FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_30852.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30852.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27017595
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG467490.17303168546275122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAT173300.051.257698145
GCTATAT191150.049.505993145
CCGTATA53650.035.41222145
TAGTGTT181050.033.64359145
GATCCTG135300.030.4357815
GGGAAAA281600.030.102558145
CGTGTAT121300.028.515379145
TCGTGTT264350.027.76021145
CAATTAG124700.024.9395949
TATAGTT214800.024.07004145
GAGTGTT233650.023.71096145
TGTACTC70900.023.3313672
GAGCAAT128350.022.8184266
AGATCTA90950.022.56344145
TCACGAG119350.021.8842492
TTGTACT73350.021.8763331
TGGGATC215200.021.6106262
TTTGATC226900.021.231722
CTGGCTC182550.021.166259
ACGAGCA123900.020.7747214
Produced by FastQC (version 0.11.5)