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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.9

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2023-07-11, 07:45 based on data in: /scratch/gencore/logs/html/H7GWFDSX7/merged


        General Statistics

        Showing 386/386 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        H7GWFDSX7_n01_C.4_POOLRET74
        22.6%
        40%
        66.4
        H7GWFDSX7_n01_C000_POOLRET74
        20.4%
        41%
        60.6
        H7GWFDSX7_n01_C013_POOLRET74
        32.3%
        47%
        77.7
        H7GWFDSX7_n01_C014_POOLRET74
        25.8%
        43%
        70.0
        H7GWFDSX7_n01_C021_POOLRET74
        38.6%
        45%
        52.9
        H7GWFDSX7_n01_C024_POOLRET74
        31.6%
        45%
        55.0
        H7GWFDSX7_n01_C027_POOLRET74
        20.8%
        42%
        57.7
        H7GWFDSX7_n01_C028_POOLRET74
        43.0%
        46%
        51.7
        H7GWFDSX7_n01_C029_POOLRET74
        30.8%
        44%
        60.4
        H7GWFDSX7_n01_C033_POOLRET74
        33.5%
        45%
        45.6
        H7GWFDSX7_n01_C034_POOLRET74
        30.0%
        45%
        66.5
        H7GWFDSX7_n01_C040_POOLRET74
        25.4%
        43%
        48.4
        H7GWFDSX7_n01_C043_POOLRET74
        22.2%
        40%
        63.0
        H7GWFDSX7_n01_C047_POOLRET74
        30.9%
        44%
        66.3
        H7GWFDSX7_n01_C049_POOLRET74
        30.4%
        45%
        48.1
        H7GWFDSX7_n01_C055_POOLRET74
        30.1%
        44%
        54.0
        H7GWFDSX7_n01_C056_POOLRET74
        30.5%
        43%
        68.1
        H7GWFDSX7_n01_C058_POOLRET74
        37.2%
        45%
        49.2
        H7GWFDSX7_n01_C061_POOLRET74
        21.7%
        40%
        61.3
        H7GWFDSX7_n01_C063_POOLRET74
        42.0%
        45%
        64.6
        H7GWFDSX7_n01_C065_POOLRET74
        32.5%
        45%
        41.2
        H7GWFDSX7_n01_C066_POOLRET74
        35.1%
        45%
        62.3
        H7GWFDSX7_n01_C069_POOLRET74
        42.5%
        46%
        46.9
        H7GWFDSX7_n01_C072_POOLRET74
        44.2%
        46%
        68.2
        H7GWFDSX7_n01_C075_POOLRET74
        20.3%
        40%
        55.6
        H7GWFDSX7_n01_C077_POOLRET74
        35.1%
        45%
        47.0
        H7GWFDSX7_n01_C083_POOLRET74
        30.5%
        44%
        50.9
        H7GWFDSX7_n01_C085_POOLRET74
        29.3%
        42%
        68.7
        H7GWFDSX7_n01_C087_POOLRET74
        34.0%
        45%
        37.1
        H7GWFDSX7_n01_C088_POOLRET74
        45.0%
        46%
        56.7
        H7GWFDSX7_n01_C092_POOLRET74
        34.9%
        44%
        50.1
        H7GWFDSX7_n01_C096_POOLRET74
        20.9%
        39%
        56.3
        H7GWFDSX7_n01_C097_POOLRET74
        33.8%
        43%
        81.8
        H7GWFDSX7_n01_CB4856_BaerAncestor_POOLRET74_a
        19.9%
        42%
        56.8
        H7GWFDSX7_n01_CB4856_BaerAncestor_POOLRET74_b
        26.6%
        40%
        84.1
        H7GWFDSX7_n01_CB4856_BaerAncestor_POOLRET74_c
        24.0%
        40%
        73.3
        H7GWFDSX7_n01_E.1_POOLRET74
        20.0%
        40%
        50.9
        H7GWFDSX7_n01_E.4_POOLRET74
        23.0%
        40%
        71.4
        H7GWFDSX7_n01_E405_POOLRET74
        25.0%
        40%
        69.9
        H7GWFDSX7_n01_E409_POOLRET74
        60.5%
        44%
        441.6
        H7GWFDSX7_n01_E412_POOLRET74
        20.0%
        42%
        38.4
        H7GWFDSX7_n01_E413_POOLRET74
        19.2%
        41%
        45.3
        H7GWFDSX7_n01_E423_POOLRET74
        23.6%
        42%
        73.8
        H7GWFDSX7_n01_E427_POOLRET74
        19.6%
        40%
        51.2
        H7GWFDSX7_n01_E428_POOLRET74
        29.7%
        43%
        78.1
        H7GWFDSX7_n01_E443_POOLRET74
        22.9%
        43%
        65.0
        H7GWFDSX7_n01_E446_POOLRET74
        23.2%
        43%
        62.5
        H7GWFDSX7_n01_E449_POOLRET74
        23.3%
        42%
        44.9
        H7GWFDSX7_n01_E450_POOLRET74
        39.2%
        46%
        48.5
        H7GWFDSX7_n01_E452_POOLRET74
        29.3%
        45%
        48.8
        H7GWFDSX7_n01_E453_POOLRET74
        19.9%
        39%
        52.5
        H7GWFDSX7_n01_E454_POOLRET74
        37.4%
        46%
        46.9
        H7GWFDSX7_n01_E455_POOLRET74
        22.0%
        41%
        58.6
        H7GWFDSX7_n01_E458_POOLRET74
        27.1%
        44%
        46.9
        H7GWFDSX7_n01_E459_POOLRET74
        19.5%
        43%
        56.9
        H7GWFDSX7_n01_E460_POOLRET74
        26.3%
        43%
        48.1
        H7GWFDSX7_n01_E461_POOLRET74
        24.2%
        42%
        46.4
        H7GWFDSX7_n01_E462_POOLRET74
        22.8%
        41%
        57.0
        H7GWFDSX7_n01_E463_POOLRET74
        31.9%
        43%
        53.7
        H7GWFDSX7_n01_E464_POOLRET74
        21.2%
        40%
        58.4
        H7GWFDSX7_n01_E466_POOLRET74
        34.7%
        44%
        49.1
        H7GWFDSX7_n01_E472_POOLRET74
