FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_7431.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_7431.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26246627
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310160.1181713749351488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTT358550.066.231255145
AGATTAT524600.060.015438145
AGTGTAT348650.057.172413145
GGGAAAA447200.055.16123145
TGTTAAA420350.044.55688145
GTAGATA640200.040.60457145
CCGTATA230100.036.995842145
AGATCTA278850.035.5207145
TGTTAAT485100.034.34948145
CGTGTAT304150.033.853374145
AAGAGTT428650.032.64795145
AGCATTA146100.031.958094
TCGTGTT241200.029.64156145
GCATTAG203450.023.4828365
TAGTGTT284800.022.55772145
CGTCGTT109350.022.346657145
CATTAGA219750.020.61826
CTGGCTC130900.020.6015769
GGTGGTG394650.020.467958145
AGTGTTT276200.019.742203145
Produced by FastQC (version 0.11.5)