FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_30981.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30981.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24123660
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG295610.12253944882327143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATT226250.077.55985145
TGTGTAT195550.062.741184145
GGGAAAA315350.048.356697145
CTATGTT219800.044.47054145
TGTGCCG272100.040.373306145
GTAGATA460350.038.496677145
CCGTATA153000.036.919533145
TCGTGTT217400.034.38819145
CGTGTAT201000.030.628225145
TGTGCCA305400.028.420734145
AGATCTA191700.027.953262145
ATGTGTT292400.025.443695145
GTGCCTA278950.025.422756145
AAGAGTT296000.024.374828145
TGCCTCA259000.021.61363145
AGAGCGG159550.020.678772145
GCATTAG128350.018.7528725
CGGTGGG162700.018.183722145
CGTCGTT97650.017.153389145
GTGCCTG210200.016.799889145
Produced by FastQC (version 0.11.5)