FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_4340.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_4340.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26053110
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA274220.10525422876577883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG266830.10241771519791687No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA263630.10118945492495907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT122900.048.615505145
TCACGAG120150.038.4611932
ACGAGCA121850.038.3772244
GCTCAGA103100.032.556495
TGATCCT173650.032.0228544
CAATTAG169550.031.7252839
GGGAAAA215150.028.747967145
CGAGCAA174750.028.708085
GATCCTG202950.027.8982665
GGGCACA40200.027.27111
GCAATTA210300.025.9570878
AGCAATT201200.025.4014727
CGATCTA30700.025.2688124
TCCTGGC231150.023.4274187
GGTTAGA217800.023.267984
TTTGATC293500.021.7016872
CAGATTG149600.021.618693
GAGCAAT242100.021.5893256
CTGGCTC251700.021.3419679
CCTGGCT253550.021.071928
Produced by FastQC (version 0.11.5)