        28.2%
        42%
        75.6
        H7GWFDSX7_n01_E477_POOLRET74
        32.2%
        45%
        63.0
        H7GWFDSX7_n01_E478_POOLRET74
        20.9%
        41%
        58.3
        H7GWFDSX7_n01_E479_POOLRET74
        16.4%
        40%
        35.1
        H7GWFDSX7_n01_E480_POOLRET74
        23.8%
        43%
        47.5
        H7GWFDSX7_n01_E484_POOLRET74
        20.6%
        43%
        44.4
        H7GWFDSX7_n01_E487_POOLRET74
        24.6%
        46%
        31.1
        H7GWFDSX7_n01_E494_POOLRET74
        33.8%
        44%
        58.7
        H7GWFDSX7_n01_E498_POOLRET74
        22.9%
        41%
        67.5
        H7GWFDSX7_n01_ECA2936_POOLRET74
        23.2%
        41%
        72.3
        H7GWFDSX7_n01_ECA2939_POOLRET74
        19.3%
        47%
        0.1
        H7GWFDSX7_n01_ECA2945_POOLRET74
        21.6%
        40%
        64.5
        H7GWFDSX7_n01_ECA2949_POOLRET74
        18.9%
        42%
        60.7
        H7GWFDSX7_n01_ECA2956_POOLRET74
        22.5%
        39%
        68.7
        H7GWFDSX7_n01_ECA2963_POOLRET74
        23.0%
        40%
        70.2
        H7GWFDSX7_n01_ECA2964_POOLRET74
        18.4%
        42%
        52.7
        H7GWFDSX7_n01_ECA2967_POOLRET74
        19.6%
        40%
        53.6
        H7GWFDSX7_n01_ECA2968_POOLRET74
        18.2%
        40%
        51.9
        H7GWFDSX7_n01_ECA2970_POOLRET74
        22.1%
        43%
        73.5
        H7GWFDSX7_n01_ECA2974_POOLRET74
        20.6%
        40%
        55.5
        H7GWFDSX7_n01_ECA2975_POOLRET74
        24.3%
        43%
        83.1
        H7GWFDSX7_n01_ECA2983_POOLRET74
        23.6%
        41%
        74.0
        H7GWFDSX7_n01_ECA3006_POOLRET74
        24.5%
        40%
        74.6
        H7GWFDSX7_n01_ECA3037_POOLRET74
        20.6%
        42%
        59.7
        H7GWFDSX7_n01_ECA3045_POOLRET74
        16.8%
        42%
        45.8
        H7GWFDSX7_n01_ECA3049_POOLRET74
        23.0%
        41%
        71.0
        H7GWFDSX7_n01_ECA3053_POOLRET74
        21.2%
        42%
        54.5
        H7GWFDSX7_n01_ECA3073_POOLRET74
        18.1%
        41%
        51.5
        H7GWFDSX7_n01_ECA3077_POOLRET74
        20.7%
        42%
        63.5
        H7GWFDSX7_n01_ECA3085_POOLRET74
        25.3%
        42%
        79.7
        H7GWFDSX7_n01_ECA3087_POOLRET74
        18.6%
        43%
        66.6
        H7GWFDSX7_n01_ECA3088_POOLRET74
        22.6%
        40%
        71.9
        H7GWFDSX7_n01_ECA3108_POOLRET74
        26.3%
        41%
        86.6
        H7GWFDSX7_n01_ECA3110_POOLRET74
        18.5%
        41%
        57.2
        H7GWFDSX7_n01_ECA3111_POOLRET74
        17.3%
        44%
        46.1
        H7GWFDSX7_n01_ECA3112_POOLRET74
        26.1%
        40%
        83.7
        H7GWFDSX7_n01_ECA3113_POOLRET74
        24.1%
        43%
        75.2
        H7GWFDSX7_n01_ECA3114_POOLRET74
        23.0%
        41%
        75.4
        H7GWFDSX7_n01_ECA3115_POOLRET74
        25.4%
        40%
        79.7
        H7GWFDSX7_n01_ECA3116_POOLRET74
        25.3%
        42%
        88.2
        H7GWFDSX7_n01_ECA3117_POOLRET74
        23.3%
        40%
        68.9
        H7GWFDSX7_n01_ECA3118_POOLRET74
        18.8%
        41%
        51.9
        H7GWFDSX7_n01_ECA3119_POOLRET74
        24.6%
        40%
        80.4
        H7GWFDSX7_n01_EG8072_BaerAncestor_POOLRET74_a
        26.0%
        41%
        86.4
        H7GWFDSX7_n01_EG8072_BaerAncestor_POOLRET74_b
        18.8%
        42%
        52.3
        H7GWFDSX7_n01_EG8072_BaerAncestor_POOLRET74_c
        20.8%
        41%
        60.3
        H7GWFDSX7_n01_G.3_POOLRET74
        22.2%
        39%
        63.8
        H7GWFDSX7_n01_G200_POOLRET74
        24.2%
        42%
        51.7
        H7GWFDSX7_n01_G205_POOLRET74
        34.8%
        45%
        51.2
        H7GWFDSX7_n01_G207_POOLRET74
        28.8%
        45%
        54.9
        H7GWFDSX7_n01_G208_POOLRET74
        22.8%
        43%
        49.6
        H7GWFDSX7_n01_G220_POOLRET74
        22.6%
        41%
        64.4
        H7GWFDSX7_n01_G225_POOLRET74
        29.6%
        43%
        62.7
        H7GWFDSX7_n01_G228_POOLRET74
        35.1%
        45%
        57.8
        H7GWFDSX7_n01_G236_POOLRET74
        19.7%
        45%
        50.4
        H7GWFDSX7_n01_G238_POOLRET74
        21.4%
        42%
        60.7
        H7GWFDSX7_n01_G240_POOLRET74
        19.9%
        42%
        39.0
        H7GWFDSX7_n01_G243_POOLRET74
        25.5%
        41%
        83.4
        H7GWFDSX7_n01_G246_POOLRET74
        22.8%
        40%
        66.8
        H7GWFDSX7_n01_G250_POOLRET74
        22.9%
        42%
        59.1
        H7GWFDSX7_n01_G251_POOLRET74
        32.7%
        46%
        47.3
        H7GWFDSX7_n01_G256_POOLRET74
        27.8%
        42%
        61.4
        H7GWFDSX7_n01_G260_POOLRET74
        28.2%
        42%
        61.0
        H7GWFDSX7_n01_G263_POOLRET74
        29.9%
        43%
        54.7
        H7GWFDSX7_n01_G267_POOLRET74
        35.4%
        43%
        89.3
