FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986143
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAG147811.4988698393640678No Hit
CTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCT130731.3256698065087924No Hit
GAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCC123061.2478920399982558No Hit
GACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGA91830.9312036895257584No Hit
CTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATG72970.7399535361504366No Hit
CTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCT68420.6938141831357115No Hit
ATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTC63120.6400694422614164No Hit
TCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCT57640.5844994083008246No Hit
CCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTC51500.522236633023811No Hit
GGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGA51120.5183832365082954No Hit
AAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAG50950.5166593485934596No Hit
CTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCT48480.4916122712426088No Hit
AAAGCAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCT48460.49140946089968696No Hit
CTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCC47980.48654201266956215No Hit
GAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAA43210.43817174588269653No Hit
AGCGAAAGCAGGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGG42200.4279298235651422No Hit
TAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGA41340.4192089788195018No Hit
ACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCA40560.411299375445549No Hit
GAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAAT39890.40450522895766644No Hit
AACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATC39600.40156447898529923No Hit
GACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGC35980.36485580691644115No Hit
GAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCC34220.3470084967393167No Hit
CTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTACT34120.34599444502470733No Hit
CTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTT33700.34173542782334815No Hit
CCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTA30940.3137476005001303No Hit
CCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGA29780.30198460061066196No Hit
GTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGG28730.29133705760726386No Hit
CCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTAC27700.2808923249467877No Hit
CCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCC27460.27845860083172524No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA26190.2655801440561866No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA25180.25533822173863224No Hit
CTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTTGGTTCCGGTATAT24780.25128201488019486No Hit
GGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAG24170.24509629942107788No Hit
GTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAG23970.24306819599185917No Hit
ATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAG22430.2274517995868753No Hit
TCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTT22200.22511948064327386No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT21280.2157902048688679No Hit
CCCCACAAGGGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTG20900.21193680835335243No Hit
CTCCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCAC20490.2077791963234541No Hit
CCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCA20320.20605530840861822No Hit
GGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCCAGATTCA19660.19936256709219657No Hit
TAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGG18980.19246701543285305No Hit
TACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAA18370.18628129997373605No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT18320.18577427411643138No Hit
AGCGAAAGCAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCC18210.18465881723036112No Hit
CTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTG17940.1819208776009159No Hit
GGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTA17760.18009558451461907No Hit
GGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACC17660.17908153280000974No Hit
GGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTGCAGAGAGAG17650.17898012762854879No Hit
GAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAG16840.17076630874021315No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16360.1658988605100883No Hit
CTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCC16130.1635665415664868No Hit
CATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTG15370.1558597485354558No Hit
AATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTT14780.1498768434192607No Hit
AAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCCAGATT14670.14876138653319043No Hit
ATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCC14660.14865998136172948No Hit
ACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTTGGTTCCGG14440.14642906758958893No Hit
CCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCC14120.14318410210283905No Hit
GTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCAC14040.14237286073115157No Hit
GGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGA14040.14237286073115157No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT13810.1400405417875501No Hit
GGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCT13620.13811384352979234No Hit
TAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTC13550.13740400732956579No Hit
CCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCT13450.13638995561495645No Hit
CCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCC13290.1347674728715815No Hit
GGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAG13080.1326379642709019No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT13060.13243515392798003No Hit
AGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCC12850.1303056453273004No Hit
GGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTC12650.12827754189808172No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC12420.12594522295448024No Hit
CCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTC12120.1229030678106522No Hit
CTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCT11910.1207735592099726No Hit
AGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGA11770.11935388680951951No Hit
AATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAA11360.11519627477962122No Hit
CAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTAT11350.11509486960816026No Hit
ACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTC11070.11225552480725412No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC10760.10911196449196515No Hit
GTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTC10750.10901055932050423No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10650.10799650760589488No Hit
AAGCAGGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGA10500.10647543003398088No Hit
CATTAATAGAGGTGGAGCGTGCAGAGAGAGGGAGGTGACGGTCCCAGTCT10490.10637402486251993No Hit
CCACAAGGGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTGCA10490.10637402486251993No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC10370.10515716280498873No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10260.10404170591891844No Hit
CTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTC10160.10302765420430911No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)