FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A11a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_A11a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752680
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG43600.579263432002976No Hit
ATCTAAGACTGTCTCTCTTTTAATCACACACACACATGCACACACACAGA26430.35114524100547373No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT25410.3375936653026519No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT24990.33201360471913693No Hit
ATATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGA24660.3276292714035181No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA18190.2416697667003242No Hit
GTCTTAGATTCTCCTTCATGAGTATGTCCCTTAGAGGGATAAATGATGAA15990.21244087792953179No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC15330.2036722112982941No Hit
CAATAATGGTCAACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACA14620.19423925174044748No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG14600.1939735345698039No Hit
ACTTAGGACTGGGCTATACATCCAACAGCAGCAGCACTATGGGTCAAAAT14310.19012063559547218No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC14150.18799489823032364No Hit
ACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACATCAGGAAAGACT13960.18547058510920975No Hit
GTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCACTTCCTCTCTGCC13530.17975766594037307No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT13350.17736621140458095No Hit
ACCTACAACAGTCAAAACACTGCTTACAACTTAGGACTGGGCTATACATC13210.175506191210076No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC13170.17497475686878886No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC13010.17284901950364034No Hit
ATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGAAA12910.1715204336504225No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT12760.16952755487059573No Hit
TTATTGTTCTTTCTCCCCAAAGTAGTCTTTCCTGATGTCTGACTGCTCCT12460.16554179731094223No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA12250.16275176701918478No Hit
GTTCTGGAGACTGAATAGGAAAACCAGAAAAGAGAACATATCAGGCAGAG11880.1578359993622788No Hit
CTCCAGAACCCATCCACATGCCGGTTCTCTGAGTTCCAGAATTTCCCCAG11610.15424881755859063No Hit
ATCTTAAGGTTAGCTGATTGACAACCTTAACTCCATCTTTGACCTAATAC11460.15225593877876387No Hit
GTAGGAGACAGAGTGGCATTTTGTACAATATTAAATTCAGGTTCTCAATA11460.15225593877876387No Hit
GAATAATGTCCCTCCCCAAAAATGATAGCCCAATTCCTAGAAACTGTGAA11440.1519902216081203No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11400.15145878726683318No Hit
CTCTAAGGGACATACTCATGAAGGAGAATCTAAGACTGTCTCTCTTTTAA11360.15092735292554604No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA11180.14853589838975395No Hit
ATCTTTTATTGCTTATTTGGTCACCACTTCCTCTCTGCCTGATATGTTCT11150.1481373226337886No Hit
CTTAATGACTGAGCCACTCAAGTGCCAACTGTGGCATACCTGTGAGAGCA11150.1481373226337886No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT11060.14694159536589255No Hit
ATAGAGAATGGGCTTACCATGATCCGATTAAACAAACATGACTTACTCCT11030.1465430196099272No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG11010.1462773024392836No Hit
CATCTGGATGTATAAAAAAATGCTCATGATCTCAACAGAGGAATGCTCTT10920.14508157517138756No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG10820.14375298931816974No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT10710.1422915448796301No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT10690.14202582770898656No Hit
CTCTACGGAAAGCTTACATTGCCTCTTGTGAGTGTATCCAATCTCTAGAA10660.14162725195302123No Hit
CAATAGAGAATGGGCTTACCATGATCCGATTAAACAAACATGACTTACTC10620.14109581761173406No Hit
CTCTAGAACTTTCTCATCTTGCAAAACTGAAATTCTGTAACTCCCCATGA10470.1391029388319073No Hit
