FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_30893.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30893.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26958089
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG325730.12082829758444673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATT156450.060.57408145
TCGTGTT290450.038.120136145
TGTGCCG385800.034.337067145
AGAGCGG173750.030.839437145
ATGTGTT318400.027.850964145
TGTGTAT250100.026.585358145
GTAGATA383400.026.041658145
CGTGTAT149400.025.382706145
GCGTCGG166100.025.144306145
CGGTGGG155250.023.67899145
GAGCGTG143500.023.445145145
AGCATTA99650.022.1178044
GGGAAAA432850.021.60922145
TGTGCCA196350.021.455175145
GCATTAG112250.019.8273535
CAATTAG97450.019.3415159
CTGGCTC128350.018.8082079
CTATGTT232350.018.661448145
TGGATGC171750.018.450113
GGGAAAT211850.018.174099145
Produced by FastQC (version 0.11.5)