FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_30893.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30893.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27068461
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG351950.13002216860426605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATT161850.058.199535145
TCGTGTT292600.039.80103145
TGTGCCG376850.034.13565145
AGAGCGG178200.032.187843145
ATGTGTT318600.028.723455145
TGTGTAT250700.026.957813145
GTAGATA387000.026.288742145
CGTGTAT153200.025.46517145
GAGCGTG144500.024.38886145
CGGTGGG160300.023.79443145
GCGTCGG166000.023.021076145
GGGAAAA442150.022.140491145
TGTGCCA200150.020.868439145
AGCATTA98350.019.7582384
CTATGTT232950.019.517668145
TCTCGGG179050.019.399221145
CTGGCTC127400.019.0621789
CAATTAG102650.018.9266159
GGGAAAT218850.018.256947145
GTGCCTA206450.018.159285145
Produced by FastQC (version 0.11.5)