FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_6852.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_6852.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25074975
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG303630.12108885452527869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTTA308650.046.655823145
CGTGTAT247250.043.930725145
GGGAAAA319000.035.709057145
TCGTGTT173350.030.576405145
CGTGTAA99700.029.745483145
AAGAGTT233300.024.646278145
TAGTTGT117600.022.8085239
CCGTGTT238450.020.343311145
GTACTCT139500.019.755463
TGTACTC151000.018.5005362
TTGTACT147150.018.160691
TGTTCAA226100.016.676144145
GTAGATA364450.016.33421145
TACTCTA176800.016.281464
TCTAGTT182500.016.2068737
GGGAAAT229050.015.986522145
AGAGCGG148400.015.146792145
TCGGAAA194250.015.080412145
AGAGCCC181100.014.493825145
GTTACGA93600.014.3311484
Produced by FastQC (version 0.11.5)