FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_4340.TR1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_4340.TR1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26153841
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291990.11164325729440658No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA274530.10496737362592362No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA263950.1009220787111155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT119100.046.210014145
ACGAGCA121650.039.696524
TCACGAG117450.039.5368652
GCTCAGA104900.033.312365
CAATTAG169250.032.124339
TGATCCT172000.032.123054
GGGAAAA220850.029.582405145
CGAGCAA175500.029.206275
GATCCTG203400.026.732785
GCAATTA207150.026.4567098
AGCAATT203450.025.5125757
GGGCACA39350.024.7271791
TCCTGGC228850.023.3461657
CGATCTA30900.022.2923284
GGTTAGA221600.022.0209274
CGGAGTA102050.021.9551184
CAGATTG152400.021.8812338
GTGTGTG116850.021.84339145
GAGCAAT241050.021.8337976
CTGGCTC249500.021.4430859
Produced by FastQC (version 0.11.5)