        H7GWFDSX7_n01_G272_POOLRET74
        40.1%
        53%
        66.2
        H7GWFDSX7_n01_G275_POOLRET74
        17.1%
        39%
        46.4
        H7GWFDSX7_n01_G276_POOLRET74
        24.5%
        42%
        65.0
        H7GWFDSX7_n01_G278_POOLRET74
        42.9%
        46%
        52.2
        H7GWFDSX7_n01_G288_POOLRET74
        32.0%
        43%
        65.2
        H7GWFDSX7_n01_G291_POOLRET74
        28.6%
        43%
        48.5
        H7GWFDSX7_n01_G292_POOLRET74
        29.5%
        43%
        62.1
        H7GWFDSX7_n01_G293_POOLRET74
        21.7%
        42%
        47.1
        H7GWFDSX7_n01_G294_POOLRET74
        29.1%
        44%
        34.5
        H7GWFDSX7_n01_J.5_POOLRET74
        26.2%
        41%
        75.1
        H7GWFDSX7_n01_J301_POOLRET74
        48.3%
        47%
        45.1
        H7GWFDSX7_n01_J302_POOLRET74
        62.4%
        50%
        61.9
        H7GWFDSX7_n01_J312_POOLRET74
        25.4%
        39%
        83.1
        H7GWFDSX7_n01_J314_POOLRET74
        20.5%
        40%
        56.0
        H7GWFDSX7_n01_J317_POOLRET74
        38.7%
        45%
        53.1
        H7GWFDSX7_n01_J318_POOLRET74
        36.7%
        45%
        56.4
        H7GWFDSX7_n01_J322_POOLRET74
        19.1%
        43%
        27.8
        H7GWFDSX7_n01_J325_POOLRET74
        38.9%
        45%
        62.0
        H7GWFDSX7_n01_J330_POOLRET74
        28.5%
        43%
        67.4
        H7GWFDSX7_n01_J332_POOLRET74
        36.1%
        45%
        52.2
        H7GWFDSX7_n01_J336_POOLRET74
        37.6%
        44%
        68.1
        H7GWFDSX7_n01_J337_POOLRET74
        19.8%
        39%
        48.5
        H7GWFDSX7_n01_J339_POOLRET74
        22.2%
        42%
        48.2
        H7GWFDSX7_n01_J347_POOLRET74
        33.1%
        46%
        58.4
        H7GWFDSX7_n01_J353_POOLRET74
        34.6%
        44%
        52.8
        H7GWFDSX7_n01_J356_POOLRET74
        43.4%
        47%
        39.5
        H7GWFDSX7_n01_J357_POOLRET74
        50.9%
        48%
        63.7
        H7GWFDSX7_n01_J361_POOLRET74
        46.6%
        46%
        60.4
        H7GWFDSX7_n01_J366_POOLRET74
        18.6%
        39%
        47.0
        H7GWFDSX7_n01_J368_POOLRET74
        41.2%
        46%
        54.0
        H7GWFDSX7_n01_J372_POOLRET74
        25.7%
        42%
        71.8
        H7GWFDSX7_n01_J373_POOLRET74
        22.3%
        40%
        59.6
        H7GWFDSX7_n01_J376_POOLRET74
        31.2%
        43%
        60.1
        H7GWFDSX7_n01_J382_POOLRET74
        51.3%
        49%
        74.2
        H7GWFDSX7_n01_J390_POOLRET74
        16.0%
        44%
        10.0
        H7GWFDSX7_n01_J391_POOLRET74
        48.4%
        47%
        63.1
        H7GWFDSX7_n01_J393_POOLRET74
        28.8%
        43%
        61.9
        H7GWFDSX7_n01_J394_POOLRET74
        38.7%
        48%
        41.8
        H7GWFDSX7_n01_J396_POOLRET74
        38.5%
        45%
        78.2
        H7GWFDSX7_n01_J397_POOLRET74
        44.8%
        46%
        60.1
        H7GWFDSX7_n01_JU360_BaerAncestor_POOLRET74_a
        24.4%
        39%
        67.6
        H7GWFDSX7_n01_JU360_BaerAncestor_POOLRET74_b
        26.6%
        42%
        83.5
        H7GWFDSX7_n01_JU360_BaerAncestor_POOLRET74_c
        21.7%
        40%
        57.4
        H7GWFDSX7_n01_M.4_POOLRET74
        17.8%
        39%
        41.9
        H7GWFDSX7_n01_M.5_POOLRET74
        24.0%
        40%
        77.0
        H7GWFDSX7_n01_M110_POOLRET74
        26.9%
        41%
        75.8
        H7GWFDSX7_n01_M114_POOLRET74
        24.9%
        41%
        80.5
        H7GWFDSX7_n01_M119_POOLRET74
        46.8%
        47%
        60.7
        H7GWFDSX7_n01_M126_POOLRET74
        22.0%
        42%
        54.8
        H7GWFDSX7_n01_M128_POOLRET74
        23.1%
        41%
        55.7
        H7GWFDSX7_n01_M132_POOLRET74
        15.6%
        40%
        37.4
        H7GWFDSX7_n01_M135_POOLRET74
        14.6%
        39%
        30.3
        H7GWFDSX7_n01_M137_POOLRET74
        23.0%
        45%
        65.0
        H7GWFDSX7_n01_M142_POOLRET74
        28.9%
        43%
        53.2
        H7GWFDSX7_n01_M149_POOLRET74
        31.3%
        43%
        59.0
        H7GWFDSX7_n01_M151_POOLRET74
        24.2%
        41%
        71.6
        H7GWFDSX7_n01_M169_POOLRET74
        47.4%
        46%
        70.8
        H7GWFDSX7_n01_M170_POOLRET74
        19.0%
        40%
        46.0
        H7GWFDSX7_n01_M172_POOLRET74
        31.8%
        45%
        42.0
        H7GWFDSX7_n01_M173_POOLRET74
        25.2%
        42%
        62.0
        H7GWFDSX7_n01_M175_POOLRET74
        27.2%
        42%
        57.6
        H7GWFDSX7_n01_M186_POOLRET74
        30.1%
        44%
        51.7
        H7GWFDSX7_n01_M192_POOLRET74
        21.6%
        40%
        60.4
        H7GWFDSX7_n01_MY16_BaerAncestor_POOLRET74_a
        25.1%
        41%
        78.2
        H7GWFDSX7_n01_MY16_BaerAncestor_POOLRET74_b
        28.0%
        39%
        89.2
        H7GWFDSX7_n01_MY16_BaerAncestor_POOLRET74_c
