FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_6751.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_6751.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23588025
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG375610.15923757923777002No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT344670.14612075406906683No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC274360.11631325640870738No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT272420.11549080518610609No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT252380.10699496884542051No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT152000.048.037754145
GAGCATT122600.038.434988
TCGTGTT131100.038.16306145
GTACGTT38550.037.242393145
GCCAGTA101400.033.9613724
TGTACTC71800.033.4387132
CCGTATA42700.033.11338145
TTGTACT73300.032.6795231
GTAGCAT103200.032.383598
TAGCATA105950.031.8853039
ATAATAC77900.031.8326993
AGATCTA64250.030.583902145
AGTCTAA116650.030.519793
AGCATTG156150.029.991369
GCTCAGA126850.029.7755185
GGGAAAA255900.029.638634145
GAGTCTA127250.027.9878622
TCTAACA130550.027.821015
TACGACT106900.026.718797
AACGTAT217650.026.279586
Produced by FastQC (version 0.11.5)