FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_6852.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_6852.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24929301
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG285040.11433934710002498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT241500.046.328766145
AGTGTTA302000.045.45122145
GGGAAAA320300.034.930992145
TCGTGTT171150.032.283455145
CGTGTAA100100.028.540663145
TAGTTGT121300.025.1606679
AAGAGTT229400.025.097416145
GTACTCT148500.020.8016263
CCGTGTT240300.020.639742145
TGTACTC158650.019.7534712
TTGTACT156600.019.2399941
TCTAGTT187500.017.6688377
TACTCTA180900.017.553914
GGGAAAT226150.016.288048145
TCGGAAA201600.016.14945145
GTAGATA357950.016.043707145
TGTTCAA217850.015.51066145
GTTACGA89150.015.1262034
CTAGTTG224200.014.6149588
CTCTAGT261250.014.3458826
Produced by FastQC (version 0.11.5)