        25.5%
        40%
        77.6
        H7GWFDSX7_n01_undetermined
        52.0%
        42%
        981.6
        H7GWFDSX7_n02_C.4_POOLRET74
        22.7%
        40%
        66.4
        H7GWFDSX7_n02_C000_POOLRET74
        20.7%
        41%
        60.6
        H7GWFDSX7_n02_C013_POOLRET74
        33.5%
        47%
        77.7
        H7GWFDSX7_n02_C014_POOLRET74
        26.7%
        43%
        70.0
        H7GWFDSX7_n02_C021_POOLRET74
        39.6%
        45%
        52.9
        H7GWFDSX7_n02_C024_POOLRET74
        31.7%
        45%
        55.0
        H7GWFDSX7_n02_C027_POOLRET74
        21.4%
        42%
        57.7
        H7GWFDSX7_n02_C028_POOLRET74
        44.2%
        46%
        51.7
        H7GWFDSX7_n02_C029_POOLRET74
        32.2%
        44%
        60.4
        H7GWFDSX7_n02_C033_POOLRET74
        34.3%
        45%
        45.6
        H7GWFDSX7_n02_C034_POOLRET74
        30.5%
        44%
        66.5
        H7GWFDSX7_n02_C040_POOLRET74
        26.0%
        43%
        48.4
        H7GWFDSX7_n02_C043_POOLRET74
        22.2%
        40%
        63.0
        H7GWFDSX7_n02_C047_POOLRET74
        31.8%
        44%
        66.3
        H7GWFDSX7_n02_C049_POOLRET74
        31.0%
        44%
        48.1
        H7GWFDSX7_n02_C055_POOLRET74
        30.7%
        44%
        54.0
        H7GWFDSX7_n02_C056_POOLRET74
        31.1%
        43%
        68.1
        H7GWFDSX7_n02_C058_POOLRET74
        38.4%
        45%
        49.2
        H7GWFDSX7_n02_C061_POOLRET74
        22.2%
        40%
        61.3
        H7GWFDSX7_n02_C063_POOLRET74
        42.1%
        45%
        64.6
        H7GWFDSX7_n02_C065_POOLRET74
        33.2%
        45%
        41.2
        H7GWFDSX7_n02_C066_POOLRET74
        36.3%
        44%
        62.3
        H7GWFDSX7_n02_C069_POOLRET74
        43.5%
        46%
        46.9
        H7GWFDSX7_n02_C072_POOLRET74
        45.3%
        46%
        68.2
        H7GWFDSX7_n02_C075_POOLRET74
        20.8%
        40%
        55.6
        H7GWFDSX7_n02_C077_POOLRET74
        36.3%
        45%
        47.0
        H7GWFDSX7_n02_C083_POOLRET74
        31.1%
        44%
        50.9
        H7GWFDSX7_n02_C085_POOLRET74
        30.1%
        43%
        68.7
        H7GWFDSX7_n02_C087_POOLRET74
        34.9%
        45%
        37.1
        H7GWFDSX7_n02_C088_POOLRET74
        46.7%
        46%
        56.7
        H7GWFDSX7_n02_C092_POOLRET74
        35.8%
        44%
        50.1
        H7GWFDSX7_n02_C096_POOLRET74
        20.9%
        39%
        56.3
        H7GWFDSX7_n02_C097_POOLRET74
        34.6%
        43%
        81.8
        H7GWFDSX7_n02_CB4856_BaerAncestor_POOLRET74_a
        20.6%
        41%
        56.8
        H7GWFDSX7_n02_CB4856_BaerAncestor_POOLRET74_b
        26.9%
        40%
        84.1
        H7GWFDSX7_n02_CB4856_BaerAncestor_POOLRET74_c
        24.7%
        40%
        73.3
        H7GWFDSX7_n02_E.1_POOLRET74
        20.0%
        40%
        50.9
        H7GWFDSX7_n02_E.4_POOLRET74
        23.2%
        40%
        71.4
        H7GWFDSX7_n02_E405_POOLRET74
        25.2%
        41%
        69.9
        H7GWFDSX7_n02_E409_POOLRET74
        61.8%
        43%
        441.6
        H7GWFDSX7_n02_E412_POOLRET74
        20.5%
        42%
        38.4
        H7GWFDSX7_n02_E413_POOLRET74
        19.7%
        41%
        45.3
        H7GWFDSX7_n02_E423_POOLRET74
        24.3%
        42%
        73.8
        H7GWFDSX7_n02_E427_POOLRET74
        19.9%
        40%
        51.2
        H7GWFDSX7_n02_E428_POOLRET74
        30.4%
        43%
        78.1
        H7GWFDSX7_n02_E443_POOLRET74
        23.2%
        42%
        65.0
        H7GWFDSX7_n02_E446_POOLRET74
        23.7%
        43%
        62.5
        H7GWFDSX7_n02_E449_POOLRET74
        23.8%
        42%
        44.9
        H7GWFDSX7_n02_E450_POOLRET74
        40.0%
        46%
        48.5
        H7GWFDSX7_n02_E452_POOLRET74
        30.1%
        45%
        48.8
        H7GWFDSX7_n02_E453_POOLRET74
        20.2%
        39%
        52.5
        H7GWFDSX7_n02_E454_POOLRET74
        38.5%
        46%
        46.9
        H7GWFDSX7_n02_E455_POOLRET74
        22.6%
        41%
        58.6
        H7GWFDSX7_n02_E458_POOLRET74
        28.1%
        44%
        46.9
        H7GWFDSX7_n02_E459_POOLRET74
        20.0%
        42%
        56.9
        H7GWFDSX7_n02_E460_POOLRET74
        27.0%
        43%
        48.1
        H7GWFDSX7_n02_E461_POOLRET74
        24.8%
        42%
        46.4
        H7GWFDSX7_n02_E462_POOLRET74
        23.3%
        41%
        57.0
        H7GWFDSX7_n02_E463_POOLRET74
        32.6%
        43%
        53.7
        H7GWFDSX7_n02_E464_POOLRET74
        21.9%
        40%
        58.4
        H7GWFDSX7_n02_E466_POOLRET74
        35.1%
        44%
        49.1
        H7GWFDSX7_n02_E472_POOLRET74
        28.6%
        42%
        75.6
        H7GWFDSX7_n02_E477_POOLRET74
        33.2%
        44%
        63.0
        H7GWFDSX7_n02_E478_POOLRET74
        21.4%
        41%
        58.3
        H7GWFDSX7_n02_E479_POOLRET74
