FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_5867.TR1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_5867.TR1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28913034
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334340.11563642888532555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAT260850.050.453476145
AGTGTAT282350.043.96644145
GGGAAAA437050.040.498783145
AGGATAT338550.040.480354145
GGGTGTT266100.032.835793145
GTAGATA561950.031.213573145
CGTGTAT270150.031.13568145
AAGAGTT288800.030.204645145
TCGTGTT238250.028.700146145
AGATCTA228400.027.017101145
CCGTATA202550.026.849382145
GGGAAAT284150.022.277779145
TAGGGTT266100.021.227455145
GAGTGTT307800.021.155145
CGATCTA108250.020.9653174
CTGGCTC213550.019.111999
CTATTAG155050.018.0004088
GATCCTG244100.017.8497895
AGCATTA170250.017.1207964
GATGCCT275600.016.6251015
Produced by FastQC (version 0.11.5)