FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_6751.TR4_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_6751.TR4_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23697111
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG408600.1724260818122513No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT345060.145612686711051No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC274610.11588332434278592No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT272620.11504355952926075No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT252600.1065952723097765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT153650.046.391598145
GAGCATT118700.039.6365628
GTACGTT39000.037.744164145
TCGTGTT131350.034.614323145
TGTACTC66700.034.1558572
AGTCTAA113550.032.126363
AGCATTG146200.031.9825049
TTGTACT71500.031.5830631
CCGTATA42150.031.482693145
GCCAGTA106550.031.371324
GCTCAGA126750.031.3455435
AGATCTA66750.030.30898145
TCTAACA125400.029.196925
GAGTCTA126500.028.906892
GTAGCAT108650.028.8241168
TAGCATA111500.028.4123219
GGGAAAA256400.028.309645145
ATAATAC82200.028.2331223
AACGTAT221100.026.689616
TACGACT109300.026.6630427
Produced by FastQC (version 0.11.5)