        16.3%
        40%
        35.1
        H7GWFDSX7_n02_E480_POOLRET74
        24.2%
        43%
        47.5
        H7GWFDSX7_n02_E484_POOLRET74
        20.4%
        43%
        44.4
        H7GWFDSX7_n02_E487_POOLRET74
        25.5%
        45%
        31.1
        H7GWFDSX7_n02_E494_POOLRET74
        34.4%
        44%
        58.7
        H7GWFDSX7_n02_E498_POOLRET74
        23.4%
        41%
        67.5
        H7GWFDSX7_n02_ECA2936_POOLRET74
        23.7%
        41%
        72.3
        H7GWFDSX7_n02_ECA2939_POOLRET74
        3.9%
        47%
        0.1
        H7GWFDSX7_n02_ECA2945_POOLRET74
        22.2%
        40%
        64.5
        H7GWFDSX7_n02_ECA2949_POOLRET74
        19.2%
        40%
        60.7
        H7GWFDSX7_n02_ECA2956_POOLRET74
        22.5%
        40%
        68.7
        H7GWFDSX7_n02_ECA2963_POOLRET74
        23.3%
        40%
        70.2
        H7GWFDSX7_n02_ECA2964_POOLRET74
        18.8%
        42%
        52.7
        H7GWFDSX7_n02_ECA2967_POOLRET74
        20.1%
        40%
        53.6
        H7GWFDSX7_n02_ECA2968_POOLRET74
        18.5%
        39%
        51.9
        H7GWFDSX7_n02_ECA2970_POOLRET74
        22.4%
        41%
        73.5
        H7GWFDSX7_n02_ECA2974_POOLRET74
        21.1%
        40%
        55.5
        H7GWFDSX7_n02_ECA2975_POOLRET74
        25.0%
        42%
        83.1
        H7GWFDSX7_n02_ECA2983_POOLRET74
        24.2%
        41%
        74.0
        H7GWFDSX7_n02_ECA3006_POOLRET74
        24.9%
        40%
        74.6
        H7GWFDSX7_n02_ECA3037_POOLRET74
        20.8%
        41%
        59.7
        H7GWFDSX7_n02_ECA3045_POOLRET74
        17.3%
        42%
        45.8
        H7GWFDSX7_n02_ECA3049_POOLRET74
        23.4%
        41%
        71.0
        H7GWFDSX7_n02_ECA3053_POOLRET74
        21.6%
        41%
        54.5
        H7GWFDSX7_n02_ECA3073_POOLRET74
        18.3%
        41%
        51.5
        H7GWFDSX7_n02_ECA3077_POOLRET74
        21.1%
        42%
        63.5
        H7GWFDSX7_n02_ECA3085_POOLRET74
        25.8%
        42%
        79.7
        H7GWFDSX7_n02_ECA3087_POOLRET74
        19.3%
        43%
        66.6
        H7GWFDSX7_n02_ECA3088_POOLRET74
        23.1%
        40%
        71.9
        H7GWFDSX7_n02_ECA3108_POOLRET74
        27.0%
        40%
        86.6
        H7GWFDSX7_n02_ECA3110_POOLRET74
        19.0%
        41%
        57.2
        H7GWFDSX7_n02_ECA3111_POOLRET74
        17.2%
        43%
        46.1
        H7GWFDSX7_n02_ECA3112_POOLRET74
        26.7%
        40%
        83.7
        H7GWFDSX7_n02_ECA3113_POOLRET74
        23.9%
        42%
        75.2
        H7GWFDSX7_n02_ECA3114_POOLRET74
        23.5%
        41%
        75.4
        H7GWFDSX7_n02_ECA3115_POOLRET74
        25.6%
        40%
        79.7
        H7GWFDSX7_n02_ECA3116_POOLRET74
        25.1%
        40%
        88.2
        H7GWFDSX7_n02_ECA3117_POOLRET74
        23.3%
        40%
        68.9
        H7GWFDSX7_n02_ECA3118_POOLRET74
        19.1%
        41%
        51.9
        H7GWFDSX7_n02_ECA3119_POOLRET74
        24.9%
        40%
        80.4
        H7GWFDSX7_n02_EG8072_BaerAncestor_POOLRET74_a
        26.0%
        41%
        86.4
        H7GWFDSX7_n02_EG8072_BaerAncestor_POOLRET74_b
        19.1%
        41%
        52.3
        H7GWFDSX7_n02_EG8072_BaerAncestor_POOLRET74_c
        21.0%
        40%
        60.3
        H7GWFDSX7_n02_G.3_POOLRET74
        22.1%
        39%
        63.8
        H7GWFDSX7_n02_G200_POOLRET74
        24.8%
        42%
        51.7
        H7GWFDSX7_n02_G205_POOLRET74
        35.7%
        45%
        51.2
        H7GWFDSX7_n02_G207_POOLRET74
        29.6%
        44%
        54.9
        H7GWFDSX7_n02_G208_POOLRET74
        23.2%
        43%
        49.6
        H7GWFDSX7_n02_G220_POOLRET74
        23.3%
        41%
        64.4
        H7GWFDSX7_n02_G225_POOLRET74
        30.1%
        43%
        62.7
        H7GWFDSX7_n02_G228_POOLRET74
        35.7%
        45%
        57.8
        H7GWFDSX7_n02_G236_POOLRET74
        20.2%
        44%
        50.4
        H7GWFDSX7_n02_G238_POOLRET74
        21.9%
        42%
        60.7
        H7GWFDSX7_n02_G240_POOLRET74
        20.2%
        42%
        39.0
        H7GWFDSX7_n02_G243_POOLRET74
        26.3%
        41%
        83.4
        H7GWFDSX7_n02_G246_POOLRET74
        22.9%
        40%
        66.8
        H7GWFDSX7_n02_G250_POOLRET74
        23.2%
        43%
        59.1
        H7GWFDSX7_n02_G251_POOLRET74
        33.7%
        45%
        47.3
        H7GWFDSX7_n02_G256_POOLRET74
        28.9%
        42%
        61.4
        H7GWFDSX7_n02_G260_POOLRET74
        28.7%
        43%
        61.0
        H7GWFDSX7_n02_G263_POOLRET74
        30.6%
        43%
        54.7
        H7GWFDSX7_n02_G267_POOLRET74
        36.2%
        43%
        89.3
        H7GWFDSX7_n02_G272_POOLRET74
        42.4%
        52%
        66.2
        H7GWFDSX7_n02_G275_POOLRET74
        17.6%
        39%
        46.4
        H7GWFDSX7_n02_G276_POOLRET74
        24.9%
        42%
        65.0
