FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_30976.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30976.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29468958
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT481610.16342959937708013No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA456740.1549902103766275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG430640.14613343301788953No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT381280.12938360426588547No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC359890.1221251189132646No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG337900.11466302948343135No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT336780.11428296853930159No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT335380.11380789235913939No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT334690.11357374767034518No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT316150.10728238168448304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAT72550.049.172676145
CGTGTAT76200.047.29307145
AGGATAT68750.046.617146145
TCGTGTT120800.043.03763145
GCTCAGA116900.034.2324035
TAGCATA115100.034.1996658
GCCAGTA115100.033.57813
CTCGGCT119900.032.335221
CGCTCAG109350.032.156024
CCAAATT42650.031.792063145
AGTCTAA181450.031.7697943
GTAGCAT123900.031.4194727
GAGTCTA186650.030.8594672
GTTACGA67400.030.6566264
TGCCAGT126150.030.3628542
TCTAACA197800.030.017225
CTTGTTA61400.029.9161641
GTCTAAC205900.028.2747384
CCTGCCA121350.028.1197761
TACTTCC149900.026.7446145