FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_7784.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_7784.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24481343
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG289540.11826965538614445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT302750.055.785004145
AGTGTAT371150.052.14723145
GGGAAAA349450.046.296333145
TAGTGTT164050.040.97657145
TATAGTT327600.038.18364145
CGTGTAT212100.034.223587145
GTAGATA484450.031.374327145
TCGTGTT201650.031.286222145
AAGAGTT281650.031.256548145
AGATCTA217250.028.271948145
CCGTATA150700.028.19788145
TAGTTGT119400.026.7749069
GCTATAA116550.022.896421145
GTACTCT151050.021.7981453
AGTGTAA221300.021.692453145
TGTACTC155700.021.0165522
GAGTGTT224100.020.644812145
TTGTACT161050.020.1546271
GGGAAAT305000.016.952032145
TCGGAAA202950.016.47191145
Produced by FastQC (version 0.11.5)