No Hit
CCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCCTCTTTAATGTGGCTG72010.18016205828584098No Hit
AGCGAAAGCAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGC71900.17988684892031614No Hit
CAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTC71610.17916129695665978No Hit
TTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGA70690.17685954589954309No Hit
GCCCAGGGCCTTGGCACTTTAAAAAGCCTCAATAAGCATCGAAAGTATTC69980.17508319454024648No Hit
GTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACA69790.17460783290888543No Hit
GAATACTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGC68890.17235611991822777No Hit
TCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGT68870.17230608185176874No Hit
AATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGC68130.17045467339278356No Hit
GGGAAAGACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAGTCGAGCGA67690.16935383593068426No Hit
AGCGAAAGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCA67620.16917870269807755No Hit
GCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCATA67470.16880341719963463No Hit
ATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCA67110.16790273200337158No Hit
CTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACA66280.16582615224532063No Hit
GAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGAT66200.16562599997948438No Hit
TTAAAAAGCCTCAATAAGCATCGAAAGTATTCCCAGGCAACTCTCTCAAC65410.16364949635435155No Hit
CTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGT65160.16302402052361334No Hit
ATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCG65000.16262371599194086No Hit
GGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGC64390.16109755496493958No Hit
CCCATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAG64380.16107253593171006No Hit
CGTTAACGTAATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGG64250.16074728849972617No Hit
CTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGGC64220.16067223140003756No Hit
GGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCC63430.15869572777490476No Hit
GCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGATG63270.15829542324323229No Hit
GGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCT63090.15784508064510078No Hit
GATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGC62970.1575448522463464No Hit
ACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCATAAAGGTAGGA62960.15751983321311688No Hit
GGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCAC62790.1570945096482149No Hit
GCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAA61610.1541422637271304No Hit
TTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGA61510.15389207339483513No Hit
GGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGGCTGTCTGCCCAGGGCCT61310.15339169273024453No Hit
GCTCAGGGCTGATATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCT60960.152516026567211No Hit
CTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCA60680.15181549363678418No Hit
GCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAG60320.15091480844052113No Hit
GTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTT60270.1507897132743735No Hit
TCCCAGGCGCCTCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAG59860.1497639329119628No Hit
GCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCC59050.14773739122037088No Hit
TCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAACGTTT58310.14588598276138573No Hit
GAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGCCCCATGCAGGGCTCAAT57420.14365928880395762No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT56710.14188293744466104No Hit
GCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATAGCCCT56700.1418579184114315No Hit
AAAGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGA55640.13920590088910137No Hit
GACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGG55480.13880559635742892No Hit
ATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTG54070.13527791267206526No Hit
GGCTATAGCCCTCCTCTCGACTGGCTCCCTCACCCTCACAGAGGAAACTC53780.13455236070840892No Hit
TTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGG52940.13245076191712846No Hit
CAGCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGA51260.12824756433456752No Hit
GCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGG51120.1278972978693541No Hit
TCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAAC50730.12692155557340248No Hit
CAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGT50690.12682147944048436No Hit
GGGCTGATATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCTCCCTC50590.12657128910818904No Hit
CCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGA50180.12554550874577836No Hit
CCATTTTGCTGACCGGGCAGATCTGCGAGGGCGTCCAGAAAACAGTCATC48120.12039158790049531No Hit
CTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAA48090.12031653080080672No Hit
TCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGC48040.12019143563465906No Hit
CTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGC47310.11836504620890342No Hit
GGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTTTATGG46320.11588816191918001No Hit
GGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGA46060.11523766705521227No Hit
GAAATGCCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGAT45850.11471226735739215No Hit
GAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGCCCCATGCA44990.11256063049965262No Hit
TCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACG44670.11176002143630766No Hit
CCTCTCGACTGGCTCCCTCACCCTCACAGAGGAAACTCAAGCCACGGAGG44560.11148481207078284No Hit
CCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCT44080.11028389847576545No Hit
TTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTAATG43880.10978351781117486No Hit
CTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGA43440.10868268034907556No Hit
GCCTGGGAATACTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCC43210.10810724258479638No Hit
GTCTTTCCCTTCCTGGCTGTCTGCCCAGGGCCTTGGCACTTTAAAAAGCC42740.1069313480230085No Hit
CCTCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTA42530.10640594832518838No Hit
CTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCACAG42160.10548024409569581No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT41890.10480473019849851No Hit
ATCCATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCT41740.10442944470005555No Hit
GGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAGATATCA41420.10362883563671062No Hit
GAAAGCAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGG40810.10210267460970934No Hit
ATTTTGCTGACCGGGCAGATCTGCGAGGGCGTCCAGAAAACAGTCATCAG40450.10120198941344627No Hit
AAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTC40140.10042639938333087No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)