FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_8230.TR2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_8230.TR2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25777305
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG303630.11778966032329602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT342750.057.84064145
AGTGTAT477600.047.56726145
TAGTGTT187550.044.849026145
GGGAAAA490300.037.12136145
TCGTGTT204300.035.35111145
CGTGTAT238500.032.896595145
GTAGATA589550.030.232447145
TATAGTT513950.028.344654145
CCGTATA223350.027.011543145
AGATCTA278950.026.956558145
AGTGTAA224700.023.525373145
AACATGC191100.023.2166299
GCTATAA128650.023.165628145
AAGAGTT424100.022.261518145
AAACATG208600.022.137718
CAAACAT217050.021.5430227
GCCAGTA124450.020.5080934
TGGATAT156600.020.1249161
AGCATTA115400.019.9801544
TATCTAG163350.019.6616525
Produced by FastQC (version 0.11.5)