FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_5867.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_5867.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28793551
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG307700.10686420719695185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAT263600.049.954533145
AGTGTAT276650.044.793587145
AGGATAT338950.042.593185145
GGGAAAA437650.040.244366145
GGGTGTT265950.033.672195145
GTAGATA564000.031.138632145
AAGAGTT287800.030.939413145
CGTGTAT261650.029.98548145
TCGTGTT237000.029.279799145
CCGTATA194450.026.886347145
AGATCTA218150.026.857195145
GGGAAAT279250.021.681923145
GAGTGTT308200.021.151031145
CTGGCTC208650.020.8466859
TAGGGTT263200.020.634806145
CGATCTA108450.020.1892074
GATCCTG242500.018.266355
CTATTAG153250.017.4079538
TAGTTGT127450.016.8366629
AGCATTA178050.016.6948994
Produced by FastQC (version 0.11.5)