FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_4329.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_4329.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28248287
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG444920.1575033558672071No Hit
GCGGTGTCCTACTCTCCCACAACCTATCGGTTGCAGTACCATCGGCGCTG305270.10806672985161897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAT124950.038.99508145
AGGATAT152350.034.45664145
TGTCCTA207300.032.1739655
AGATCTA54150.030.529041145
TCGTGTT251200.028.921768145
GATCCTG155300.028.8958325
CTATTAG131150.028.4114368
CGATCTA79600.027.8700834
CCGTATA53000.026.950518145
GGGAAAA303650.026.839214145
TCGATCT86050.025.9544163
GTGTCCT276350.024.1093734
GGGTGTT250500.023.937265145
CTGGCTC210000.022.093269
CCATTGT464150.021.885504145
TTTGATC265150.021.641332
CGGTGTC286750.021.4025752
TCCTACT323950.021.0352767
CGTGTAT173900.020.930563145
AACGTAT761350.020.8334566
Produced by FastQC (version 0.11.5)