        H7GWFDSX7_n02_G278_POOLRET74
        44.2%
        46%
        52.2
        H7GWFDSX7_n02_G288_POOLRET74
        32.6%
        43%
        65.2
        H7GWFDSX7_n02_G291_POOLRET74
        29.1%
        43%
        48.5
        H7GWFDSX7_n02_G292_POOLRET74
        30.2%
        43%
        62.1
        H7GWFDSX7_n02_G293_POOLRET74
        22.0%
        42%
        47.1
        H7GWFDSX7_n02_G294_POOLRET74
        29.7%
        44%
        34.5
        H7GWFDSX7_n02_J.5_POOLRET74
        26.6%
        41%
        75.1
        H7GWFDSX7_n02_J301_POOLRET74
        49.6%
        47%
        45.1
        H7GWFDSX7_n02_J302_POOLRET74
        64.3%
        50%
        61.9
        H7GWFDSX7_n02_J312_POOLRET74
        25.8%
        39%
        83.1
        H7GWFDSX7_n02_J314_POOLRET74
        21.1%
        40%
        56.0
        H7GWFDSX7_n02_J317_POOLRET74
        39.7%
        46%
        53.1
        H7GWFDSX7_n02_J318_POOLRET74
        38.0%
        45%
        56.4
        H7GWFDSX7_n02_J322_POOLRET74
        19.0%
        43%
        27.8
        H7GWFDSX7_n02_J325_POOLRET74
        40.4%
        45%
        62.0
        H7GWFDSX7_n02_J330_POOLRET74
        29.4%
        43%
        67.4
        H7GWFDSX7_n02_J332_POOLRET74
        36.6%
        45%
        52.2
        H7GWFDSX7_n02_J336_POOLRET74
        38.7%
        44%
        68.1
        H7GWFDSX7_n02_J337_POOLRET74
        20.0%
        39%
        48.5
        H7GWFDSX7_n02_J339_POOLRET74
        22.6%
        42%
        48.2
        H7GWFDSX7_n02_J347_POOLRET74
        34.5%
        45%
        58.4
        H7GWFDSX7_n02_J353_POOLRET74
        35.4%
        45%
        52.8
        H7GWFDSX7_n02_J356_POOLRET74
        44.8%
        48%
        39.5
        H7GWFDSX7_n02_J357_POOLRET74
        52.2%
        48%
        63.7
        H7GWFDSX7_n02_J361_POOLRET74
        48.0%
        46%
        60.4
        H7GWFDSX7_n02_J366_POOLRET74
        18.9%
        39%
        47.0
        H7GWFDSX7_n02_J368_POOLRET74
        41.9%
        46%
        54.0
        H7GWFDSX7_n02_J372_POOLRET74
        26.4%
        42%
        71.8
        H7GWFDSX7_n02_J373_POOLRET74
        22.7%
        40%
        59.6
        H7GWFDSX7_n02_J376_POOLRET74
        32.2%
        43%
        60.1
        H7GWFDSX7_n02_J382_POOLRET74
        53.6%
        49%
        74.2
        H7GWFDSX7_n02_J390_POOLRET74
        16.4%
        44%
        10.0
        H7GWFDSX7_n02_J391_POOLRET74
        49.9%
        47%
        63.1
        H7GWFDSX7_n02_J393_POOLRET74
        29.7%
        43%
        61.9
        H7GWFDSX7_n02_J394_POOLRET74
        40.4%
        47%
        41.8
        H7GWFDSX7_n02_J396_POOLRET74
        39.9%
        45%
        78.2
        H7GWFDSX7_n02_J397_POOLRET74
        46.2%
        47%
        60.1
        H7GWFDSX7_n02_JU360_BaerAncestor_POOLRET74_a
        25.2%
        39%
        67.6
        H7GWFDSX7_n02_JU360_BaerAncestor_POOLRET74_b
        26.8%
        42%
        83.5
        H7GWFDSX7_n02_JU360_BaerAncestor_POOLRET74_c
        22.0%
        41%
        57.4
        H7GWFDSX7_n02_M.4_POOLRET74
        17.9%
        39%
        41.9
        H7GWFDSX7_n02_M.5_POOLRET74
        23.6%
        40%
        77.0
        H7GWFDSX7_n02_M110_POOLRET74
        27.6%
        41%
        75.8
        H7GWFDSX7_n02_M114_POOLRET74
        25.4%
        41%
        80.5
        H7GWFDSX7_n02_M119_POOLRET74
        48.2%
        48%
        60.7
        H7GWFDSX7_n02_M126_POOLRET74
        22.1%
        42%
        54.8
        H7GWFDSX7_n02_M128_POOLRET74
        23.4%
        41%
        55.7
        H7GWFDSX7_n02_M132_POOLRET74
        15.6%
        40%
        37.4
        H7GWFDSX7_n02_M135_POOLRET74
        14.7%
        40%
        30.3
        H7GWFDSX7_n02_M137_POOLRET74
        24.1%
        45%
        65.0
        H7GWFDSX7_n02_M142_POOLRET74
        29.7%
        43%
        53.2
        H7GWFDSX7_n02_M149_POOLRET74
        32.1%
        43%
        59.0
        H7GWFDSX7_n02_M151_POOLRET74
        24.7%
        41%
        71.6
        H7GWFDSX7_n02_M169_POOLRET74
        48.6%
        46%
        70.8
        H7GWFDSX7_n02_M170_POOLRET74
        19.2%
        40%
        46.0
        H7GWFDSX7_n02_M172_POOLRET74
        32.7%
        45%
        42.0
        H7GWFDSX7_n02_M173_POOLRET74
        25.6%
        42%
        62.0
        H7GWFDSX7_n02_M175_POOLRET74
        27.7%
        42%
        57.6
        H7GWFDSX7_n02_M186_POOLRET74
        30.2%
        43%
        51.7
        H7GWFDSX7_n02_M192_POOLRET74
        22.0%
        40%
        60.4
        H7GWFDSX7_n02_MY16_BaerAncestor_POOLRET74_a
        25.7%
        41%
        78.2
        H7GWFDSX7_n02_MY16_BaerAncestor_POOLRET74_b
        28.3%
        39%
        89.2
        H7GWFDSX7_n02_MY16_BaerAncestor_POOLRET74_c
        26.3%
        40%
        77.6
        H7GWFDSX7_n02_undetermined
        47.2%
        42%
        981.6

        Demultiplexing Report


        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 193/193 rows and 2/2 columns.
