FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_7431.TR4_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_7431.TR4_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26398928
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG329070.12465278893142934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTT359250.065.05528145
AGATTAT535100.057.986423145
GGGAAAA445000.054.29552145
AGTGTAT348100.054.20287145
TGTTAAA420250.043.603016145
GTAGATA646200.040.981026145
CCGTATA227650.036.47177145
AGATCTA289450.035.248413145
CGTGTAT308200.035.127415145
TGTTAAT490750.033.7633145
AAGAGTT417250.032.86353145
TCGTGTT232700.032.22131145
AGCATTA150200.031.2341374
CGTCGTT112400.023.741083145
TAGTGTT295100.023.51591145
GCATTAG207800.022.9924285
CTGGCTC133800.021.4553879
AGTGTTT284850.021.409119145
AAGATTT382950.020.223122145
CATTAGA226400.019.9485116
Produced by FastQC (version 0.11.5)