FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGAGCAAAGTGTCCTAATCTCAACTGGGGTCTATTTATAGTAGAA12.941176470588235No Hit
GTGCCATAGGGGAGACACACAAATTCATACGAGAAGATAAGTTGAGTTAA12.941176470588235No Hit
ATAAAAAGCATAGGTCAAGAAACAAGAAAAATATTATAGATTTCCCTTTA12.941176470588235No Hit
GGGTTATGTGTTCTCAAATCTTCTCTGGAGAGTGAAAGCTTTTAATAGGT12.941176470588235No Hit
GCTATGGATGTAGTGAAGTTATAAAATTAGCATCACATTTTCTACAATCG12.941176470588235No Hit
CCCAAGAAATTACCAAATTTTTTATAAACTCGTTCTAGCACTTTACATTA12.941176470588235No Hit
TGGATAAATAAGTGATAATTAACGATTACTTTAAAATAGTCGAGAATGTA12.941176470588235No Hit
GGATAAAATTTTTTTATATGTTGACCATGTAATTTTAAAACGTTCAAAGT12.941176470588235No Hit
CCATTCAAGTCATCCGATGAGGACCCAATCTGCATTTTTGACATCCTCAT12.941176470588235No Hit
GTGTTTGGATAGGGAGATCTAAATGCATTAGTTCAAGAAGCGGTTTTGAG12.941176470588235No Hit
TTACTGATCACTTCAAAATTAACTTCAGTAAGTATGCTGAGAATGTATTC12.941176470588235No Hit
GTGTACAAGGTCACCAATCAGCTGATGGTGTTCTATTTGTTGCCACTTGG12.941176470588235No Hit
CGGTAAGTCGTTTTGTTTACTTTTTGTATCTCATTGCCATCATCCACTTC12.941176470588235No Hit
CATTTATGCAAGCCTTACAACTACTGATTGAAGTATAACAAGAGATAAGA12.941176470588235No Hit
GATCACAGTCTTGCATAAATGAAGTTTCGAAACAGCTATATTTTGAGAAT12.941176470588235No Hit
TTCAAGATCCATTCCACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGAT12.941176470588235No Hit
GCCCTGGAGACTCTGCGGTCTCAGCTGCCCCTAGTGGCTCGTCGGTGCAT12.941176470588235No Hit
ATAAAACTCTTACTTATATTCTTAAGCCAGGTTTTCTTACTTTCTTTCTT12.941176470588235No Hit
TCATTGGGATCTTGCACCTGATATTGTGGATTACCTGTCTCTTATACACA12.941176470588235No Hit
TTCATCGACAGTTTTTCCACATAAGATAACAACTTTCAAAATCATCCATT12.941176470588235No Hit
AACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACCTGTCTCTTAT12.941176470588235No Hit
GCCCCAGGGAAGTGACCACGACACCCGCGTAGGAGGCTGCTGGACATATT12.941176470588235No Hit
ATCTTGTTCTACTTCAAGCTGTACTACAAACGCTTCCACAAAAGTAACAT12.941176470588235No Hit
CGCTGGGTCTGTGCTTGAACCTCTGCTTGTGCATGTTGTCGAGGAAGATA12.941176470588235No Hit
CTACTTCAGTGCATGTGTGAGGAAGGAGTTGCACCAAGATGCATTAAGCA12.941176470588235No Hit
GCGTAAAAGTTAATTGTACGAAACAATACTCTGACGCAATCAATCTATGA12.941176470588235No Hit
CATTTTTGAAACATAGGTAATAGATAGAGTGGTTTACTTAATATATAAAG12.941176470588235No Hit
AAACTAACAAGTTTGTTGCAATATGCACACATTTGGAAGTTTAATCTCTA12.941176470588235No Hit
ACATATAGGAGACCGGTTGGAATATCTAGCATCGTAGAGGCCATGGTGTC12.941176470588235No Hit
AAATATATGTATGCGTACTAAAAGACTCTAAGGAGTATAACTAACTGACA12.941176470588235No Hit
GTTTCGATATCGAGGCCAAGGGTGTTGCCTCTTCCTTTTAAGGACTTTTG12.941176470588235No Hit
CTTTTACCCCTCCCTGACTTCTTTACAACACATAACGTGACAGCTGTCTC12.941176470588235No Hit
GCATGATTGTCAGAAAATATGATCAATATGATTTTATTCTTCTTAAATTT12.941176470588235No Hit
GTTCGAGTCTCTCAAAATATACAGAGATTCGCTTCGAGCTGTCTCTAATA12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)