FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_7784.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_7784.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24294703
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG272960.11235370936619393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT309900.057.089657145
AGTGTAT369900.053.10236145
GGGAAAA345400.045.76393145
TAGTGTT164250.040.657753145
TATAGTT325850.038.451664145
CGTGTAT210150.035.745346145
GTAGATA475350.030.980299145
AAGAGTT272900.030.74107145
TCGTGTT198550.030.274277145
CCGTATA154400.028.270815145
AGATCTA208300.026.942682145
TAGTTGT122300.025.3106299
GCTATAA117600.023.244658145
GTACTCT151650.020.894493
GAGTGTT226100.020.363964145
TGTACTC152800.020.1289982
AGTGTAA227300.020.001257145
TTGTACT159600.019.0601311
TCGGAAA195150.017.314362145
GGGAAAT301000.017.22379145
Produced by FastQC (version 0.11.5)