FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_F4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATGGCCCAGTATCTGCTTCTCAGTTCCAGGGTATTGGAGTCCATGGTT12.941176470588235No Hit
GAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGG12.941176470588235No Hit
ATTAATAAACTTATTTAAAGCAGTAATGAAAGTGATTCAGTTTAGAAGCT12.941176470588235No Hit
GAAAGCAGGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGG12.941176470588235No Hit
ACTCTCTTCTTTTAAATTAAAACACTTGAAAAGTAATGTTTTTTTCTCTT12.941176470588235No Hit
GCCTATCTGCGTACATACATCGATAGTATTCTGAAAGCAATGGGGATCTG12.941176470588235No Hit
GGGGGGTGATAGAGACTTTGTTGGTCAGCACTAGTAGGTGGACTGTCTCT12.941176470588235No Hit
GGGGTGTTCGCATGTCGGATGTCCGTCCTTCATTGTTCCCGCTGAATGCT12.941176470588235No Hit
ATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATA12.941176470588235No Hit
AATAAATACGTGCTGTTATTACTATGGTGCAAAAATCATCTTGCTGGGTG12.941176470588235No Hit
GGCTGGAACAGGTTGCGCCATATTTCTGGAAAACCACCCCTTTTACAGGA12.941176470588235No Hit
AAATACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGCCTGAGTCCAT12.941176470588235No Hit
CCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTCAGTTCATGACC12.941176470588235No Hit
GGGGTACGGGGACTGTGACCCCCAAGGAAGAGGCAGATTTGGGGTCAAGC12.941176470588235No Hit
ATACTGGGCCATAAGGACCAGGAGTGGAGGAAATACCAATCAACAAAAGG12.941176470588235No Hit
ATATACAACAGGATGGGAACAGTGAACACAGAAGCTGCTTTTGGTCTAGT12.941176470588235No Hit
GCCGGATGCAGGGCTTGCTCCCAGGACCCTGGCATCAACCCCCTGTCTAT12.941176470588235No Hit
AGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACA12.941176470588235No Hit
GCTGGAAGCATATGTGGGCTAGGGCGGTAGTGTGGTACGGGCAGGGCCGA12.941176470588235No Hit
GGTACACTCCCAACAGAAGTCATAAAAGAGGAAGAAAAACCTTTGAAAAG12.941176470588235No Hit
AGTAGAAACAAGGGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACA12.941176470588235No Hit
GAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCCAGCGAATCTCTGTA12.941176470588235No Hit
GGGTTGGATGTAGAATCTCCCGATTCCTCCAATCATTCTTCCGACAGATG12.941176470588235No Hit
GAGGGGGAGGGAGAAGCAGGCTCCCCCCTGAGTAAGGCTGTCTCTTATAC12.941176470588235No Hit
TACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTG12.941176470588235No Hit
GCTGGGGAAGGATGGGCGTGGCTGACCGGACAGATGGTTCTGTCTCTTAT12.941176470588235No Hit
GATGATTTTTGCACCATAGTAATAACAGCACGTATTTATTAAGGGACTAC12.941176470588235No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC12.941176470588235No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG12.941176470588235No Hit
GTGGCTCGAGTCCTGAAGGGCAGATAGCCAGTGAATTACTTAGTGGTGTG12.941176470588235No Hit
CACATAGGCACCCCAAGATTAATCCATTTTAGGCATCCTAGATTGAGAAA12.941176470588235No Hit
GGGTGGAAGATCCCAATGATATTTGCAGCAAGGCCGAGAGGATCAGTTGA12.941176470588235No Hit
TGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGTTGCAACT12.941176470588235No Hit
ACTTCTCCCCTTTGCTGCTGCCCCACCAGAACCTGTCTCTTATACACATC12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)