FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_4329.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_4329.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28390469
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG485020.17083902347650542No Hit
GCGGTGTCCTACTCTCCCACAACCTATCGGTTGCAGTACCATCGGCGCTG305520.10761357975452958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAT120550.038.795086145
AGGATAT151600.035.153805145
TGTCCTA208100.031.7379765
GATCCTG148950.029.204095
AGATCTA52350.029.08619145
TCGTGTT255150.028.360847145
CTATTAG134300.027.5287088
GGGAAAA296200.027.123074145
CGATCTA83750.025.4529634
CCGTATA52800.025.405165145
GGGTGTT250150.024.405954145
GTGTCCT274950.024.2610684
TCGATCT87450.022.8049033
CGTGTAT175650.022.745089145
GGTGTAT233500.021.892052145
CTGGCTC208900.021.6541739
CCGGGAA680250.021.4429041
CGGTGTC290900.021.34872
AACGTAT755900.021.3095196
TTTGATC259650.021.2077622
Produced by FastQC (version 0.11.5)