FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41214524
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19198824.6582656152962No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5255601.2751815355188865No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAG107950.032.9941755
CGGGGTT398950.030.4270631
GGGTTCT201850.027.2200033
CGGGTTT217800.025.5866951
TGTACTC71500.024.0794982
TTGTACT73900.023.3246461
GGGGTTC262450.021.5147272
CCGGGGT350550.020.7310181
CCGGGTT272600.020.5502471
GTACTCT91450.018.8050213
ACGAGAT231300.018.7824487
TCTAGTT104850.017.7772967
GTTCTTT273600.016.870255
GGGTTTT944650.014.5715783
GTAGATA250450.014.49281145
CGTGTAT168650.013.672439145
CTGTGTT177500.013.5630412
TACGAGA290700.013.5052346
CGGCATA302950.012.6571481
GATCCTG110850.012.5901065
Produced by FastQC (version 0.11.5)