FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_8230.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_8230.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25666380
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG281350.10961810742301796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT340350.058.161953145
AGTGTAT478750.046.785496145
TAGTGTT189850.044.228283145
GGGAAAA479550.038.239925145
TCGTGTT202250.035.5652145
CGTGTAT236050.034.158825145
GTAGATA597850.030.212288145
TATAGTT510400.029.450373145
CCGTATA215450.028.708113145
AGATCTA278600.027.067886145
GCTATAA127450.025.943413145
AGTGTAA219700.023.565153145
AACATGC195700.022.9297189
AAACATG208950.022.3776958
CAAACAT216250.021.7562947
AAGAGTT418200.021.690792145
GCCAGTA120450.021.0068364
AGCATTA113500.020.8239824
TATCTAG164450.019.7494055
AGTAGTC124250.019.3703757
Produced by FastQC (version 0.11.5)