        LibraryTotal Read CountPortion (%)
        undetermined_library
        981565659
        7.7
        G272_POOLRET74
        66177533
        0.5
        J373_POOLRET74
        59581623
        0.5
        J332_POOLRET74
        52185951
        0.4
        J390_POOLRET74
        10032331
        0.1
        E453_POOLRET74
        52498280
        0.4
        E472_POOLRET74
        75564652
        0.6
        G.3_POOLRET74
        63815472
        0.5
        E413_POOLRET74
        45259622
        0.4
        C083_POOLRET74
        50853720
        0.4
        G263_POOLRET74
        54727493
        0.4
        J391_POOLRET74
        63138478
        0.5
        G293_POOLRET74
        47109846
        0.4
        G228_POOLRET74
        57831428
        0.5
        M169_POOLRET74
        70817446
        0.6
        J397_POOLRET74
        60104553
        0.5
        J302_POOLRET74
        61897106
        0.5
        E461_POOLRET74
        46412509
        0.4
        J312_POOLRET74
        83108050
        0.7
        E464_POOLRET74
        58397658
        0.5
        J325_POOLRET74
        62036492
        0.5
        C029_POOLRET74
        60390017
        0.5
        C.4_POOLRET74
        66364215
        0.5
        M128_POOLRET74
        55687396
        0.4
        C075_POOLRET74
        55583143
        0.4
        G278_POOLRET74
        52198677
        0.4
        E478_POOLRET74
        58349069
        0.5
        E462_POOLRET74
        56999973
        0.4
        C058_POOLRET74
        49233723
        0.4
        J393_POOLRET74
        61947575
        0.5
        E.4_POOLRET74
        71392994
        0.6
        G225_POOLRET74
        62710758
        0.5
        E460_POOLRET74
        48126772
        0.4
        J366_POOLRET74
        46999192
        0.4
        G200_POOLRET74
        51718774
        0.4
        C040_POOLRET74
        48397764
        0.4
        C096_POOLRET74
        56262045
        0.4
        C065_POOLRET74
        41214983
        0.3
        C021_POOLRET74
        52877060
        0.4
        M172_POOLRET74
        41996199
        0.3
        M132_POOLRET74
        37442476
        0.3
        E428_POOLRET74
        78054307
        0.6
        M175_POOLRET74
        57639644
        0.5
        J376_POOLRET74
        60138191
        0.5
        M135_POOLRET74
        30328479
        0.2
        J322_POOLRET74
        27762063
        0.2
        C092_POOLRET74
        50062728
        0.4
        C087_POOLRET74
        37148509
        0.3
        G240_POOLRET74
        38996561
        0.3
        E454_POOLRET74
        46855647
        0.4
        J301_POOLRET74
        45149798
        0.4
        M149_POOLRET74
        58963864
        0.5
        M170_POOLRET74
        45985641
        0.4
        C077_POOLRET74
        47046924
        0.4
        M142_POOLRET74
        53202949
        0.4
        G294_POOLRET74
        34519699
        0.3
        E480_POOLRET74
        47496281
        0.4
        J368_POOLRET74
        53961406
        0.4
        C043_POOLRET74
        63026666
        0.5
        M192_POOLRET74
        60411711
        0.5
        E463_POOLRET74
        53678544
        0.4
        M173_POOLRET74
        61999266
        0.5
        C061_POOLRET74
        61307170
        0.5
        C033_POOLRET74
        45617925
        0.4
        E427_POOLRET74
        51171621
        0.4
        C056_POOLRET74
        68090776
        0.5
        J.5_POOLRET74
        75059304
        0.6
        G246_POOLRET74
        66822484
        0.5
        C028_POOLRET74
        51747278
        0.4
        G207_POOLRET74
        54931183
        0.4
        E449_POOLRET74
        44874992
        0.4
        C049_POOLRET74
        48062317
        0.4
        E484_POOLRET74
        44428020
        0.3
        J361_POOLRET74
        60398738
        0.5
        C063_POOLRET74
        64636023
        0.5
        E479_POOLRET74
        35118243
        0.3
        C069_POOLRET74
        46884735
        0.4
        J396_POOLRET74
        78182160
        0.6
        C088_POOLRET74
        56727617
        0.4
        J339_POOLRET74
        48176420
        0.4
        J317_POOLRET74
        53139134
        0.4
        E450_POOLRET74
        48523977
        0.4
        G288_POOLRET74
        65171783
        0.5
        G205_POOLRET74
        51156982
        0.4
        G291_POOLRET74
        48480028
        0.4
        M126_POOLRET74
        54795930
        0.4
        C000_POOLRET74
        60603233
        0.5
        E459_POOLRET74
        56920674
        0.4
        E.1_POOLRET74
        50900743
        0.4
        E494_POOLRET74
        58681587
        0.5
        J336_POOLRET74
        68142244
        0.5
        J318_POOLRET74
        56381806
        0.4
        J337_POOLRET74
        48507868
        0.4
        G251_POOLRET74
        47338483
        0.4
        C034_POOLRET74
        66476132
        0.5
        M137_POOLRET74
        65047163
        0.5
        E443_POOLRET74
        65010068
        0.5
        C097_POOLRET74
        81756963
        0.6
        E458_POOLRET74
        46891669
        0.4
        E405_POOLRET74
        69871416
        0.5
        E452_POOLRET74
        48846577
        0.4
        J356_POOLRET74
        39500462
        0.3
        M.5_POOLRET74
        77041790
        0.6
        G220_POOLRET74
        64391802
        0.5
        G236_POOLRET74
        50363632
        0.4
        C072_POOLRET74
        68188354
        0.5
        G208_POOLRET74
        49627389
        0.4
        G275_POOLRET74
        46410151
        0.4
        J394_POOLRET74
        41793004
        0.3
        E487_POOLRET74
        31058801
        0.2
        G243_POOLRET74
        83433317
        0.7
        J353_POOLRET74
        52750325
        0.4
        J347_POOLRET74
        58365298
        0.5
        G256_POOLRET74
        61386149
        0.5
        E466_POOLRET74
        49078262
        0.4
        G260_POOLRET74
        60960726
