FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_30976.TR2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30976.TR2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29629900
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT482430.16281863927991658No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG478620.1615327760134189No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA457450.15438796620980832No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT381910.12889344884727927No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC360490.12166426481358358No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG338470.11423258262768352No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT337100.1137702118468169No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT335850.11334834069639113No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT335180.11312221775976294No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT316650.1068683998258516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAT72050.052.33405145
CGTGTAT76350.048.24693145
AGGATAT69150.048.237038145
TCGTGTT118700.044.350746145
GCTCAGA115650.035.355615
TAGCATA113050.034.0504728
GCCAGTA116150.032.343893
CGCTCAG111650.032.3405044
GTAGCAT119850.031.8763147
CTCGGCT119750.031.4698351
CTTGTTA63050.031.4398331
AGTCTAA177500.030.2353573
GTTACGA70600.029.4749684
TGCCAGT126900.029.4460852
GAGTCTA184300.028.9757332
TCTAACA190500.028.5424425
CAATATA29250.028.013512145
AACGTAT174900.027.5216546
CCATTGT129150.027.062483145
GTCTAAC200050.026.893144