FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R1_A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAGTGTAGAATATGTATTTAACATTAGGATTTCAGAAGCATGAGAAA12.7777777777777777No Hit
AGTAGAAACAAGGGAGTTTTTTAAAATAAAAGAAGCTTAACCAAAACACC12.7777777777777777No Hit
GAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGCCCCATGCA12.7777777777777777No Hit
CATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCTCAGTCGTTGGGCGTCTG12.7777777777777777No Hit
GTGCTCTCTAATGCAAGGTTCAACACTTCCCAGAATGTAGGGTGCCGCAG12.7777777777777777No Hit
ACCAAATCCAGAATTAAAATAGCACAATAAGTTTGCCAAATGCAAAAAAT12.7777777777777777No Hit
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA12.7777777777777777No Hit
GTGCATTTAAAATATGACTCGGAGTTTTGTATACACAACATTAATAATAA12.7777777777777777No Hit
CTATCATCCCGTCAGGCCCCCTCACGGCCGAGATCGCGCACAGACTGGAA12.7777777777777777No Hit
CGCAAGCGATTTGCAGACAATGGATTGGGTGATGACCCATTCCTTGATCG12.7777777777777777No Hit
TTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTAATGCTAGGGCAG12.7777777777777777No Hit
TCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGG12.7777777777777777No Hit
GGAGAAACAAGTGCGTTTTTAAACAAGTCTACACTAATTGATTGACATCC12.7777777777777777No Hit
CCACATAAGAATGTCCTGGAAGAGAAGGTAATGTTGAAATTTCTCCAAAT12.7777777777777777No Hit
GGATATTCCTCAATCCTGTGGAAAGGCTCAATTTTGTGATGTTTACATAT12.7777777777777777No Hit
GACACATTATGTATAGGTTATCATGAGAACAATTCAACAGACACTGTAGA12.7777777777777777No Hit
CTTTGTAATCATACAAATCAGGAAGAAATTTAGGCTTCTCTACCCCTGTT12.7777777777777777No Hit
CTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCACAG12.7777777777777777No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC12.7777777777777777No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGAC12.7777777777777777No Hit
CTTCTCCACCTGCTGTGTCATCATGTATTCCATCACCACCAGCACCATGG12.7777777777777777No Hit
CCATATGGCCATAATCAAAAAGAACACATCAGGAAGGAAAGAGAAGAACC12.7777777777777777No Hit
TCGCTCGACTGTAAGCTCCATAAAGGTAGGAAGCGCCTTCTGTCTTTCCC12.7777777777777777No Hit
ATACAGAGATTCGCTAGGAGAATCTCTGATGAGAAACGGCTGTCTCATAA12.7777777777777777No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA12.7777777777777777No Hit
CAAGTGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACTGTCTC12.7777777777777777No Hit
GAGTGGTTGTATTAGCCATCTGTCTTTGATCACTATGCTGTGAAGAAGCA12.7777777777777777No Hit
ATATACAACAGTAGGCGAAAAGTGAAACAATCATCTGCTGATTTAATACT12.7777777777777777No Hit
GTTGTAAGGCTTGCATAAATGATATTTGTTCGAAACAATTCTCTGTCGCT12.7777777777777777No Hit
GCTAGGTCTTGACATTCGCCACTTGATCTGCTCCGATCGATCTGTGATCT12.7777777777777777No Hit
GCTATATACATTTGCAACCGCAACTGCAGACACATTATGTATAGGTTATC12.7777777777777777No Hit
ACATAGTACTGAAAGAGAACTTCAGTGTAATCTTTAACCGATTAGATACC12.7777777777777777No Hit
CCTTATTTCTTCTTTGTCATAAAGGATGAGTTCTCTCATCCACTTTCCGT12.7777777777777777No Hit
TAATCATTATAACATGATAATGAATCAGAATGATAAGTATATTAATTAAT12.7777777777777777No Hit
ATTCTACATCCAAATGTGCACTGAACTCAAACTCAGTGATTATGAGGGAC12.7777777777777777No Hit
GTACTGAAATCGAACTTCAGTGTAATATTTAAAAGATTAGAGAACTTGAT12.7777777777777777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)