        0.5
        M.4_POOLRET74
        41888708
        0.3
        M119_POOLRET74
        60669251
        0.5
        J372_POOLRET74
        71768490
        0.6
        C055_POOLRET74
        54007788
        0.4
        E446_POOLRET74
        62476789
        0.5
        G276_POOLRET74
        64955263
        0.5
        G250_POOLRET74
        59062701
        0.5
        E409_POOLRET74
        441639916
        3.5
        E412_POOLRET74
        38380744
        0.3
        C024_POOLRET74
        55028215
        0.4
        M114_POOLRET74
        80536003
        0.6
        J314_POOLRET74
        56035464
        0.4
        C027_POOLRET74
        57654100
        0.5
        C085_POOLRET74
        68695964
        0.5
        M151_POOLRET74
        71598362
        0.6
        M186_POOLRET74
        51716331
        0.4
        G292_POOLRET74
        62086762
        0.5
        G238_POOLRET74
        60724864
        0.5
        C047_POOLRET74
        66301922
        0.5
        J357_POOLRET74
        63723996
        0.5
        E423_POOLRET74
        73761109
        0.6
        G267_POOLRET74
        89260265
        0.7
        C014_POOLRET74
        70032057
        0.5
        J330_POOLRET74
        67398934
        0.5
        E455_POOLRET74
        58620436
        0.5
        J382_POOLRET74
        74199870
        0.6
        M110_POOLRET74
        75753009
        0.6
        E477_POOLRET74
        63045971
        0.5
        E498_POOLRET74
        67481651
        0.5
        C066_POOLRET74
        62334297
        0.5
        C013_POOLRET74
        77710058
        0.6
        ECA3053_POOLRET74
        54527549
        0.4
        ECA2963_POOLRET74
        70157353
        0.6
        CB4856_BaerAncestor_POOLRET74_a
        56837187
        0.4
        ECA3085_POOLRET74
        79740656
        0.6
        JU360_BaerAncestor_POOLRET74_a
        67594098
        0.5
        ECA2983_POOLRET74
        74003378
        0.6
        ECA3115_POOLRET74
        79741771
        0.6
        ECA3108_POOLRET74
        86605930
        0.7
        ECA3037_POOLRET74
        59728491
        0.5
        ECA3073_POOLRET74
        51520176
        0.4
        ECA3087_POOLRET74
        66642278
        0.5
        EG8072_BaerAncestor_POOLRET74_a
        86370730
        0.7
        ECA3119_POOLRET74
        80382049
        0.6
        ECA3077_POOLRET74
        63482732
        0.5
        ECA3049_POOLRET74
        71049279
        0.6
        ECA2956_POOLRET74
        68717251
        0.5
        ECA2968_POOLRET74
        51898593
        0.4
        ECA3110_POOLRET74
        57202765
        0.4
        ECA2970_POOLRET74
        73470730
        0.6
        CB4856_BaerAncestor_POOLRET74_b
        84095692
        0.7
        ECA3088_POOLRET74
        71909100
        0.6
        ECA3111_POOLRET74
        46139766
        0.4
        ECA3113_POOLRET74
        75241446
        0.6
        ECA2936_POOLRET74
        72306842
        0.6
        ECA2967_POOLRET74
        53648343
        0.4
        ECA3045_POOLRET74
        45785166
        0.4
        ECA2939_POOLRET74
        90594
        0.0
        ECA3114_POOLRET74
        75387358
        0.6
        CB4856_BaerAncestor_POOLRET74_c
        73290462
        0.6
        ECA3118_POOLRET74
        51930668
        0.4
        ECA2964_POOLRET74
        52748075
        0.4
        ECA2945_POOLRET74
        64493765
        0.5
        MY16_BaerAncestor_POOLRET74_a
        78220952
        0.6
        EG8072_BaerAncestor_POOLRET74_b
        52319665
        0.4
        ECA3117_POOLRET74
        68907669
        0.5
        ECA3112_POOLRET74
        83686321
        0.7
        ECA3116_POOLRET74
        88194239
        0.7
        MY16_BaerAncestor_POOLRET74_b
        89204197
        0.7
        ECA2949_POOLRET74
        60694050
        0.5
        ECA2974_POOLRET74
        55492064
        0.4
        JU360_BaerAncestor_POOLRET74_b
        83509992
        0.7
        EG8072_BaerAncestor_POOLRET74_c
        60334773
        0.5
        ECA3006_POOLRET74
        74594962
        0.6
        ECA2975_POOLRET74
        83075632
        0.7
        MY16_BaerAncestor_POOLRET74_c
        77576779
        0.6
        JU360_BaerAncestor_POOLRET74_c
        57413924
        0.5

        Barcodes of Undetermined Reads


        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here. If your libraries are dual indexed, the two indicies are concatenated.

        Showing 20/20 rows and 2/2 columns.
        Barcode Sequence(s)CountFrequency (%)
        GGGGGGGGAGATCTCG
        93420961.0
        9.5
        GGTGGGAGGTGGTGTT
        20734272.0
        2.1
        AACCCGAGAACCGAAG
        13448142.0
        1.4
        CAGGTAAGCTTTCGAG
        4459008.0
        0.5
        TCTCGGAGAGGTCACT
        2687128.0
        0.3
        GGGGGGAGGTGGTGTT
        2230005.0
        0.2
        CAGGTCAGATTTCGAG
        1690285.0
        0.2
        ACGCGGAGAGTTTAGG
        1563003.0
        0.2
        CCCTCTTCTATGCAAG
        1557019.0
        0.2
        CAGGTAAAATTTCGAG
        1479988.0
        0.1
        TCTGGAACAGTGCTAG
        1349449.0
        0.1
        CTTGACGAGTGTGTGC
        1305753.0
        0.1
        GAAGCCTAATACTGAC
        1260199.0
        0.1
        TAGCTGGCCGCCGGTA
        1252874.0
        0.1
        GGGGTGAGGTGGTGTT
        1117981.0
        0.1
        CAAGTAAGATTTCGAG
        1103852.0
        0.1
        GGCTTACTCGGGAAAG
        1099133.0
        0.1
        TTGCTGGAGGTCCAAT
        1072463.0
        0.1
        GTACCACAGGTTGTGG
        1023881.0
        0.1
        CAGGTAAGGGGGGGGG
        1016914.0
        0.1

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Number of LanesTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        4.0
        15320088576
        12749183295
        7.7
        0.7

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (151bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

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