FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_30981.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30981.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24281587
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG313610.12915547900555266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATT235100.079.019424145
TGTGTAT193700.063.45231145
GGGAAAA322400.048.648434145
CTATGTT222100.044.532097145
GTAGATA468900.039.06254145
CCGTATA151450.038.972878145
TGTGCCG275500.038.92727145
TCGTGTT220750.035.541348145
CGTGTAT197700.031.616074145
TGTGCCA305400.028.824183145
ATGTGTT288550.027.265661145
AGATCTA186900.026.18797145
AAGAGTT289150.025.80474145
GTGCCTA288350.025.297953145
TGCCTCA260800.022.326227145
AGAGCGG163200.020.927055145
GCATTAG130600.019.8178425
GTGCCTG214550.017.743452145
CATTAGA138500.017.4819746
CGGTGGG169750.016.958525145
Produced by FastQC (version 0.11.5)