FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_30979.TR2_batch_8_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30979.TR2_batch_8_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6662188
Sequences flagged as poor quality0
Sequence length151
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG208590.31309533744769735No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT191560.28753316478009927No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC145860.21893708193164169No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC131850.19790795456387603No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG124970.18758101692717166No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT123770.18577980687425813No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC123490.1853595245285783No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG118980.17858997674637822No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT114940.17252590290156927No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA106280.15952717035304317No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG104910.15747078887596688No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG103780.15577464940947328No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT101160.15184200746061205No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA99280.14902011171104748No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA94720.142175513509976No Hit
CGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGC92850.13936862784418574No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91270.13699703460784957No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA87430.1312331624385262No Hit
GGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAG86240.12944696246938692No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC85530.12838124652141308No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG84610.12700031881417936No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA80860.12137153739882453No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC77720.11665837109370075No Hit
GCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCG77330.11607297782650385No Hit
CGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGA77260.11596790724008388No Hit
CCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGC75470.11328110224448784No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG75450.11325108207693929No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA73330.11006894431679203No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC72690.10910829895523814No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA72660.10906326870391528No Hit
GGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCG71220.10690181664041902No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC70690.10610628220038222No Hit
CGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTT68730.10316430578062342No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA68570.10292414444023495No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT68290.10250386209455513No Hit
TGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA67570.10142313606280699No Hit
GCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAG67570.10142313606280699No Hit
GGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACT67560.1014081259790327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAT28850.079.67388145
TGTTAAA34350.079.37133145
ATTAGTT32800.074.721466145
CCGTATA11300.071.2274145
TCGGAAA16500.067.67672145
AGATTAT56700.067.52326145
AAGAGTT31250.058.704723145
CGTCGTT8200.053.056664145
GAAACTA65800.052.3396844
TGTTAAT41800.051.867756145
AGAAACT67100.051.2287563
AAGAAAC68950.050.1972852
AGATCTA25350.048.912594145
GTAGATA60700.048.260876145
TCGTGTT31450.047.956253145
CGTGTAT47950.046.576267145
CGCTCAG17100.046.2161564
GGTCTTA9850.045.641304145
AAGATTT24150.045.638252145
CTAACCA76050.045.37328
Produced by FastQC (version 0.11.5)