CATATGAACAAGTACTGAATTTTGTCTATGCTCAAATGAGGTGATGTGTG10300.13684434288143701No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA10270.13644576712547166No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA10110.1343200297603231No Hit
TTGTACACCTGTGTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGG10020.13312430249242704No Hit
GTCCTGGGGAGACACCAAACTGTGTTTTAGTGAGCTTCCTAAGGCTTGAA9990.1327257267364617No Hit
GAACTATCCTGATTGATCCTCTAAGCAGACGAGAGGTTACCTGAGGTTTT9830.13059998937131317No Hit
GTCTAGGGAATATCCTCTAAGGAGTAAGTCATGTTTGTTTAATCGGATCA9810.1303342722006696No Hit
AGTTAGGGTTGCTTTGGGCTCCCAGATCACCAACAGATTTCATCATTTAT9610.12767710049423395No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT9570.12714566615294678No Hit
GTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGGCACACCCCAAGT9480.12594993888505074No Hit
ATCCTGATTGATCCTCTAAGCAGACGAGAGGTTACCTGAGGTTTTCAGTA9470.12581708029972896No Hit
AAGTAAGATATGCCAGTTGCGAAAAGAAATACTTTGTGATTCTACTTAAC9390.12475421161715469No Hit
GAGTGATACAATCCCACCTGCGTGTTAGAAAGGATTTTCAAGTGAAGGTT9240.12276133283732796No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA9060.12036987830153585No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT9020.1198384439602487No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA8980.11930700961896158No Hit
GTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATAGAATGATGGTTA8960.11904129244831801No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA8880.11797842376574375No Hit
AGACTATACTCTAAGTGGTATTAGGTCAAAGATGGAGTTAAGGTTGTCAA8870.11784556518042197No Hit
TAATGGTGGTGTGTCCCTACTTTTAGATAATAACGCACAATAGAGAATGG8850.11757984800977839No Hit
ATATATAGACTCTCTGTGGACTTCAGAGTCAAAACCAGCTATTAACTCGG8700.11558696922995164No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC8660.1150555348886645No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT8650.11492267630334271No Hit
TATATAGACTCTCTGTGGACTTCAGAGTCAAAACCAGCTATTAACTCGGC8470.11253122176755062No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT8450.11226550459690705No Hit
CTTCTACCCTGTAAGTATCTCTCCGTTCATTGCTGTTAACTGTCCCCCAG8450.11226550459690705No Hit
GAACAATAATCACCTGATGTAAAAATCCAGTGACAACACTGACAAGCAAG8390.11146835308497635No Hit
ATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAAT8260.10974119147579317No Hit
CATCAGGAAAGACTACTTTGGGGAGAAAGAACAATAATCACCTGATGTAA8250.1096083328904714No Hit
CTTCAACTCAGTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCACTT8220.10920975713450602No Hit
GCCTGATATGTTCTCTTTTCTGGTTTTCCTATTCAGTCTCCAGAACCCAT8200.10894403996386245No Hit
TTCCTATTCAGTCTCCAGAACCCATCCACATGCCGGTTCTCTGAGTTCCA8170.10854546420789712No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT8090.10748259552532284No Hit
AGATTGTACCCATGAAAAAGGAGCAGTCAGACATCAGGAAAGACTACTTT8080.10734973694000106No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG8040.10681830259871392No Hit
ATAATCACCTGATGTAAAAATCCAGTGACAACACTGACAAGCAAGTTGAT7800.10362969655099113No Hit
TCCTTCAACTCAGTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCAC7770.10323112079502576No Hit
GGGTATGCTGGAAGCATTTTTATTTCAGTTTAGTATCCATTTCGGGTAGG7680.10203539352712972No Hit
CTCTAGGCACCTTGTGTTAAGTAGAATCACAAAGTATTTCTTTTCGCAAC7630.1013711006005208No Hit
CTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGA7620.10123824201519903No Hit
GTAGTTGGGTGTTTGCTACTTGCACTGAACTATCCTGATTGATCCTCTAA7620.10123824201519903No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT7610.10110538342987725No Hit
GTATTAGGTCAAAGATGGAGTTAAGGTTGTCAATCAGCTAACCTTAAGAT7550.10030823191794654No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)